Open Access

Biochanin A induces anticancer effects in SK‑Mel‑28 human malignant melanoma cells via induction of apoptosis, inhibition of cell invasion and modulation of NF‑κB and MAPK signaling pathways

Retraction in: /10.3892/ol.2021.12724

  • Authors:
    • Peng Xiao
    • Bowen Zheng
    • Jiaming Sun
    • Jia Yang
  • View Affiliations

  • Published online on: September 14, 2017     https://doi.org/10.3892/ol.2017.6945
  • Pages: 5989-5993
  • Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the antitumor activity of Biochanin A in SK‑Mel‑28 human malignant melanoma cells. An MTT assay was used to study the cytotoxic effects of Biochanin A. In vitro wound healing and invasion assays were used to investigate the effects on cell migration and invasion. Fluorescence microscopy using acridine orange/propidium iodide was used to study effects on cell morphology and apoptosis. Nuclear factor (NF)‑κB and mitogen‑activated protein kinase (MAPK) protein expression levels were determined by western blot analysis. The results indicated that Biochanin A significantly inhibited the growth of SK‑Mel‑28 cells in a dose and time dependent manner. Treatment of the cells with Biochanin A induced apoptosis in a dose dependent manner. Additionally, Biochanin A led to inhibition of cell migration and invasion in a dose‑dependent manner and upregulated the expression of key proteins in the NF‑κB and MAPK signaling pathways.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Xiao P, Zheng B, Sun J and Yang J: Biochanin A induces anticancer effects in SK‑Mel‑28 human malignant melanoma cells via induction of apoptosis, inhibition of cell invasion and modulation of NF‑κB and MAPK signaling pathways Retraction in /10.3892/ol.2021.12724. Oncol Lett 14: 5989-5993, 2017
APA
Xiao, P., Zheng, B., Sun, J., & Yang, J. (2017). Biochanin A induces anticancer effects in SK‑Mel‑28 human malignant melanoma cells via induction of apoptosis, inhibition of cell invasion and modulation of NF‑κB and MAPK signaling pathways Retraction in /10.3892/ol.2021.12724. Oncology Letters, 14, 5989-5993. https://doi.org/10.3892/ol.2017.6945
MLA
Xiao, P., Zheng, B., Sun, J., Yang, J."Biochanin A induces anticancer effects in SK‑Mel‑28 human malignant melanoma cells via induction of apoptosis, inhibition of cell invasion and modulation of NF‑κB and MAPK signaling pathways Retraction in /10.3892/ol.2021.12724". Oncology Letters 14.5 (2017): 5989-5993.
Chicago
Xiao, P., Zheng, B., Sun, J., Yang, J."Biochanin A induces anticancer effects in SK‑Mel‑28 human malignant melanoma cells via induction of apoptosis, inhibition of cell invasion and modulation of NF‑κB and MAPK signaling pathways Retraction in /10.3892/ol.2021.12724". Oncology Letters 14, no. 5 (2017): 5989-5993. https://doi.org/10.3892/ol.2017.6945