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The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells

  • Authors:
    • Ji-Ping Teng
    • Zhi-Ying Yang
    • Yu-Ming Zhu
    • Da Ni
    • Zhi-Jun Zhu
    • Xiao-Qiang Li
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Department of Thoracic and Cardiovascular Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China, Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, P.R. China
    Copyright: © Teng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4613-4618
    |
    Published online on: August 7, 2017
       https://doi.org/10.3892/ol.2017.6729
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Abstract

Lung cancer is a leading cause of cancer-related mortalities worldwide. In the present study, a comparison of To determine the roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells expression levels between normal lung tissues and lung cancer tissues were compared using immunoblotting, and CCK-8 and Transwell assays. Lung cancer tissues had a decreased ARHGAP10 mRNA expression level compared to the adjacent normal tissues. The ectopic expression of ARHGAP10 significantly suppressed the migration, invasion and proliferation of lung cancer cells. Gene set enrichment analysis revealed that metastasis and Wnt signaling pathways were negatively correlated with ARHGAP10 expression. Immunoblotting analysis revealed that ARHGAP10 overexpression inhibited metastasis [matrix metalloproteinase (MMP)-2, MMP-9 and VEGF] and the expression of Wnt pathway-related proteins (β-catenin and c-Myc). Moreover, the stimulation effects of lithium chloride, a GSK3β inhibitor, on the accumulation of β-catenin were notably suppressed by ARHGAP10 overexpression. Collectively, ARHGAP10 acts to suppress tumor within lung cancer by affecting metastasis and Wnt signaling pathways. The results therefore suggest that ARHGAP10 is a potentially attractive target for the treatment of lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Teng J, Yang Z, Zhu Y, Ni D, Zhu Z and Li X: The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells. Oncol Lett 14: 4613-4618, 2017.
APA
Teng, J., Yang, Z., Zhu, Y., Ni, D., Zhu, Z., & Li, X. (2017). The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells. Oncology Letters, 14, 4613-4618. https://doi.org/10.3892/ol.2017.6729
MLA
Teng, J., Yang, Z., Zhu, Y., Ni, D., Zhu, Z., Li, X."The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells". Oncology Letters 14.4 (2017): 4613-4618.
Chicago
Teng, J., Yang, Z., Zhu, Y., Ni, D., Zhu, Z., Li, X."The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells". Oncology Letters 14, no. 4 (2017): 4613-4618. https://doi.org/10.3892/ol.2017.6729
Copy and paste a formatted citation
x
Spandidos Publications style
Teng J, Yang Z, Zhu Y, Ni D, Zhu Z and Li X: The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells. Oncol Lett 14: 4613-4618, 2017.
APA
Teng, J., Yang, Z., Zhu, Y., Ni, D., Zhu, Z., & Li, X. (2017). The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells. Oncology Letters, 14, 4613-4618. https://doi.org/10.3892/ol.2017.6729
MLA
Teng, J., Yang, Z., Zhu, Y., Ni, D., Zhu, Z., Li, X."The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells". Oncology Letters 14.4 (2017): 4613-4618.
Chicago
Teng, J., Yang, Z., Zhu, Y., Ni, D., Zhu, Z., Li, X."The roles of ARHGAP10 in the proliferation, migration and invasion of lung cancer cells". Oncology Letters 14, no. 4 (2017): 4613-4618. https://doi.org/10.3892/ol.2017.6729
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