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Article Open Access

MicroRNAs as outcome predictors in patients with metastatic colorectal cancer treated with bevacizumab in combination with FOLFOX

  • Authors:
    • I. Kiss
    • J. Mlčochová
    • K. Součková
    • P. Fabian
    • A. Poprach
    • J. Halamkova
    • M. Svoboda
    • R. Vyzula
    • O. Slaby
  • View Affiliations / Copyright

    Affiliations: Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, 656 53 Brno, Czech Republic, Department of Molecular Medicine, Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic, Department of Clinical and Experimental Pahology, Masaryk Memorial Cancer Institute, 656 53 Brno, Czech Republic
    Copyright: © Kiss et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 743-750
    |
    Published online on: May 26, 2017
       https://doi.org/10.3892/ol.2017.6255
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Abstract

Bevacizumab is a humanized anti-vascular endothelial growth factor monoclonal antibody, used in combination with a oxaliplatin‑based chemotherapy in the treatment of metastatic colorectal cancer (mCRC). The aim of the present study was to identify microRNA (miRNA)‑based predictive biomarkers of therapy response in order to avoid unnecessary and costly therapy to non‑responding patients. High‑throughput miRNA microarray profiling (Affymetrix miRNA array) was performed on a discovery cohort of patients with mCRC. The discovery cohort was (n=20) divided into either responding (n=10) or non‑responding (n=10) groups of bevacizumab/5‑flourouracil, leucovorin, oxaliplatin (FOLFOX) treatment according to Response Evaluation Criteria in Solid Tumors criteria. Validation of candidate miRNAs was performed on an independent cohort of 41 patients with mCRC using quantitative reverse transcription polymerase chain reaction. Normalized data were subjected to receiver operating characteristic and Kaplan‑Meier analyses. In total, 67 miRNAs were identified to be differentially expressed when miRNA expression was compared between responding and non‑responding patients to bevacizumab/FOLFOX treatment (P<0.05). A total of 7 miRNAs were chosen for independent validation, which confirmed significantly higher expression of miR‑92b‑3p, miR‑3156‑5p, miR‑10a‑5p and miR‑125a‑5p (P<0.005) in tumor tissue of responding patients compared with non‑reponding patients. Using the combination of miRNAs, the present study identified responders to the therapy with sensitivity 82% and specificity 64% (area under the curve = 0.8015). In conclusion, 4 predictive miRNAs associated with progression‑free survival (PFS) were identified in patients with mCRC treated with bevacizumab/FOLFOX. Following further independent validations, detection of these miRNA may enable identification of patients with mCRC who may potentially benefit from the therapy.
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View References

1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Can. 136:E359–E386. 2014. View Article : Google Scholar

2 

van Cutsem E, Nordlinger B and A; ESMO Cervantes; Guidelines Working Group, : Advanced colorectal cancer: ESMO Clinical Practice Guidelines for treatment. Ann Oncol. 21 Suppl 5:v93–v97. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Saltz LB, Clarke S, Díaz-Rubio E, Scheithauer W, Figer A, Wong R, Koski S, Lichinitser M, Yang TS, Rivera F, et al: Bevacizumab in combination with oxaliplatin-based chemotherapy as first-line therapy in metastatic colorectal cancer: A randomized phase III study. J Clin Oncol. 26:2013–2019. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Bennouna J, Sastre J, Arnold D, Österlund P, Greil R, van Cutsem E, von Moos R, Viéitez JM, Bouché O, Borg C, et al: Continuation of bevacizumab after first progression in metastatic colorectal cancer (ML18147): A randomised phase 3 trial. Lancet Oncol. 14:29–37. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Hurwitz HI, Tebbutt NC, Kabbinavar F, Giantonio BJ, Guan ZZ, Mitchell L, Waterkamp D and Tabernero J: Efficacy and safety of bevacizumab in metastatic colorectal cancer: Pooled analysis from seven randomized controlled trials. Oncologist. 18:1004–1012. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Pai SG and Fuloria J: Novel therapeutic agents in the treatment of metastatic colorectal cancer. World J Gastrointest Oncol. 8:99–104. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Spiliopoulou P and Arkenau HT: Rationally designed treatment for metastatic colorectal cancer: Current drug development strategies. World J Gastroenterol. 20:10288–10295. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Bhartiya D and Scaria V: Genomic variations in non-coding RNAs: Structure, function and regulation. Genomics. 107:59–68. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Vychytilova-Faltejskova P, Radova L, Sachlova M, Kosarova Z, Slaba K, Fabian P, Grolich T, Prochazka V, Kala Z, Svoboda M, et al: Serum-based microRNA signatures in early diagnosis and prognosis prediction of colon cancer. Carcinogenesis. 37:941–950. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Mishra PJ: MicroRNAs as promising biomarkers in cancer diagnostics. Biomark Res. 2:192014. View Article : Google Scholar : PubMed/NCBI

11 

Cekaite L, Eide PW, Lind GE, Skotheim RI and Lothe RA: MicroRNAs as growth regulators, their function and biomarker status in colorectal cancer. Oncotarget. 7:6476–6505. 2016.PubMed/NCBI

12 

Boisen MK, Dehlendorff C, Linnemann D, Nielsen BS, Larsen JS, Osterlind K, Nielsen SE, Tarpgaard LS, Qvortrup C, Pfeiffer P, et al: Tissue microRNAs as predictors of outcome in patients with metastatic colorectal cancer treated with first line capecitabine and oxaliplatin with or without bevacizumab. PLoS One. 15:e1094302014. View Article : Google Scholar

13 

Hansen TF, Carlsen AL, Heegaard NH, Sørensen FB and Jakobsen A: Changes in circulating microRNA-126 during treatment with chemotherapy and bevacizumab predicts treatment response in patients with metastatic colorectal cancer. Br J Cancer. 112:624–629. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Eisenhauer EA, Therasse P, Bogaerts J, Schwartz LH, Sargent D, Ford R, Dancey J, Arbuck S, Gwyther S, Mooney M, et al: New response evaluation criteria in solid tumours: Revised RECIST guideline (version 1.1). Eur J Cancer. 45:228–247. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Carvalho BS and Irizarry RA: A framework for oligonucleotide microarray preprocessing. Bioinformatics. 26:2363–2367. 2010. View Article : Google Scholar : PubMed/NCBI

16 

R Development Core Team, . R: a language and environment for statistical computing. R Foundation for Statistical Computing; Vienna, Austria: http://www.r-project.org/March 31–2015

17 

Smyth GK: Limma: linear models for microarray dataBioinformatics and Computational Biology Solutions using R and Bioconductor. Gentleman R, Carey V, Dudoit S, Irizarry R and Huber W: Springer; New York, NY: pp. 397–420. 2005, View Article : Google Scholar

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Vandesompele J, De Preter K, Pattyn F, Poppe B, van Roy N, De Paepe A and Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3:RESEARCH0034. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Schee K, Lorenz S, Worren MM, Günther CC, Holden M, Hovig E, Fodstad O, Meza-Zepeda LA and Flatmark K: Deep sequencing the microRNA transcriptome in colorectal cancer. PLoS One. 8:e661652013. View Article : Google Scholar : PubMed/NCBI

21 

Segersten U, Spector Y, Goren Y, Tabak S and Malmström PU: The role of microRNA profiling in prognosticating progression in Ta and T1 urinary bladder cancer. Urol Oncol. 32:613–618. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Zhi Y, Xie X, Wang R, Wang B, Gu W, Ling Y, Dong W, Zhi F and Liu Y: Serum level of miR-10-5p as a prognostic biomarker for acute myeloid leukemia. Int J Hematol. 102:296–303. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Benson EA, Skaar TC, Liu Y, Nephew KP and Matei D: Carboplatin with decitabine therapy, in recurrent platinum resistant ovarian cancer, alters circulating mirnas concentrations: A pilot study. PLoS One. 10:e01412792015. View Article : Google Scholar : PubMed/NCBI

24 

Gattolliat CH, Uguen A, Pesson M, Trillet K, Simon B, Doucet L, Robaszkiewicz M and Corcos L: MicroRNA and targeted mRNA expression profiling analysis in human colorectal adenomas and adenocarcinomas. Eur J Cancer. 51:409–420. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Ozcan O, Kara M, Yumrutas O, Bozgeyik E, Bozgeyik I and Celik OI: MTUS1 and its targeting miRNAs in colorectal carcinoma: Significant associations. Tumour Biol. 37:6637–6645. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Xu Y, Huang Z and Liu Y: Reduced miR-125a-5p expression is associated with gastric carcinogenesis through the targeting of E2F3. Mol Med Rep. 10:2601–2608. 2014.PubMed/NCBI

27 

Ferracin M, Lupini L, Salamon I, Saccenti E, Zanzi MV, Rocchi A, da Ros L, Zagatti B, Musa G, Bassi C, et al: Absolute quantification of cell-free microRNAs in cancer patients. Oncotarget. 6:14545–14555. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Muz B, de la Puente P, Azab F and Azab AK: The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia (Auckl). 3:83–92. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Agrawal R, Pandey P, Jha P, Dwivedi V, Sarkar C and Kulshreshtha R: Hypoxic signature of microRNAs in glioblastoma: Insights from small RNA deep sequencing. BMC Genomics. 15:6862014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kiss I, Mlčochová J, Součková K, Fabian P, Poprach A, Halamkova J, Svoboda M, Vyzula R and Slaby O: MicroRNAs as outcome predictors in patients with metastatic colorectal cancer treated with bevacizumab in combination with FOLFOX. Oncol Lett 14: 743-750, 2017.
APA
Kiss, I., Mlčochová, J., Součková, K., Fabian, P., Poprach, A., Halamkova, J. ... Slaby, O. (2017). MicroRNAs as outcome predictors in patients with metastatic colorectal cancer treated with bevacizumab in combination with FOLFOX. Oncology Letters, 14, 743-750. https://doi.org/10.3892/ol.2017.6255
MLA
Kiss, I., Mlčochová, J., Součková, K., Fabian, P., Poprach, A., Halamkova, J., Svoboda, M., Vyzula, R., Slaby, O."MicroRNAs as outcome predictors in patients with metastatic colorectal cancer treated with bevacizumab in combination with FOLFOX". Oncology Letters 14.1 (2017): 743-750.
Chicago
Kiss, I., Mlčochová, J., Součková, K., Fabian, P., Poprach, A., Halamkova, J., Svoboda, M., Vyzula, R., Slaby, O."MicroRNAs as outcome predictors in patients with metastatic colorectal cancer treated with bevacizumab in combination with FOLFOX". Oncology Letters 14, no. 1 (2017): 743-750. https://doi.org/10.3892/ol.2017.6255
Copy and paste a formatted citation
x
Spandidos Publications style
Kiss I, Mlčochová J, Součková K, Fabian P, Poprach A, Halamkova J, Svoboda M, Vyzula R and Slaby O: MicroRNAs as outcome predictors in patients with metastatic colorectal cancer treated with bevacizumab in combination with FOLFOX. Oncol Lett 14: 743-750, 2017.
APA
Kiss, I., Mlčochová, J., Součková, K., Fabian, P., Poprach, A., Halamkova, J. ... Slaby, O. (2017). MicroRNAs as outcome predictors in patients with metastatic colorectal cancer treated with bevacizumab in combination with FOLFOX. Oncology Letters, 14, 743-750. https://doi.org/10.3892/ol.2017.6255
MLA
Kiss, I., Mlčochová, J., Součková, K., Fabian, P., Poprach, A., Halamkova, J., Svoboda, M., Vyzula, R., Slaby, O."MicroRNAs as outcome predictors in patients with metastatic colorectal cancer treated with bevacizumab in combination with FOLFOX". Oncology Letters 14.1 (2017): 743-750.
Chicago
Kiss, I., Mlčochová, J., Součková, K., Fabian, P., Poprach, A., Halamkova, J., Svoboda, M., Vyzula, R., Slaby, O."MicroRNAs as outcome predictors in patients with metastatic colorectal cancer treated with bevacizumab in combination with FOLFOX". Oncology Letters 14, no. 1 (2017): 743-750. https://doi.org/10.3892/ol.2017.6255
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