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Article

Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma

  • Authors:
    • Ping Ai
    • Zhiping Li
    • Yong Jiang
    • Changping Song
    • Lin Zhang
    • Huaizhong Hu
    • Tao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Head and Neck Oncology, Cancer Center, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Abdominal Oncology, Cancer Center, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Laboratory of Molecular and Translational Medicine, Department of Obstetrics and Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 2458-2462
    |
    Published online on: June 22, 2017
       https://doi.org/10.3892/ol.2017.6461
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Abstract

Nuclear antigen-1 (NA1) protein of Epstein‑Barr virus (EBV) is expressed in EBV‑infected cells in the microenvironment of cancer. Since immune cells infiltrate abundantly in nasopharyngeal carcinoma (NPC) tumor tissues, we hypothesized that the local tumor microenvironment may perform an important role in the production of antibodies directed at NA1. Furthermore, we hypothesized that anti‑NA1 antibody originating in the local microenvironment could be secreted into the saliva of patients with NPC. In the present study, 20 healthy controls and 39 patients with NPC treated with intensity‑modulated radiation therapy were recruited for the study. Saliva and serum samples were collected from the NPC patients, and nasopharyngeal tissue samples from the patients with NPC. The titers of anti‑NA1 antibody [immunoglobulin A (IgA)] were determined by ELISA. Expression of NA1, human leukocyte antigen‑antigen D related (HLA‑DR), cluster of differentiation (CD)80, CD86, CD3, CD4, CD19 and IgA was detected by immunohistochemical staining on paraffin‑embedded nasopharyngeal tissue sections. Anti‑NA1 antibodies were detected in the serum and saliva samples of the patients with NPC. In infiltrating cells, expression of HLA‑DR, CD80, CD86, CD3, CD4, CD19 and IgA was detected, indicating that dendritic cells, T lymphocytes and B lymphocytes were all present in the local tumor tissues. Furthermore, expression of EBNA1 protein was detected on the membrane of the NPC tumor cells. Therefore, the NPC tumor microenvironment has the potential to initiate a humoral response to EBNA1 by producing IgA antibodies.
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Copy and paste a formatted citation
Spandidos Publications style
Ai P, Li Z, Jiang Y, Song C, Zhang L, Hu H and Wang T: Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma. Oncol Lett 14: 2458-2462, 2017.
APA
Ai, P., Li, Z., Jiang, Y., Song, C., Zhang, L., Hu, H., & Wang, T. (2017). Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma. Oncology Letters, 14, 2458-2462. https://doi.org/10.3892/ol.2017.6461
MLA
Ai, P., Li, Z., Jiang, Y., Song, C., Zhang, L., Hu, H., Wang, T."Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma". Oncology Letters 14.2 (2017): 2458-2462.
Chicago
Ai, P., Li, Z., Jiang, Y., Song, C., Zhang, L., Hu, H., Wang, T."Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma". Oncology Letters 14, no. 2 (2017): 2458-2462. https://doi.org/10.3892/ol.2017.6461
Copy and paste a formatted citation
x
Spandidos Publications style
Ai P, Li Z, Jiang Y, Song C, Zhang L, Hu H and Wang T: Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma. Oncol Lett 14: 2458-2462, 2017.
APA
Ai, P., Li, Z., Jiang, Y., Song, C., Zhang, L., Hu, H., & Wang, T. (2017). Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma. Oncology Letters, 14, 2458-2462. https://doi.org/10.3892/ol.2017.6461
MLA
Ai, P., Li, Z., Jiang, Y., Song, C., Zhang, L., Hu, H., Wang, T."Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma". Oncology Letters 14.2 (2017): 2458-2462.
Chicago
Ai, P., Li, Z., Jiang, Y., Song, C., Zhang, L., Hu, H., Wang, T."Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma". Oncology Letters 14, no. 2 (2017): 2458-2462. https://doi.org/10.3892/ol.2017.6461
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