Expression of c‑Met and hepatocyte growth factor in various gastric pathologies and its association with Helicobacter pylori infection

Xie,Chuan; Yang,Zhen; Hu,Yi; Cao,Ximei; Chen,Jiang; Zhu,Yin; Lu,Nonghua

doi:10.3892/ol.2017.6983

November-2017 Volume 14 Issue 5

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November-2017 Volume 14 Issue 5

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Article

Expression of c‑Met and hepatocyte growth factor in various gastric pathologies and its association with Helicobacter pylori infection

Authors:
- Chuan Xie
- Zhen Yang
- Yi Hu
- Ximei Cao
- Jiang Chen
- Yin Zhu
- Nonghua Lu
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
Pages: 6151-6155
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Published online on: September 18, 2017

https://doi.org/10.3892/ol.2017.6983
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Abstract

Activation of the hepatocyte growth factor (HGF)/c‑Met signaling pathway was identified to be associated with malignant tumors. The present study aimed at determining the expression of HGF and c‑Met in gastric carcinogenesis and its association with Helicobacter pylori infection. Gastric biopsies were obtained from 160 H. pylori‑negative and ‑positive patients, including those with chronic gastritis, intestinal metaplasia, dysplasia and gastric cancer (GC). Proteins were extracted from GES‑1, gastric epithelial, and AGS, gastric adenocarcinoma, cells following co‑culture with H. pylori in vitro. The expression of HGF and c‑Met in gastric tissues or cells was determined using immunohistochemistry and western blot analysis. The expression of c‑Met increased in GC tissues (72.5%) compared with that in pre‑cancerous lesions (17.5, 17.5 and 30%). Additional analysis identified that the expression of HGF and c‑Met was significantly increased in the presence of H. pylori infection in dysplasia and gastric cancer samples. Furthermore, H. pylori may activate the HGF/c‑Met signaling pathway in vitro, which may contribute to gastric carcinogenesis.

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