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Article Open Access

Synergistic anti‑proliferative and pro‑apoptotic activities of 5F and cisplatin in human non‑small cell lung cancer NCI‑H23 cells

  • Authors:
    • Yuchan Li
    • Wende Li
    • Wusheng Deng
    • Yuhong Gan
    • Kefeng Wu
    • Jie Sun
  • View Affiliations / Copyright

    Affiliations: Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang, Guangdong 524023, P.R. China, Department of Respiratory Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5347-5353
    |
    Published online on: August 28, 2017
       https://doi.org/10.3892/ol.2017.6848
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Abstract

Two‑drug combination chemotherapy, often inclu­ding cisplatin and one other drug, remains the standard of care for patients with advanced non‑small cell lung cancer (NSCLC). To improve the treatment of late‑stage NSCLC and decrease the toxicity of combination chemotherapy, the search for novel drugs remains vigorous. Ent‑11α‑hydroxy‑15‑oxo‑kaur‑16‑en‑19‑oic acid (5F), a bioactive compound isolated from the herb Pteris semipinnata L., has previously been shown to induce apoptosis and inhibit proliferation in various cancer cells. One outstanding property of 5F is its minimal side effects. In the present study, 5F was combined with cisplatin to treat NCI‑H23 cells; proliferation, apoptosis and cell cycle arrest were measured by an MTT assay, Annexin V staining/flow cytometry and propidium iodide staining/flow cytometry, respectively. The messenger RNA levels of β‑catenin, glycogen synthase kinase (GSK)‑3β, c‑Myc and cyclin D1 were determined by reverse transcription‑quantitative polymerase chain reaction, and the protein levels of β‑catenin and GSK‑3β were measured by western blot analysis. The results revealed that 5F and cisplatin synergistically induced apoptosis and inhibited cell growth, arrested cell cycles in the G0/G1 phase, downregulated β‑catenin, c‑Myc and cyclin D1, and upregulated GSK‑3β. These findings merit in vivo studies using animal models of NSCLC to confirm the addition of 5F as a third drug to cisplatin‑based combination therapy for late‑stage NSCLC.
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View References

1 

Boolell V, Alamgeer M, Watkins DN and Ganju V: The evolution of therapies in non-small cell lung cancer. Cancers (Basel). 7:1815–1846. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Ramalingam S and Belani C: Systemic chemotherapy for advanced non-small cell lung cancer: Recent advances and future directions. Oncologist. 13 Suppl 1:S5–S13. 2008. View Article : Google Scholar

3 

Schiller JH, Harrington D, Belani CP, Langer C, Sandler A, Krook J, Zhu J and Johnson DH: Eastern Cooperative Oncology Group: Comparison of four chemotherapy regimens for advanced non-small-cell lung cancer. N Engl J Med. 346:92–98. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Cullen MH, Billingham LJ, Woodroffe CM, Chetiyawardana AD, Gower NH, Joshi R, Ferry DR, Rudd RM, Spiro SG, Cook JE, et al: Mitomycin, ifosfamide, and cisplatin in unresectable non-small-cell lung cancer: Effects on survival and quality of life. J Clin Oncol. 17:3188–3194. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Billingham LJ and Cullen MH: The benefits of chemotherapy in patient subgroups with unresectable non-small-cell lung cancer. Ann Oncol. 12:1671–1675. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Creagan ET, Woods JE, Schutt AJ and O'Fallon JR: Cyclophosphamide, adriamycin, and cis-diamminedichloroplatinum (II) in the treatment of advanced nonsquamous cell head and neck cancer. Cancer. 52:2007–2010. 1983. View Article : Google Scholar : PubMed/NCBI

7 

Colombo N, Sessa C, Landoni F, Sartori E, Pecorelli S and Mangioni C: Cisplatin, vinblastine, and bleomycin combination chemotherapy in metastatic granulosa cell tumor of the ovary. Obstet Gynecol. 67:265–268. 1986. View Article : Google Scholar : PubMed/NCBI

8 

Decatris MP, Sundar S and O'Byrne KJ: Platinum-based chemotherapy in metastatic breast cancer: Current status. Cancer Treat Rev. 30:53–81. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Dasari S and Tchounwou PB: Cisplatin in cancer therapy: Molecular mechanisms of action. Eur J Pharmacol. 740:364–378. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Okamoto I, Miyazaki M, Morinaga R, Kaneda H, Ueda S, Hasegawa Y, Satoh T, Kawada A, Fukuoka M, Fukino K, et al: Phase I clinical and pharmacokinetic study of sorafenib in combination with carboplatin and paclitaxel in patients with advanced non-small cell lung cancer. Invest New Drugs. 28:844–853. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Han JY, Nam BH, Kim HY, Yoon SJ, Kim HT and Lee JS: A randomized phase II study of irinotecan plus cisplatin versus irinotecan plus capecitabine with or without isosorbide-5-mononitrate in advanced non-small-cell lung cancer. Ann Oncol. 23:2925–2930. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Paz-Ares L, Mezger J, Ciuleanu TE, Fischer JR, von Pawel J, Provencio M, Kazarnowicz A, Losonczy G, de Castro G Jr, Szczesna A, et al: Necitumumab plus pemetrexed and cisplatin as first-line therapy in patients with stage IV non-squamous non-small-cell lung cancer (INSPIRE): An open-label, randomised, controlled phase 3 study. Lancet Oncol. 16:328–337. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Li MY, Leung J, Kong AW, Liang NC, Wu K, Hsin MK, Deng YF, Gong X, Lv Y, Mok TS, et al: Anticancer efficacy of 5F in NNK-induced lung cancer development of A/J mice and human lung cancer cells. J Mol Med (Berl). 88:1265–1276. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Li MY, Liang NC and Chen GG: Ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid induces apoptosis of human malignant cancer cells. Curr Drug Targets. 13:1730–1737. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Liu ZM, Chen GG, Vlantis AC, Liang NC, Deng YF and van Hasselt CA: Cell death induced by ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid in anaplastic thyroid carcinoma cells is via a mitochondrial-mediated pathway. Apoptosis. 10:1345–1356. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Wu K, Liu Y, Lv Y, Cui L, Li W, Chen J, Liang NC and Li L: Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid induces apoptosis and cell cycle arrest in CNE-2Z nasopharyngeal carcinoma cells. Oncol Rep. 29:2101–2108. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Ye H, Wu Q, Guo M, Wu K, Lv Y, Yu F, Liu Y, Gao X, Zhu Y, Cui L, et al: Growth inhibition effects of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid on colorectal carcinoma cells and colon carcinoma-bearing mice. Mol Med Rep. 13:3525–3532. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Chen GG, Leung J, Liang NC, Li L, Wu K, Chan UP, Leung BC, Li M, Du J, Deng YF, et al: Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid inhibits hepatocellular carcinoma in vitro and in vivo via stabilizing IkBα. Invest New Drugs. 30:2210–2218. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Lu Y, Wu K, Liang N and Chen GG: LC method for quantification of ent-11α-Hydroxy-15-oxo-kaur-16-en-19-oic acid in rabbit plasma: Validation and application to a pharmacokinetic study. Chromatographia. 70:15992009. View Article : Google Scholar

20 

Alamgeer M, Ganju V and Watkins DN: Novel therapeutic targets in non-small cell lung cancer. Curr Opin Pharmacol. 13:394–401. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Akiri G, Cherian MM, Vijayakumar S, Liu G, Bafico A and Aaronson SA: Wnt pathway aberrations including autocrine Wnt activation occur at high frequency in human non-small-cell lung carcinoma. Oncogene. 28:2163–2172. 2009. View Article : Google Scholar : PubMed/NCBI

22 

de Castria TB, da Silva EM, Gois AF and Riera R: Cisplatin versus carboplatin in combination with third-generation drugs for advanced non-small cell lung cancer. Cochrane Database Syst Rev: CD009256. 2013.doi: 10.1002/14651858.CD009256.pub2. View Article : Google Scholar

23 

Byun JM, Jeong DH, Lee DS, Kim JR, Park SG, Kang MS, Kim YN, Lee KB, Sung MS and Kim KT: Tetraarsenic oxide and cisplatin induce apoptotic synergism in cervical cancer. Oncol Rep. 29:1540–1546. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Nusse R and Varmus HE: Wnt genes. Cell. 69:1073–1087. 1992. View Article : Google Scholar : PubMed/NCBI

25 

Gao C and Chen YG: Dishevelled: The hub of Wnt signaling. Cell Signal. 22:717–727. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Wong HC, Bourdelas A, Krauss A, Lee HJ, Shao Y, Wu D, Mlodzik M, Shi DL and Zheng J: Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled. Mol Cell. 12:1251–1260. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Gordon MD and Nusse R: Wnt signaling: Multiple pathways, multiple receptors, and multiple transcription factors. J Biol Chem. 281:22429–22433. 2006. View Article : Google Scholar : PubMed/NCBI

28 

MacDonald BT, Tamai K and He X: Wnt/beta-catenin signaling: Components, mechanisms, and diseases. Dev Cell. 17:9–26. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Alao JP: The regulation of cyclin D1 degradation: Roles in cancer development and the potential for therapeutic invention. Mol Cancer. 6:242007. View Article : Google Scholar : PubMed/NCBI

30 

Diehl JA: Cycling to cancer with cyclin D1. Cancer Biol Ther. 1:226–231. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Meyer N and Penn LZ: Reflecting on 25 years with MYC. Nat Rev Cancer. 8:976–990. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Nesbit CE, Tersak JM and Prochownik EV: MYC oncogenes and human neoplastic disease. Oncogene. 18:3004–3016. 1999. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Li W, Deng W, Gan Y, Wu K and Sun J: Synergistic anti‑proliferative and pro‑apoptotic activities of 5F and cisplatin in human non‑small cell lung cancer NCI‑H23 cells. Oncol Lett 14: 5347-5353, 2017.
APA
Li, Y., Li, W., Deng, W., Gan, Y., Wu, K., & Sun, J. (2017). Synergistic anti‑proliferative and pro‑apoptotic activities of 5F and cisplatin in human non‑small cell lung cancer NCI‑H23 cells. Oncology Letters, 14, 5347-5353. https://doi.org/10.3892/ol.2017.6848
MLA
Li, Y., Li, W., Deng, W., Gan, Y., Wu, K., Sun, J."Synergistic anti‑proliferative and pro‑apoptotic activities of 5F and cisplatin in human non‑small cell lung cancer NCI‑H23 cells". Oncology Letters 14.5 (2017): 5347-5353.
Chicago
Li, Y., Li, W., Deng, W., Gan, Y., Wu, K., Sun, J."Synergistic anti‑proliferative and pro‑apoptotic activities of 5F and cisplatin in human non‑small cell lung cancer NCI‑H23 cells". Oncology Letters 14, no. 5 (2017): 5347-5353. https://doi.org/10.3892/ol.2017.6848
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Li W, Deng W, Gan Y, Wu K and Sun J: Synergistic anti‑proliferative and pro‑apoptotic activities of 5F and cisplatin in human non‑small cell lung cancer NCI‑H23 cells. Oncol Lett 14: 5347-5353, 2017.
APA
Li, Y., Li, W., Deng, W., Gan, Y., Wu, K., & Sun, J. (2017). Synergistic anti‑proliferative and pro‑apoptotic activities of 5F and cisplatin in human non‑small cell lung cancer NCI‑H23 cells. Oncology Letters, 14, 5347-5353. https://doi.org/10.3892/ol.2017.6848
MLA
Li, Y., Li, W., Deng, W., Gan, Y., Wu, K., Sun, J."Synergistic anti‑proliferative and pro‑apoptotic activities of 5F and cisplatin in human non‑small cell lung cancer NCI‑H23 cells". Oncology Letters 14.5 (2017): 5347-5353.
Chicago
Li, Y., Li, W., Deng, W., Gan, Y., Wu, K., Sun, J."Synergistic anti‑proliferative and pro‑apoptotic activities of 5F and cisplatin in human non‑small cell lung cancer NCI‑H23 cells". Oncology Letters 14, no. 5 (2017): 5347-5353. https://doi.org/10.3892/ol.2017.6848
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