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Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer

  • Authors:
    • Shan Shen
    • Jingxia Tang
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1460-1463
    |
    Published online on: May 29, 2017
       https://doi.org/10.3892/ol.2017.6279
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Abstract

The present study describes the effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer. Oral cancer is twice as common in men than women. More than 90% of oral cancers in men and 85% in women are linked to lifestyle and environmental factors. PPP2R2B methylation may be associated with survival and prognosis in patients with gliomas. In tumor cell proliferation and apoptosis, the mechanism of PPP2R2B remains unclear. In the present study, we found that PPP2R2B expression of H1299 cells is significantly decreased after being treated by GA‑13315. KB cells were isolated from patients with oral cancer and treated with GA‑13315 (5 µM). Cells without GA‑13315 treatment served as the control group. An MTT experiment was performed to detect the post‑treatment cell growth between the groups. A flow cytometry was used to detect cell apoptosis. Western blot analysis and quantitative polymerase chain reaction methods were used for detecting the expression of PPP2R2B. Compared with the control group, the cell proliferation of the treatment group slowed after being treated with GA‑13315. The difference was statistically significant (P<0.05). Western blotting showed that the PPP2R2B expression of cells was reduced after being treated with GA‑13315. Compared with the control group, the difference was statistically significant (P<0.05). According to results from the Transwell migration assay, the invasiveness of the KB cells of oral cancer were weakened after being treated by GA‑13315. GA‑13315 can accelerate the apoptosis of oral cancer cells and presents a dose correlation. The biological effect is exerted through the decrease of PPP2R2B.
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View References

1 

Warnakulasuriya S: Living with oral cancer: Epidemiology with particular reference to prevalence and life-style changes that influence survival. Oral Oncol. 46:407–410. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Haron N, Zain RB, Nabillah WM, Saleh A, Kallarakkal TG, Ramanathan A, Sinon SH Mohd, Razak I Abdul and Cheong SC: Mobile phone imaging in low resource settings for early detection of oral cancer and concordance with clinical oral examination. Telemed J E Health. Aug 19–2016.(Epub ahead of print). PubMed/NCBI

3 

Bajpai M, Arora M and Chandolia B: Bioimpedance for Oral Cancer Detection in Clinical Practice and its Applicability in Developing Nations. J Coll Physicians Surg Pak. 26:7212016.PubMed/NCBI

4 

Ishikawa S, Sugimoto M, Kitabatake K, Sugano A, Nakamura M, Kaneko M, Ota S, Hiwatari K, Enomoto A, Soga T, et al: Identification of salivary metabolomic biomarkers for oral cancer screening. Sci Rep. 6:315202016. View Article : Google Scholar : PubMed/NCBI

5 

Petersen PE: Oral cancer prevention and control - the approach of the World Health Organization. Oral Oncol. 45:454–460. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Bömke C and Tudzynski B: Diversity, regulation, and evolution of the gibberellin biosynthetic pathway in fungi compared to plants and bacteria. Phytochemistry. 70:1876–1893. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Chen J, Sun Z, Zhang Y, Zeng X, Qing C, Liu J, Li L and Zhang H: Synthesis of gibberellin derivatives with anti-tumor bioactivities. Bioorg Med Chem Lett. 19:5496–5499. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Zhang Y, Zhang H, Chen J, Zhao H, Zeng X, Zhang H and Qing C: Antitumor and antiangiogenic effects of GA-13315, a gibberellin derivative. Invest New Drugs. 30:8–16. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Conway DI, Petticrew M, Marlborough H, Berthiller J, Hashibe M and Macpherson LM: Socioeconomic inequalities and oral cancer risk: A systematic review and meta-analysis of case-control studies. Int J Cancer. 122:2811–2819. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Petti S: Lifestyle risk factors for oral cancer. Oral Oncol. 45:340–350. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Mo J, Kang M, Ye JX, Chen JB, Zhang HB and Qing C: Gibberellin derivative GA-13315 sensitizes multidrug-resistant cancer cells by antagonizing ABCB1 while agonizes ABCC1. Cancer Chemother Pharmacol. 78:51–61. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Zhang Y, Zhang H, Chen J, Zhao H, Zeng X, Zhang H and Qing C: Antitumor and antiangiogenic effects of GA-13315, a gibberellin derivative. Invest New Drugs. 30:8–16. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Mayer RE, Hendrix P, Cron P, Matthies R, Stone SR, Goris J, Merlevede W, Hofsteenge J and Hemmings BA: Structure of the 55-kDa regulatory subunit of protein phosphatase 2A: Evidence for a neuronal-specific isoform. Biochemistry. 30:3589–3597. 1991. View Article : Google Scholar : PubMed/NCBI

14 

Holmes SE, O'Hearn EE, McInnis MG, Gorelick-Feldman DA, Kleiderlein JJ, Callahan C, Kwak NG, Ingersoll-Ashworth RG, Sherr M, Sumner AJ, et al: Expansion of a novel CAG trinucleotide repeat in the 5′ region of PPP2R2B is associated with SCA12. Nat Genet. 23:391–392. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Marmorstein LY, McLaughlin PJ, Stanton JB, Yan L, Crabb JW and Marmorstein AD: Bestrophin interacts physically and functionally with protein phosphatase 2A. J Biol Chem. 277:30591–30597. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Majchrzak-Celińska A, Słocińska M, Barciszewska AM, Nowak S and Baer-Dubowska W: Wnt pathway antagonists, SFRP1, SFRP2, SOX17, and PPP2R2B, are methylated in gliomas and SFRP1 methylation predicts shorter survival. J Appl Genet. 57:189–197. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shen S and Tang J: Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer. Oncol Lett 14: 1460-1463, 2017.
APA
Shen, S., & Tang, J. (2017). Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer. Oncology Letters, 14, 1460-1463. https://doi.org/10.3892/ol.2017.6279
MLA
Shen, S., Tang, J."Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer". Oncology Letters 14.2 (2017): 1460-1463.
Chicago
Shen, S., Tang, J."Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer". Oncology Letters 14, no. 2 (2017): 1460-1463. https://doi.org/10.3892/ol.2017.6279
Copy and paste a formatted citation
x
Spandidos Publications style
Shen S and Tang J: Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer. Oncol Lett 14: 1460-1463, 2017.
APA
Shen, S., & Tang, J. (2017). Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer. Oncology Letters, 14, 1460-1463. https://doi.org/10.3892/ol.2017.6279
MLA
Shen, S., Tang, J."Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer". Oncology Letters 14.2 (2017): 1460-1463.
Chicago
Shen, S., Tang, J."Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer". Oncology Letters 14, no. 2 (2017): 1460-1463. https://doi.org/10.3892/ol.2017.6279
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