Open Access

Effects of naringin on the expression of miR-19b and cell apoptosis in human hepatocellular carcinoma

  • Authors:
    • Dafei Xie
    • Peiwen Yuan
    • Dong Wang
    • Hua Jin
    • Hui Chen
  • View Affiliations

  • Published online on: May 29, 2017     https://doi.org/10.3892/ol.2017.6278
  • Pages: 1455-1459
  • Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The effects of naringin on the expression of miR-19b and cell apoptosis were investigated in the human hepatocellular carcinoma cell line HepG2. HepG2 cells were treated with varied concentrations of naringin. The effects of naringin on the proliferation of HepG2 cells were observed by an MTT assay, morphological changes of cells were observed by an inverted microscope, cell apoptosis was detected by DAPI staining, miR-19b mRNA levels were determined with RT-PCR, and the expression of Bax and Bcl-2 proteins was examined by western blot assay. MTT results showed that naringin significantly inhibited the proliferation of HepG2 cells. Apoptotic HepG2 cells showed obvious changes in morphology under inverted microscope. DAPI staining suggested that naringin could induce cell shrinkage and nuclear chromatin condensation. RT-PCR results showed that naringin could upregulate the expression of miR-19b mRNA. Finally, western blot suggested that naringin upregulated the expression of Bax protein, but downregulated the expression of Bcl-2 protein. In conclusion, naringin can upregulate the expression of miR-19b mRNA and induce HepG2 cell apoptosis. In addition, it can also upregulate the expression of Bax protein and downregulate the expression of Bcl-2 protein during the process of apoptosis.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Xie D, Yuan P, Wang D, Jin H and Chen H: Effects of naringin on the expression of miR-19b and cell apoptosis in human hepatocellular carcinoma. Oncol Lett 14: 1455-1459, 2017
APA
Xie, D., Yuan, P., Wang, D., Jin, H., & Chen, H. (2017). Effects of naringin on the expression of miR-19b and cell apoptosis in human hepatocellular carcinoma. Oncology Letters, 14, 1455-1459. https://doi.org/10.3892/ol.2017.6278
MLA
Xie, D., Yuan, P., Wang, D., Jin, H., Chen, H."Effects of naringin on the expression of miR-19b and cell apoptosis in human hepatocellular carcinoma". Oncology Letters 14.2 (2017): 1455-1459.
Chicago
Xie, D., Yuan, P., Wang, D., Jin, H., Chen, H."Effects of naringin on the expression of miR-19b and cell apoptosis in human hepatocellular carcinoma". Oncology Letters 14, no. 2 (2017): 1455-1459. https://doi.org/10.3892/ol.2017.6278