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Experimental study of a novel tumstatin on C6 brain glioma in vitro

  • Authors:
    • Pengguo Zhang
    • Qingwei Zhou
    • Lin Tian
    • Xiangyu Zhou
    • Yue Zhou
    • Jiajun Chen
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, Jilin University Second Hospital, Changchun, Jilin 130022, P.R. China, Department of Biology and Medical Engineering, School of Regenerative Medicine, Jilin University, Changchun, Jilin 130022, P.R. China, Department of Biology and Medical Engineering, School of Pharmacy, Jilin University, Changchun, Jilin 130022, P.R. China, Department of Neurology, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130022, P.R. China, Department of Biological Preparation, School of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu 210046, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2845-2851
    |
    Published online on: June 30, 2017
       https://doi.org/10.3892/ol.2017.6507
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Abstract

To investigate the effect of a novel tumstatin on C6 brain glioma cells, the MTT method was used to detect C6 glioma cell proliferation activity at different time periods (12, 48 and 72 h). Cell cycle distribution and apoptosis rate were detected by flow cytometry, and the acridine orange/ethidium bromide staining method was used to detect apoptosis and mitochondrial membrane potential by fluorescence microscopy. Novel tumstatin had an evident inhibitory effect on C6 glioma cells, and the most notable impact emerged after 48 h. The following were observed under the fluorescence microscope: Characteristic morphological changes of cell apoptosis were typically observed in the novel tumstatin (2,000 µg/ml) group; mitochondrial membrane potential decreased significantly (P<0.05); the cells in the G0/G1 phase significantly increased (P<0.05); and the number of cells in the S phase was reduced. There was an increase in cell apoptosis rate in the novel tumstatin (2,000 µg/ml) group compared with the novel tumstatin (1,000 µg/ml) group and the Mock group, and the data were statistically significant (P<0.05). Novel tumstatin may reduce the mitochondrial membrane potential, inducing cell apoptosis, and thereby exerting antitumor activity.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang P, Zhou Q, Tian L, Zhou X, Zhou Y and Chen J: Experimental study of a novel tumstatin on C6 brain glioma in vitro. Oncol Lett 14: 2845-2851, 2017.
APA
Zhang, P., Zhou, Q., Tian, L., Zhou, X., Zhou, Y., & Chen, J. (2017). Experimental study of a novel tumstatin on C6 brain glioma in vitro. Oncology Letters, 14, 2845-2851. https://doi.org/10.3892/ol.2017.6507
MLA
Zhang, P., Zhou, Q., Tian, L., Zhou, X., Zhou, Y., Chen, J."Experimental study of a novel tumstatin on C6 brain glioma in vitro". Oncology Letters 14.3 (2017): 2845-2851.
Chicago
Zhang, P., Zhou, Q., Tian, L., Zhou, X., Zhou, Y., Chen, J."Experimental study of a novel tumstatin on C6 brain glioma in vitro". Oncology Letters 14, no. 3 (2017): 2845-2851. https://doi.org/10.3892/ol.2017.6507
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang P, Zhou Q, Tian L, Zhou X, Zhou Y and Chen J: Experimental study of a novel tumstatin on C6 brain glioma in vitro. Oncol Lett 14: 2845-2851, 2017.
APA
Zhang, P., Zhou, Q., Tian, L., Zhou, X., Zhou, Y., & Chen, J. (2017). Experimental study of a novel tumstatin on C6 brain glioma in vitro. Oncology Letters, 14, 2845-2851. https://doi.org/10.3892/ol.2017.6507
MLA
Zhang, P., Zhou, Q., Tian, L., Zhou, X., Zhou, Y., Chen, J."Experimental study of a novel tumstatin on C6 brain glioma in vitro". Oncology Letters 14.3 (2017): 2845-2851.
Chicago
Zhang, P., Zhou, Q., Tian, L., Zhou, X., Zhou, Y., Chen, J."Experimental study of a novel tumstatin on C6 brain glioma in vitro". Oncology Letters 14, no. 3 (2017): 2845-2851. https://doi.org/10.3892/ol.2017.6507
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