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Article

Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer

  • Authors:
    • Yi Jin
    • Jun He
    • Jing Du
    • Ru‑Xuan Zhang
    • Hai‑Bo Yao
    • Qin‑Shu Shao
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Children's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310052, P.R. China, Department of Gastroenterology and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Department of Gastroenterology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, P.R. China
  • Pages: 6191-6197
    |
    Published online on: September 14, 2017
       https://doi.org/10.3892/ol.2017.6944
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Abstract

The purpose of the present study was to investigate the clinical significance of the expression of heparan sulfate 6‑O‑sulfotransferase 2 (HS6ST2) in gastric cancer (GC). The Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array (Affymetrix; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was used to identify differentially expressed genes in GC tissues vs. adjacent non‑tumor gastric tissues. Candidate genes were further verified by reverse transcription quantitative polymerase chain reaction (RT‑qPCR) and immunohistochemistry (IHC). In addition, an independent dataset was obtained from the Gene Expression Omnibus, and a survival analysis was performed. Microarray analysis demonstrated that HS6ST2 was upregulated (>12‑fold) in GC tissues compared with that in adjacent non‑tumor tissues. RT‑qPCR and IHC analysis of HS6ST2 in GC tissues and adjacent non‑tumor tissues confirmed the microarray data. Furthermore, a positive association was demonstrated between HS6ST2 overexpression with the depth of tumor invasion, distant metastasis, and tumor‑node metastasis stage. Survival analysis revealed an association between patients with increased expression of HS6ST2 and a poor prognosis of gastric cancer. Cox regression analysis indicated that the expression of HS6ST2 was an independent negative prognostic factor for GC. The expression of HS6ST2 in GC was significantly associated with specific clinicopathological parameters and prognosis of disease, thus we propose that HS6ST2 may represent a novel biomarker for GC.
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Copy and paste a formatted citation
Spandidos Publications style
Jin Y, He J, Du J, Zhang RX, Yao HB and Shao QS: Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer. Oncol Lett 14: 6191-6197, 2017.
APA
Jin, Y., He, J., Du, J., Zhang, R., Yao, H., & Shao, Q. (2017). Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer. Oncology Letters, 14, 6191-6197. https://doi.org/10.3892/ol.2017.6944
MLA
Jin, Y., He, J., Du, J., Zhang, R., Yao, H., Shao, Q."Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer". Oncology Letters 14.5 (2017): 6191-6197.
Chicago
Jin, Y., He, J., Du, J., Zhang, R., Yao, H., Shao, Q."Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer". Oncology Letters 14, no. 5 (2017): 6191-6197. https://doi.org/10.3892/ol.2017.6944
Copy and paste a formatted citation
x
Spandidos Publications style
Jin Y, He J, Du J, Zhang RX, Yao HB and Shao QS: Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer. Oncol Lett 14: 6191-6197, 2017.
APA
Jin, Y., He, J., Du, J., Zhang, R., Yao, H., & Shao, Q. (2017). Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer. Oncology Letters, 14, 6191-6197. https://doi.org/10.3892/ol.2017.6944
MLA
Jin, Y., He, J., Du, J., Zhang, R., Yao, H., Shao, Q."Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer". Oncology Letters 14.5 (2017): 6191-6197.
Chicago
Jin, Y., He, J., Du, J., Zhang, R., Yao, H., Shao, Q."Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer". Oncology Letters 14, no. 5 (2017): 6191-6197. https://doi.org/10.3892/ol.2017.6944
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