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Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats

  • Authors:
    • Yuka Sugihara
    • Xiaoxu Zuo
    • Takashi Takata
    • Shengjian Jin
    • Mutumi Miyauti
    • Atusi Isikado
    • Hiromichi Imanaka
    • Masaaki Tatsuka
    • Guangying Qi
    • Fumio Shimamoto
  • View Affiliations / Copyright

    Affiliations: Department of Health Sciences, Prefectural University of Hiroshima, Minami, Hiroshima 734‑8558, Japan, Department of Pathology and Physiopathology, Guilin Medical University, Guilin, Guangxi Autonomous Region 541004, P.R. China, Department of Oral and Maxillofacial Pathobiology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima 734‑8553, Japan, R&D Division, Sunstar Inc., Osaka 569‑1195, Japan, Department of Life Sciences, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Hiroshima 727‑0023, Japan
    Copyright: © Sugihara et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5688-5694
    |
    Published online on: September 15, 2017
       https://doi.org/10.3892/ol.2017.6976
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Abstract

Bovine lactoferrin (bLF) is a multifunctional protein with anti‑inflammatory, antibacterial, antiviral, anti‑tumour and immunoregulatory effects. The present study was conducted to evaluate the anti‑inflammatory and anti‑tumour effects of liposomal bLF (LbLF) in a 1,2‑dimethylhydrazine (DMH)/dextran sulphate sodium (DSS)‑induced model of carcinogenesis in F344 rats. F344 rats were randomly divided into three groups: Control (water), 500 or 1,000 mg/kg/day LbLF; additionally, the rats were injected with DMH (20 mg/kg) once per week for 8 consecutive weeks, after one week of drinking water containing 1% DSS. All rats were sacrificed at 25 weeks. The tissues were examined for the presence of aberrant crypt foci (ACF) and subjected to histopathological analysis. Additionally, human colon cancer cells were utilised to investigate the effect of LbLF on proliferation and inflammation. Rats from the 500 and 1,000 mg/kg/day LbLF groups harboured significantly fewer colon ACF, adenomas and adenocarcinomas than the rats from the control group. Lastly, it was demonstrated that LbLF inhibits cell growth and TNF‑α mRNA expression. These data support the hypothesis that LbLF affects colorectal carcinogenesis by suppressing inflammation and cell proliferation in rats.
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Copy and paste a formatted citation
Spandidos Publications style
Sugihara Y, Zuo X, Takata T, Jin S, Miyauti M, Isikado A, Imanaka H, Tatsuka M, Qi G, Shimamoto F, Shimamoto F, et al: Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats. Oncol Lett 14: 5688-5694, 2017.
APA
Sugihara, Y., Zuo, X., Takata, T., Jin, S., Miyauti, M., Isikado, A. ... Shimamoto, F. (2017). Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats. Oncology Letters, 14, 5688-5694. https://doi.org/10.3892/ol.2017.6976
MLA
Sugihara, Y., Zuo, X., Takata, T., Jin, S., Miyauti, M., Isikado, A., Imanaka, H., Tatsuka, M., Qi, G., Shimamoto, F."Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats". Oncology Letters 14.5 (2017): 5688-5694.
Chicago
Sugihara, Y., Zuo, X., Takata, T., Jin, S., Miyauti, M., Isikado, A., Imanaka, H., Tatsuka, M., Qi, G., Shimamoto, F."Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats". Oncology Letters 14, no. 5 (2017): 5688-5694. https://doi.org/10.3892/ol.2017.6976
Copy and paste a formatted citation
x
Spandidos Publications style
Sugihara Y, Zuo X, Takata T, Jin S, Miyauti M, Isikado A, Imanaka H, Tatsuka M, Qi G, Shimamoto F, Shimamoto F, et al: Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats. Oncol Lett 14: 5688-5694, 2017.
APA
Sugihara, Y., Zuo, X., Takata, T., Jin, S., Miyauti, M., Isikado, A. ... Shimamoto, F. (2017). Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats. Oncology Letters, 14, 5688-5694. https://doi.org/10.3892/ol.2017.6976
MLA
Sugihara, Y., Zuo, X., Takata, T., Jin, S., Miyauti, M., Isikado, A., Imanaka, H., Tatsuka, M., Qi, G., Shimamoto, F."Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats". Oncology Letters 14.5 (2017): 5688-5694.
Chicago
Sugihara, Y., Zuo, X., Takata, T., Jin, S., Miyauti, M., Isikado, A., Imanaka, H., Tatsuka, M., Qi, G., Shimamoto, F."Inhibition of DMH‑DSS‑induced colorectal cancer by liposomal bovine lactoferrin in rats". Oncology Letters 14, no. 5 (2017): 5688-5694. https://doi.org/10.3892/ol.2017.6976
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