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MicroRNA‑10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway

  • Authors:
    • Kongliang Ke
    • Tingting Lou
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Ningbo First Hospital, Ningbo, Zhejiang 315010, P.R. China, Department of Breast Surgery, Ningbo Hangzhou Bay Hospital, Ningbo, Zhejiang 315336, P.R. China
    Copyright: © Ke et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5994-6000
    |
    Published online on: September 14, 2017
       https://doi.org/10.3892/ol.2017.6930
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Abstract

Previous studies have demonstrated that microRNA‑10a (miR‑10a) regulates various opposing biological functions in breast cancer. The aim of the present study was to investigate the exact functions of miR‑10a in the pathogenesis of breast cancer. miR‑10a expression was initially detected in two human breast cancer cell lines, MCF‑7 and MDA‑MB‑231 and a normal human mammary epithelial cell line MCF‑10A. The proliferation, migration and apoptosis of breast cancer cells were analyzed using MTT assays, Transwell assays and flow cytometry, respectively, following transfection of MCF‑7 and MDA‑MB‑231 cells with an miR‑10a mimic or anti‑miR‑10a. The expression of phosphorylated (p‑)protein kinase B (Akt), p‑mammalian target of rapamycin (p‑mTOR), p‑ribosomal protein S6 kinase β‑1 (p‑p70S6K), phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit α (PIK3CA), Cytochrome C (Cyt C), B‑cell lymphoma 2 (Bcl‑2), BCL‑2 associated X, apoptosis regulator (Bax), and cleaved caspase‑3 were analyzed by western blotting. The migration of MCF‑7 cells pretreated with an mTOR inhibitor CCI‑779, was detected using a Transwell assay. Relative miR‑10a expression was significantly elevated in MDA‑MB‑231 breast cancer cells and was at its highest levels in MCF‑7 cells. Transfection with the miR‑10a mimic significantly inhibited proliferation and migration, and promoted the apoptosis of breast cancer cells. Furthermore, upregulation of miR‑10a markedly suppressed the levels of p‑Akt, p‑mTOR, p‑p70S6K, and PIK3CA, and increased the expression of Cyt C, cleaved caspase‑3, and the ratio of Bax/Bcl‑2. Anti‑miR‑10a had the opposite effects. In addition, CCI‑779 reversed the effect of anti‑miR‑10a on the migration of MCF‑7 cells in a dose‑dependent manner. In conclusion, miR‑10a is downregulated in high aggressive breast cancer cells. miR‑10a inhibited the proliferation and migration, and promoted apoptosis of breast cancer cells via phosphoinositide/Akt/mTOR signaling, and the mitochondrial apoptotic pathway.
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1 

Lauby-Secretan B, Scoccianti C, Loomis D, Benbrahim-Tallaa L, Bouvard V, Bianchini F and Straif K: International Agency for Research on Cancer Handbook Working Group: Breast-cancer screening-viewpoint of the IARC Working Group. N Engl J Med. 372:2353–2358. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Lu K, Wang J, Song Y, Zhao S, Liu H, Tang D, Pan B, Zhao H and Zhang Q: miRNA-24-3p promotes cell proliferation and inhibits apoptosis in human breast cancer by targeting p27Kip1. Oncol Rep. 34:995–1002. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Shi W, Bruce J, Lee M, Yue S, Rowe M, Pintilie M, Kogo R, Bissey PA, Fyles A, Yip KW and Liu FF: MiR-449a promotes breast cancer progression by targeting CRIP2. Oncotarget. 7:18906–18918. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Lin Z, Li JW, Wang Y, Chen T, Ren N, Yang L, Xu W, He H, Jiang Y, Chen X, et al: Abnormal miRNA-30e Expression is Associated with Breast Cancer Progression. Clin Lab. 62:121–128. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Zhang HD, Sun DW, Mao L, Zhang J, Jiang LH, Li J, Wu Y, Ji H, Chen W, Wang J, et al: MiR-139-5p inhibits the biological function of breast cancer cells by targeting Notch1 and mediates chemosensitivity to docetaxel. Biochem Biophys Res Commun. 465:702–713. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Long J, Ou C, Xia H, Zhu Y and Liu D: MiR-503 inhibited cell proliferation of human breast cancer cells by suppressing CCND1 expression. Tumour Biol. 36:8697–8702. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Hoppe R, Achinger-Kawecka J, Winter S, Fritz P, Lo WY, Schroth W and Brauch H: Increased expression of miR-126 and miR-10a predict prolonged relapse-free time of primary oestrogen receptor-positive breast cancer following tamoxifen treatment. Eur J Cancer. 49:3598–3608. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Pérez-Rivas LG, Jerez JM, Carmona R, de Luque V, Vicioso L, Claros MG, Viguera E, Pajares B, Sánchez A, Ribelles N, et al: A microRNA signature associated with early recurrence in breast cancer. PLoS One. 9:e918842014. View Article : Google Scholar : PubMed/NCBI

10 

Khan S, Wall D, Curran C, Newell J, Kerin MJ and Dwyer RM: MicroRNA-10a is reduced in breast cancer and regulated in part through retinoic acid. BMC Cancer. 15:3452015. View Article : Google Scholar : PubMed/NCBI

11 

Chang CH, Fan TC, Yu JC, Liao GS, Lin YC, Shih AC, Li WH and Yu AL: The prognostic significance of RUNX2 and miR-10a/10b and their inter-relationship in breast cancer. J Transl Med. 12:2572014. View Article : Google Scholar : PubMed/NCBI

12 

Pogribny IP, Filkowski JN, Tryndyak VP, Golubov A, Shpyleva SI and Kovalchuk O: Alterations of microRNAs and their targets are associated with acquired resistance of MCF-7 breast cancer cells to cisplatin. Int J Cancer. 127:1785–1794. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Guo Y, Chang H, Li J, Xu XY, Shen L, Yu ZB and Liu WC: Thymosin alpha 1 suppresses proliferation and induces apoptosis in breast cancer cells through PTEN-mediated inhibition of PI3K/Akt/mTOR signaling pathway. Apoptosis. 20:1109–1121. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Hu R, Pan W, Fedulov AV, Jester W, Jones MR, Weiss ST, Panettieri RA Jr, Tantisira K and Lu Q: MicroRNA-10a controls airway smooth muscle cell proliferation via direct targeting of the PI3Kinase pathway. FASEB J. 28:2347–2357. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Kamal A, Nayak Lakshma V, Nagesh N, Vishnuvardhan MV and Reddy Subba NV: Benzo [b]furan derivatives induces apoptosis by targeting the PI3K/Akt/mTOR signaling pathway in human breast cancer cells. Bioorg Chem. 66:124–131. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Wani ZA, Guru SK, Rao AV, Sharma S, Mahajan G, Behl A, Kumar A, Sharma PR, Kamal A, Bhushan S and Mondhe DM: A novel quinazolinone chalcone derivative induces mitochondrial dependent apoptosis and inhibits PI3K/Akt/mTOR signaling pathway in human colon cancer HCT-116 cells. Food Chem Toxicol. 87:1–11. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Jia H, Zhang Z, Zou D, Wang B, Yan Y, Luo M, Dong L, Yin H, Gong B, Li Z, et al: MicroRNA-10a is down-regulated by DNA methylation and functions as a tumor suppressor in gastric cancer cells. PLoS One. 9:e880572014. View Article : Google Scholar : PubMed/NCBI

19 

Renner O, Blanco-Aparicio C, Grassow M, Canamero M, Leal JF and Carnero A: Activation of phosphatidylinositol 3-kinase by membrane localization of p110alpha predisposes mammary glands to neoplastic transformation. Cancer Res. 68:9643–9653. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Egeland NG, Lunde S, Jonsdottir K, Lende TH, Cronin-Fenton D, Gilje B, Janssen EA and Søiland H: The role of MicroRNAs as predictors of response to tamoxifen treatment in breast cancer patients. Int J Mol Sci. 16:24243–24275. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Yang SX, Polley E and Lipkowitz S: New insights on PI3K/AKT pathway alterations and clinical outcomes in breast cancer. Cancer Treat Rev. 45:87–96. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Suen DF, Norris KL and Youle RJ: Mitochondrial dynamics and apoptosis. Genes Dev. 22:1577–1590. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Breckenridge DG and Xue D: Regulation of mitochondrial membrane permeabilization by BCL-2 family proteins and caspases. Curr Opin Cell Biol. 16:647–652. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ke K and Lou T: MicroRNA‑10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway. Oncol Lett 14: 5994-6000, 2017.
APA
Ke, K., & Lou, T. (2017). MicroRNA‑10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway. Oncology Letters, 14, 5994-6000. https://doi.org/10.3892/ol.2017.6930
MLA
Ke, K., Lou, T."MicroRNA‑10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway". Oncology Letters 14.5 (2017): 5994-6000.
Chicago
Ke, K., Lou, T."MicroRNA‑10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway". Oncology Letters 14, no. 5 (2017): 5994-6000. https://doi.org/10.3892/ol.2017.6930
Copy and paste a formatted citation
x
Spandidos Publications style
Ke K and Lou T: MicroRNA‑10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway. Oncol Lett 14: 5994-6000, 2017.
APA
Ke, K., & Lou, T. (2017). MicroRNA‑10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway. Oncology Letters, 14, 5994-6000. https://doi.org/10.3892/ol.2017.6930
MLA
Ke, K., Lou, T."MicroRNA‑10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway". Oncology Letters 14.5 (2017): 5994-6000.
Chicago
Ke, K., Lou, T."MicroRNA‑10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway". Oncology Letters 14, no. 5 (2017): 5994-6000. https://doi.org/10.3892/ol.2017.6930
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