Expression and prognostic significance of miR-375 and miR-221 in liver cancer

Xie,Dafei; Yuan,Peiwen; Wang,Dong; Jin,Hua; Chen,Hui

doi:10.3892/ol.2017.6423

Expression and prognostic significance of miR-375 and miR-221 in liver cancer

Authors:
- Dafei Xie
- Peiwen Yuan
- Dong Wang
- Hua Jin
- Hui Chen
View Affiliations

Affiliations: Department of General Surgery, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, Intensive Care Unit, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China
Published online on: June 20, 2017 https://doi.org/10.3892/ol.2017.6423
Pages: 2305-2309
Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The purpose of this study was to investigate the expression of miR-375 and miR-221 in liver cancer, and examine the correlations with pathological parameters and prognosis. We collected tumors and tumor-adjacent normal tissue from 70 patients with liver cancer admitted to the Department of General Surgery of Zhejiang Hospital. The expression of miR-375 by RT-qPCR was significantly lower in liver cancer tissues than that in the tumor-adjacent normal tissues, and the low expression was correlated with the lymphatic metastasis and TNM stage. By contrast, the expression of miR-221 was significantly higher in liver cancer than that in the tumor-adjacent tissues, and the high expression was correlated with the lymphatic metastasis and TNM stage. The overall 5-year survival rate of patients was 12.9% (9/70). Single-factor survival analysis revealed that miR-375 and miR-221 were the factors affecting the overall survival rate of liver cancer (P<0.05) and multivariate survival analysis by Cox proportional hazards model showed that miR-375 and miR-221 were the independent factors affecting the survival time of patients with liver cancer. Low expression of miR-375 and high expression of miR-221 are closely correlated with the occurrence and development of liver cancer, especially lymphatic metastasis and TNM stage. Thus, miR-375 and miR-221 can serve as reference biomarkers for guiding the treatment of liver cancer and for estimating prognosis.

View Figures

View References

1	Yang JD and Roberts LR: Epidemiology and management of hepatocellular carcinoma. Infect Dis Clin North Am. 24:899–919. 2010. View Article : Google Scholar : PubMed/NCBI
2	Duan XY, Zhang L, Fan JG and Qiao L: NAFLD leads to liver cancer: Do we have sufficient evidence? Cancer Lett. 345:230–234. 2014. View Article : Google Scholar : PubMed/NCBI
3	Karagozian R, Derdák Z and Baffy G: Obesity-associated mechanisms of hepatocarcinogenesis. Metabolism. 63:607–617. 2014. View Article : Google Scholar : PubMed/NCBI
4	Nakagawa H, Umemura A, Taniguchi K, Font-Burgada J, Dhar D, Ogata H, Zhong Z, Valasek MA, Seki E, Hidalgo J, et al: ER stress cooperates with hypernutrition to trigger TNF-dependent spontaneous HCC development. Cancer Cell. 26:331–343. 2014. View Article : Google Scholar : PubMed/NCBI
5	Zhang DY and Friedman SL: Fibrosis-dependent mechanisms of hepatocarcinogenesis. Hepatology. 56:769–775. 2012. View Article : Google Scholar : PubMed/NCBI
6	He G and Karin M: NF-κB and STAT3 - key players in liver inflammation and cancer. Cell Res. 21:159–168. 2011. View Article : Google Scholar : PubMed/NCBI
7	Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI
8	Siomi H and Siomi MC: On the road to reading the RNA-interference code. Nature. 457:396–404. 2009. View Article : Google Scholar : PubMed/NCBI
9	Panera N, Gnani D, Crudele A, Ceccarelli S, Nobili V and Alisi A: MicroRNAs as controlled systems and controllers in non-alcoholic fatty liver disease. World J Gastroenterol. 20:15079–15086. 2014. View Article : Google Scholar : PubMed/NCBI
10	Griffiths-Jones S, Saini HK, van Dongen S and Enright AJ: miRBase: Tools for microRNA genomics. Nucleic Acids Res. 36(Database): D154–D158. Nov 8–2008. View Article : Google Scholar : PubMed/NCBI
11	Yoo BK, Emdad L, Su ZZ, Villanueva A, Chiang DY, Mukhopadhyay ND, Mills AS, Waxman S, Fisher RA, Llovet JM, et al: Astrocyte elevated gene-1 regulates hepatocellular carcinoma development and progression. J Clin Invest. 119:465–477. 2009. View Article : Google Scholar : PubMed/NCBI
12	Vigouroux C, Auclair M, Dubosclard E, Pouchelet M, Capeau J, Courvalin JC and Buendia B: Nuclear envelope disorganization in fibroblasts from lipodystrophic patients with heterozygous R482Q/W mutations in the lamin A/C gene. J Cell Sci. 114:4459–4468. 2001.PubMed/NCBI
13	Goldman RD, Gruenbaum Y, Moir RD, Shumaker DK and Spann TP: Nuclear lamins: Building blocks of nuclear architecture. Genes Dev. 16:533–547. 2002. View Article : Google Scholar : PubMed/NCBI
14	He XX, Chang Y, Meng FY, Wang MY, Xie QH, Tang F, Li PY, Song YH and Lin JS: MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo. Oncogene. 31:3357–3369. 2012. View Article : Google Scholar : PubMed/NCBI
15	He XX, Guo AY, Xu CR, Chang Y, Xiang GY, Gong J, Dan ZL, Tian DA, Liao JZ and Lin JS: Bioinformatics analysis identifies miR-221 as a core regulator in hepatocellular carcinoma and its silencing suppresses tumor properties. Oncol Rep. 32:1200–1210. 2014.PubMed/NCBI
16	Tang W, Zhu J, Su S, Wu W, Liu Q, Su F and Yu F: MiR-27 as a prognostic marker for breast cancer progression and patient survival. PLoS One. 7:e517022012. View Article : Google Scholar : PubMed/NCBI
17	Zhu Z, Zhang X, Wang G and Zheng H: Role of microRNAs in hepatocellular carcinoma. Hepat Mon. 14:e186722014. View Article : Google Scholar : PubMed/NCBI
18	Huang YS, Dai Y, Yu XF, Bao SY, Yin YB, Tang M and Hu CX: Microarray analysis of microRNA expression in hepatocellular carcinoma and non-tumorous tissues without viral hepatitis. J Gastroenterol Hepatol. 23:87–94. 2008. View Article : Google Scholar : PubMed/NCBI
19	Cohen M, Lee KK, Wilson KL and Gruenbaum Y: Transcriptional repression, apoptosis, human disease and the functional evolution of the nuclear lamina. Trends Biochem Sci. 26:41–47. 2001. View Article : Google Scholar : PubMed/NCBI
20	Yuan Q, Loya K, Rani B, Möbus S, Balakrishnan A, Lamle J, Cathomen T, Vogel A, Manns MP, Ott M, et al: MicroRNA-221 overexpression accelerates hepatocyte proliferation during liver regeneration. Hepatology. 57:299–310. 2013. View Article : Google Scholar : PubMed/NCBI
21	Pineau P, Volinia S, McJunkin K, Marchio A, Battiston C, Terris B, Mazzaferro V, Lowe SW, Croce CM and Dejean A: miR-221 overexpression contributes to liver tumorigenesis. Proc Natl Acad Sci USA. 107:pp. 264–269. 2010; View Article : Google Scholar : PubMed/NCBI
22	Li Y, Jiang Q, Xia N, Yang H and Hu C: Decreased expression of microRNA-375 in nonsmall cell lung cancer and its clinical significance. J Int Med Res. 40:1662–1669. 2012. View Article : Google Scholar : PubMed/NCBI
23	Kong KL, Kwong DL, Chan TH, Law SY, Chen L, Li Y, Qin YR and Guan XY: MicroRNA-375 inhibits tumour growth and metastasis in oesophageal squamous cell carcinoma through repressing insulin-like growth factor 1 receptor. Gut. 61:33–42. 2012. View Article : Google Scholar : PubMed/NCBI