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Endocan silencing induces programmed cell death in hepatocarcinoma

  • Authors:
    • Jinghui Yang
    • Shihou Sheng
    • Qiwei Yang
    • Li Li
    • Shaoyou Qin
    • Shan Yu
    • Xuewen Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatopancreatobiliary Surgery, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China, Department of Colorectal & Anal Surgery, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China, Central Laboratory, Second Hospital, Jilin University, Changchun, Jilin 130041, P.R. China, Department of Respiratory Medicine, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China, Department of Digestive Internal Medicine, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China, Department of Neurology, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5333-5339
    |
    Published online on: August 31, 2017
       https://doi.org/10.3892/ol.2017.6857
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Abstract

Hepatocarcinoma is a type of high‑grade malignant carcinoma identified worldwide. Its rapid development and late diagnosis prevents effective tumor resection in the majority of patients, and therefore recent studies have targeted metabolic signaling pathways and the tumor microenvironment for potential treatments. To investigate whether endocan may be a gene target for hepatocarcinoma treatment, the present study employed the following measures: MTT and Transwell assays, flow cytometry, western blotting and an mRFP‑GFP‑LC3 double fluorescence system. Following endocan gene silencing, cell proliferation was significantly inhibited and the number of invasive cells in the endocan siRNA‑treated group was reduced compared with the control‑siRNA treated‑group. Furthermore, the apoptosis rate was 15% and autophagy was detected in the endocan short interfering (si)RNA‑treated group compared with the control‑siRNA treated‑group. Using western blotting to detect NF‑κB expression in the nucleus, the NF‑κB expression was identified to be significantly reduced in the siRNA‑treated group compared with the control groups. Endocan gene silencing inhibited hepatocarcinoma cell viability and invasion, whilst inducing apoptosis and autophagy. The results of the present study suggest that the effect of endocan gene silencing on cell survival was mediated via the NF‑κB signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yang J, Sheng S, Yang Q, Li L, Qin S, Yu S and Zhang X: Endocan silencing induces programmed cell death in hepatocarcinoma. Oncol Lett 14: 5333-5339, 2017.
APA
Yang, J., Sheng, S., Yang, Q., Li, L., Qin, S., Yu, S., & Zhang, X. (2017). Endocan silencing induces programmed cell death in hepatocarcinoma. Oncology Letters, 14, 5333-5339. https://doi.org/10.3892/ol.2017.6857
MLA
Yang, J., Sheng, S., Yang, Q., Li, L., Qin, S., Yu, S., Zhang, X."Endocan silencing induces programmed cell death in hepatocarcinoma". Oncology Letters 14.5 (2017): 5333-5339.
Chicago
Yang, J., Sheng, S., Yang, Q., Li, L., Qin, S., Yu, S., Zhang, X."Endocan silencing induces programmed cell death in hepatocarcinoma". Oncology Letters 14, no. 5 (2017): 5333-5339. https://doi.org/10.3892/ol.2017.6857
Copy and paste a formatted citation
x
Spandidos Publications style
Yang J, Sheng S, Yang Q, Li L, Qin S, Yu S and Zhang X: Endocan silencing induces programmed cell death in hepatocarcinoma. Oncol Lett 14: 5333-5339, 2017.
APA
Yang, J., Sheng, S., Yang, Q., Li, L., Qin, S., Yu, S., & Zhang, X. (2017). Endocan silencing induces programmed cell death in hepatocarcinoma. Oncology Letters, 14, 5333-5339. https://doi.org/10.3892/ol.2017.6857
MLA
Yang, J., Sheng, S., Yang, Q., Li, L., Qin, S., Yu, S., Zhang, X."Endocan silencing induces programmed cell death in hepatocarcinoma". Oncology Letters 14.5 (2017): 5333-5339.
Chicago
Yang, J., Sheng, S., Yang, Q., Li, L., Qin, S., Yu, S., Zhang, X."Endocan silencing induces programmed cell death in hepatocarcinoma". Oncology Letters 14, no. 5 (2017): 5333-5339. https://doi.org/10.3892/ol.2017.6857
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