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Identification of target gene of venous thromboembolism in patients with lymphoma via microarray analysis

  • Authors:
    • Pengfei Liu
    • Wenhua Jiang
    • Huilai Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Lymphoma, Sino‑US Center of Lymphoma and Leukemia, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, P.R. China, Department of Radiotherapy, Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3313-3318
    |
    Published online on: July 20, 2017
       https://doi.org/10.3892/ol.2017.6625
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Abstract

Patients with lymphoma are at high risk of developing venous thromboembolism (VTE). The purpose of the present study was to identify the target gene associated with VTE for patients with lymphoma. Microarray data was downloaded from the gene expression omnibus database (GSE17078), which comprised the control group, 27 normal blood outgrowth endothelial cell (BOEC) samples, and the case group, 3 BOEC samples of venous thrombosis with protein C deficiency. Differentially expressed genes (DEGs) were identified by the Limma package of R. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses were performed via the database for annotation, visualization and integrated discovery. Differentially coexpressed pairs were identified by the DCGL package of R. The subsequent protein‑protein interaction (PPI) networks and gene coexpression networks were constructed by the Search Tool for the Retrieval of Interacting Genes/Proteins database, and were visualized by Cytoscape software. A total of 110 DEGs were obtained, including 73 upregulated and 37 downregulated genes. GO and KEGG pathway enrichment analyses identified 132 significant GO terms and 9 significant KEGG pathways. In total, 97 PPI pairs for PPI network and 309 differential coexpression pairs for the gene coexpression network were obtained. Additionally, the connective tissue growth factor (CTGF) gene was closely connected with other genes in the two networks. A total of 2 KEGG pathways were associated with VTE and CTGF may be the target gene of VTE in patients with lymphoma. The present study may identify the molecular mechanism of VTE, but additional clinical study is required to validate the results.
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Copy and paste a formatted citation
Spandidos Publications style
Liu P, Jiang W and Zhang H: Identification of target gene of venous thromboembolism in patients with lymphoma via microarray analysis. Oncol Lett 14: 3313-3318, 2017.
APA
Liu, P., Jiang, W., & Zhang, H. (2017). Identification of target gene of venous thromboembolism in patients with lymphoma via microarray analysis. Oncology Letters, 14, 3313-3318. https://doi.org/10.3892/ol.2017.6625
MLA
Liu, P., Jiang, W., Zhang, H."Identification of target gene of venous thromboembolism in patients with lymphoma via microarray analysis". Oncology Letters 14.3 (2017): 3313-3318.
Chicago
Liu, P., Jiang, W., Zhang, H."Identification of target gene of venous thromboembolism in patients with lymphoma via microarray analysis". Oncology Letters 14, no. 3 (2017): 3313-3318. https://doi.org/10.3892/ol.2017.6625
Copy and paste a formatted citation
x
Spandidos Publications style
Liu P, Jiang W and Zhang H: Identification of target gene of venous thromboembolism in patients with lymphoma via microarray analysis. Oncol Lett 14: 3313-3318, 2017.
APA
Liu, P., Jiang, W., & Zhang, H. (2017). Identification of target gene of venous thromboembolism in patients with lymphoma via microarray analysis. Oncology Letters, 14, 3313-3318. https://doi.org/10.3892/ol.2017.6625
MLA
Liu, P., Jiang, W., Zhang, H."Identification of target gene of venous thromboembolism in patients with lymphoma via microarray analysis". Oncology Letters 14.3 (2017): 3313-3318.
Chicago
Liu, P., Jiang, W., Zhang, H."Identification of target gene of venous thromboembolism in patients with lymphoma via microarray analysis". Oncology Letters 14, no. 3 (2017): 3313-3318. https://doi.org/10.3892/ol.2017.6625
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