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Article Open Access

Inhibitory effect of carboplatin in combination with bevacizumab on human retinoblastoma in an in vitro and in vivo model

  • Authors:
    • Qi Zhang
    • Yong Cheng
    • Lvzhen Huang
    • Yujing Bai
    • Jianhong Liang
    • Xiaoxin Li
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The People's Hospital, Peking University, Beijing 100044, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5326-5332
    |
    Published online on: August 25, 2017
       https://doi.org/10.3892/ol.2017.6827
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Abstract

Retinoblastoma is the most common type of malignant intraocular tumor in children, and angiogenesis is required for tumor growth and expansion. The present study investigated whether use of the vascular endothelial growth factor inhibitor antibody bevacizumab can increase the inhibitory effect of carboplatin on human retinoblastoma Y79 cells. This was investigated using in vitro and in vivo models. Cell proliferation was assayed using a Cell Counting Kit‑8 assay, which tested different concentrations of carboplatin in combination with bevacizumab. Cell apoptosis and cell cycle were analyzed using flow cytometry. Protein levels of related signaling pathway molecules were determined by western blotting. The present study used an intravitreal retinoblastoma mouse model for the in vivo study (n=40). Tumors were analyzed histologically. The present study revealed that combining bevacizumab and carboplatin in an in vitro culture of Y79 cells led to a higher inhibition of cellular proliferation than carboplatin alone (P<0.05). The drug combination caused increased apoptosis, and a greater inhibition of the phosphoinositide 3‑kinase (PI3K)/protein kinase B (Akt) and mitogen activated protein kinase (MAPK)/extracellular signal‑regulated kinase (ERK) pathways. This combination also effectively inhibited tumor growth in vivo (P<0.05). These results demonstrate that a combination of carboplatin and bevacizumab results in a greater antitumor effect in advanced human retinoblastoma in vitro and in vivo by inhibiting the PI3K/Akt and MAPK/ERK pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Q, Cheng Y, Huang L, Bai Y, Liang J and Li X: Inhibitory effect of carboplatin in combination with bevacizumab on human retinoblastoma in an in vitro and in vivo model. Oncol Lett 14: 5326-5332, 2017.
APA
Zhang, Q., Cheng, Y., Huang, L., Bai, Y., Liang, J., & Li, X. (2017). Inhibitory effect of carboplatin in combination with bevacizumab on human retinoblastoma in an in vitro and in vivo model. Oncology Letters, 14, 5326-5332. https://doi.org/10.3892/ol.2017.6827
MLA
Zhang, Q., Cheng, Y., Huang, L., Bai, Y., Liang, J., Li, X."Inhibitory effect of carboplatin in combination with bevacizumab on human retinoblastoma in an in vitro and in vivo model". Oncology Letters 14.5 (2017): 5326-5332.
Chicago
Zhang, Q., Cheng, Y., Huang, L., Bai, Y., Liang, J., Li, X."Inhibitory effect of carboplatin in combination with bevacizumab on human retinoblastoma in an in vitro and in vivo model". Oncology Letters 14, no. 5 (2017): 5326-5332. https://doi.org/10.3892/ol.2017.6827
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Q, Cheng Y, Huang L, Bai Y, Liang J and Li X: Inhibitory effect of carboplatin in combination with bevacizumab on human retinoblastoma in an in vitro and in vivo model. Oncol Lett 14: 5326-5332, 2017.
APA
Zhang, Q., Cheng, Y., Huang, L., Bai, Y., Liang, J., & Li, X. (2017). Inhibitory effect of carboplatin in combination with bevacizumab on human retinoblastoma in an in vitro and in vivo model. Oncology Letters, 14, 5326-5332. https://doi.org/10.3892/ol.2017.6827
MLA
Zhang, Q., Cheng, Y., Huang, L., Bai, Y., Liang, J., Li, X."Inhibitory effect of carboplatin in combination with bevacizumab on human retinoblastoma in an in vitro and in vivo model". Oncology Letters 14.5 (2017): 5326-5332.
Chicago
Zhang, Q., Cheng, Y., Huang, L., Bai, Y., Liang, J., Li, X."Inhibitory effect of carboplatin in combination with bevacizumab on human retinoblastoma in an in vitro and in vivo model". Oncology Letters 14, no. 5 (2017): 5326-5332. https://doi.org/10.3892/ol.2017.6827
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