Catalpol promotes cellular apoptosis in human HCT116 colorectal cancer cells via microRNA‑200 and the downregulation of PI3K‑Akt signaling pathway

  • Authors:
    • Lan Liu
    • Hongwei Gao
    • Hongbo Wang
    • Yuan Zhang
    • Weihua Xu
    • Sen Lin
    • Hongjuan Wang
    • Qiong Wu
    • Jianqiang Guo
  • View Affiliations

  • Published online on: July 15, 2017     https://doi.org/10.3892/ol.2017.6580
  • Pages: 3741-3747
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Abstract

Catalpol is an effective active ingredient that functions as a diuretic and laxative, and exhibits blood sugar‑lowering, liver protective, anti‑aging and anticancer effects. In traditional Chinese medicine, catalpol is believed to be Yin nourishing. The anticancer effect of catalpol on human HCT116 colorectal cancer cells were investigated and the mechanism of action was evaluated. Cellular viability was detected using an MTT assay. Caspase‑3 and caspase‑9 activity, cellular apoptosis and nucleic morphology were analyzed using caspase‑3 and caspase‑9 activity assay kits, flow cytometric assays and DAPI staining assay, respectively. Western blot analysis was used to measure the protein expressions of phosphatidylinositol 3‑kinase (PI3K), phosphorylated‑protein kinase B (p‑Akt) and Akt. Expression of microRNA‑200 was detected using the reverse transcription‑quantitative polymerase chain reaction. HCT116 cells were incubated with PI3K inhibitors in order to analyze the effect of catalpol on cell proliferation. Catalpol was able to inhibit HCT116 cell proliferation. Furthermore, catalpol induced apoptosis in HCT116 cells, which depended on the increased activities of caspase‑3 and ‑9. In addition, catalpol reduced the expression of PI3K, p‑Akt and Akt in HCT116 cells. However, downregulation of PI3K/Akt decreased the viability of HCT116 cells following treatment with catalpol and enhanced microRNA‑200 expression. Catalpol promoted cellular apoptosis in human HCT116 colorectal cancer cells through upregulation of microRNA‑200 expression, which depended on a downregulation of the phosphatase and tensin homolog/PI3K‑Akt signaling pathway.
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September-2017
Volume 14 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu L, Gao H, Wang H, Zhang Y, Xu W, Lin S, Wang H, Wu Q and Guo J: Catalpol promotes cellular apoptosis in human HCT116 colorectal cancer cells via microRNA‑200 and the downregulation of PI3K‑Akt signaling pathway. Oncol Lett 14: 3741-3747, 2017
APA
Liu, L., Gao, H., Wang, H., Zhang, Y., Xu, W., Lin, S. ... Guo, J. (2017). Catalpol promotes cellular apoptosis in human HCT116 colorectal cancer cells via microRNA‑200 and the downregulation of PI3K‑Akt signaling pathway. Oncology Letters, 14, 3741-3747. https://doi.org/10.3892/ol.2017.6580
MLA
Liu, L., Gao, H., Wang, H., Zhang, Y., Xu, W., Lin, S., Wang, H., Wu, Q., Guo, J."Catalpol promotes cellular apoptosis in human HCT116 colorectal cancer cells via microRNA‑200 and the downregulation of PI3K‑Akt signaling pathway". Oncology Letters 14.3 (2017): 3741-3747.
Chicago
Liu, L., Gao, H., Wang, H., Zhang, Y., Xu, W., Lin, S., Wang, H., Wu, Q., Guo, J."Catalpol promotes cellular apoptosis in human HCT116 colorectal cancer cells via microRNA‑200 and the downregulation of PI3K‑Akt signaling pathway". Oncology Letters 14, no. 3 (2017): 3741-3747. https://doi.org/10.3892/ol.2017.6580