Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma

Yamamoto,Masaaki; Yamasaki,Makoto; Tsukao,Yukiko; Tanaka,Koji; Miyazaki,Yasuhiro; Makino,Tomoki; Takahashi,Tsuyoshi; Kurokawa,Yukinori; Nakajima,Kiyokazu; Takiguchi,Shuji; Mori,Masaki; Doki,Yuichiro

doi:10.3892/ol.2017.6334

August-2017 Volume 14 Issue 2

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August-2017 Volume 14 Issue 2

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Article Open Access

Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma

Authors:
- Masaaki Yamamoto
- Makoto Yamasaki
- Yukiko Tsukao
- Koji Tanaka
- Yasuhiro Miyazaki
- Tomoki Makino
- Tsuyoshi Takahashi
- Yukinori Kurokawa
- Kiyokazu Nakajima
- Shuji Takiguchi
- Masaki Mori
- Yuichiro Doki
View Affiliations / Copyright

Affiliations: Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan

Copyright: © Yamamoto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1581-1587
|
Published online on: June 7, 2017

https://doi.org/10.3892/ol.2017.6334
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Abstract

Poly (ADP-ribose) polymerase-1 (PARP1) plays a vital role in DNA repair and is expected to be an effective target in various malignancies. The aim of the present study was to investigate the clinical and biological significance of PARP1 expression in esophageal squamous cell carcinoma (ESCC). Immunohistochemical (IHC) staining was used to examine the association between PARP1 expression and the clinicopathological features of 86 patients with ESCC. The antitumor effect of small interfering RNA against PARP1 (siPARP1) was examined in a proliferation assay, and the mechanisms of this effect were investigated using western blot analysis and cell cycle assays. Cox multivariate analysis revealed that high expression of PARP1 in IHC staining was a statistically significant independent prognostic factor of poor overall survival (OS). The adjusted hazard ratio for OS in the group with high expression of PARP1 was 2.39 (95% confidence interval, 1.29‑4.44; P=0.0051). In vitro assays showed that siPARP1 significantly decreased proliferation through G2/M arrest. Furthermore, western blot analysis showed that PARP1 was associated with the ataxia telangiectasia mutated-checkpoint kinase 2‑cell division control 25c pathway. The present study suggests that PARP1 expression has a critical role in ESCC progression, and may be a clinical therapeutic target.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma

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