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Nuclear paraspeckle assembly transcript 1 promotes the metastasis and epithelial‑mesenchymal transition of hepatoblastoma cells by inhibiting miR‑129‑5p

  • Authors:
    • Ming‑Cui Fu
    • Li‑Qun Yuan
    • Ting Zhang
    • Xiang‑Ming Yan
    • Yun Zhou
    • Hong‑Liang Xia
    • Yi Wu
    • Li‑Xiao Xu
    • Xu Cao
    • Jian Wang
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Children's Hospital of Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China, Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou, Jiangsu 215004, P.R. China, Department of Pathology, Children's Hospital of Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China, Institute of Pediatrics, Children's Hospital of Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China
    Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5773-5778
    |
    Published online on: September 18, 2017
       https://doi.org/10.3892/ol.2017.6995
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Abstract

The abnormal expression of nuclear paraspeckle assembly transcript 1 (NEAT1) may serve critical functions for the development and progression of various types of human tumor. However, the expression and biological function of NEAT1 in hepatoblastoma (HB) and the underlying mechanisms for the function of NEAT1 in HB remain largely uncharacterized. In the present study, the results of reverse transcription‑quantitative polymerase chain reaction revealed that the expression of NEAT1 was significantly elevated in HB tissues. HB tissues with metastasis also exhibited significantly increased levels of NEAT1 compared with tissues without metastasis. The biological functions of NEAT1 were then assessed using gain‑/loss‑of‑function studies. The results of in vitro assays revealed that inhibiting NEAT1 expression reduced the migration and invasion of HepG2 cells. By contrast, the induced expression of NEAT1 exhibited the opposite effect. The present study also demonstrated that the inhibition of NEAT1 expression prevented the epithelial‑mesenchymal transition of HepG2 cells, whereas forced expression of NEAT1 exhibited the opposite effect. In addition, it was confirmed that NEAT1 could modulate the expression of microRNA (miR)‑129‑5p in HepG2 cells, and that NEAT1 may exert its effect on the metastatic behaviors and epithelial‑mesenchymal transition of HepG2 cells by inhibiting miR‑129‑5p. In conclusion, the present study indicated that NEAT1 expression was aberrantly increased in HB and that it may promote the metastasis of HB cells by inhibiting miR‑129‑5p. Targeting NEAT1 may potentially be a novel therapeutic option for treating patients with HB.
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1 

Weinberg AG and Finegold MJ: Primary hepatic tumors of childhood. Hum Pathol. 14:512–537. 1983. View Article : Google Scholar : PubMed/NCBI

2 

Ishak KG and Glunz PR: Hepatoblastoma and hepatocarcinoma in infancy and childhood. Report of 47 cases. Cancer. 20:396–422. 1967. View Article : Google Scholar : PubMed/NCBI

3 

Czauderna P, Otte JB, Roebuck DJ, von Schweinitz D and Plaschkes J: Surgical treatment of hepatoblastoma in children. Pediatr Radiol. 36:187–191. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Haas JE, Muczynski KA, Krailo M, Ablin A, Land V, Vietti TJ and Hammond GD: Histopathology and prognosis in childhood hepatoblastoma and hepatocarcinoma. Cancer. 64:1082–1095. 1989. View Article : Google Scholar : PubMed/NCBI

5 

Mercer TR, Dinger ME and Mattick JS: Long non-coding RNAs: Insights into functions. Nat Rev Genet. 10:155–159. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Takahashi K, Yan I, Haga H and Patel T: Long noncoding RNA in liver diseases. Hepatology. 60:744–753. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Zhang H, Chen Z, Wang X, Huang Z, He Z and Chen Y: Long non-coding RNA: A new player in cancer. J Hematol Oncol. 6:372013. View Article : Google Scholar : PubMed/NCBI

8 

Prensner JR and Chinnaiyan AM: The emergence of lncRNAs in cancer biology. Cancer Discov. 1:391–407. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Tsai MC, Spitale RC and Chang HY: Long intergenic noncoding RNAs: New links in cancer progression. Cancer Res. 71:3–7. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A and Lawrence JB: An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles. Mol Cell. 33:717–726. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Souquere S, Beauclair G, Harper F, Fox A and Pierron G: Highly ordered spatial organization of the structural long noncoding NEAT1 RNAs within paraspeckle nuclear bodies. Mol Biol Cell. 21:4020–4027. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Chen X, Kong J, Ma Z, Gao S and Feng X: Up regulation of the long non-coding RNA NEAT1 promotes esophageal squamous cell carcinoma cell progression and correlates with poor prognosis. Am J Cancer Res. 5:2808–2815. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Zhen L, Yun-Hui L, Hong-Yu D, Jun M and Yi-Long Y: Long noncoding RNA NEAT1 promotes glioma pathogenesis by regulating miR-449b-5p/c-Met axis. Tumour Biol. 37:673–683. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Li Y, Li Y, Chen W, He F, Tan Z, Zheng J, Wang W, Zhao Q and Li J: NEAT expression is associated with tumor recurrence and unfavorable prognosis in colorectal cancer. Oncotarget. 6:27641–27650. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Chakravarty D, Sboner A, Nair SS, Giannopoulou E, Li R, Hennig S, Mosquera JM, Pauwels J, Park K, Kossai M, et al: The oestrogen receptor alpha-regulated lncRNA NEAT1 is a critical modulator of prostate cancer. Nat Commun. 5:53832014. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Yilmaz M and Christofori G: EMT, the cytoskeleton, and cancer cell invasion. Cancer Metastasis Rev. 28:15–33. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Jalali S, Bhartiya D, Lalwani MK, Sivasubbu S and Scaria V: Systematic transcriptome wide analysis of lncRNA-miRNA interactions. PLoS One. 8:e538232013. View Article : Google Scholar : PubMed/NCBI

19 

Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H and Liu M: Down-regulation of miR-129-5p inhibits growth and induces apoptosis in laryngeal squamous cell carcinoma by targeting APC. PLoS One. 8:e778292013. View Article : Google Scholar : PubMed/NCBI

20 

Liu Y, Hei Y, Shu Q, Dong J, Gao Y, Fu H, Zheng X and Yang G: VCP/p97, down-regulated by microRNA-129-5p, could regulate the progression of hepatocellular carcinoma. PLoS One. 7:e358002012. View Article : Google Scholar : PubMed/NCBI

21 

Duan L, Hao X, Liu Z, Zhang Y and Zhang G: miR-129-5p is down-regulated and involved in the growth, apoptosis and migration of medullary thyroid carcinoma cells through targeting RET. FEBS Lett. 588:1644–1651. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Salmena L, Poliseno L, Tay Y, Kats L and Pandolfi PP: A ceRNA hypothesis: The rosetta stone of a hidden RNA language? Cell. 146:353–358. 2011. View Article : Google Scholar : PubMed/NCBI

23 

You J, Zhang Y, Liu B, Li Y, Fang N, Zu L, Li X and Zhou Q: MicroRNA-449a inhibits cell growth in lung cancer and regulates long noncoding RNA nuclear enriched abundant transcript. Indian J Cancer. 51 Suppl 3:e77–e81. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Choudhry H, Albukhari A, Morotti M, Haider S, Moralli D, Smythies J, Schödel J, Green CM, Camps C, Buffa F, et al: Tumor hypoxia induces nuclear paraspeckle formation through HIF-2α dependent transcriptional activation of NEAT1 leading to cancer cell survival. Oncogene. 34:4482–4490. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Sumazin P, Yang X, Chiu HS, Chung WJ, Iyer A, Llobet-Navas D, Rajbhandari P, Bansal M, Guarnieri P, Silva J and Califano A: An extensive microRNA-mediated network of RNA-RNA interactions regulates established oncogenic pathways in glioblastoma. Cell. 147:370–381. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Deng L, Yang SB, Xu FF and Zhang JH: Long noncoding RNA CCAT1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge. J Exp Clin Cancer Res. 34:182015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fu MC, Yuan LQ, Zhang T, Yan XM, Zhou Y, Xia HL, Wu Y, Xu LX, Cao X, Wang J, Wang J, et al: Nuclear paraspeckle assembly transcript 1 promotes the metastasis and epithelial‑mesenchymal transition of hepatoblastoma cells by inhibiting miR‑129‑5p. Oncol Lett 14: 5773-5778, 2017.
APA
Fu, M., Yuan, L., Zhang, T., Yan, X., Zhou, Y., Xia, H. ... Wang, J. (2017). Nuclear paraspeckle assembly transcript 1 promotes the metastasis and epithelial‑mesenchymal transition of hepatoblastoma cells by inhibiting miR‑129‑5p. Oncology Letters, 14, 5773-5778. https://doi.org/10.3892/ol.2017.6995
MLA
Fu, M., Yuan, L., Zhang, T., Yan, X., Zhou, Y., Xia, H., Wu, Y., Xu, L., Cao, X., Wang, J."Nuclear paraspeckle assembly transcript 1 promotes the metastasis and epithelial‑mesenchymal transition of hepatoblastoma cells by inhibiting miR‑129‑5p". Oncology Letters 14.5 (2017): 5773-5778.
Chicago
Fu, M., Yuan, L., Zhang, T., Yan, X., Zhou, Y., Xia, H., Wu, Y., Xu, L., Cao, X., Wang, J."Nuclear paraspeckle assembly transcript 1 promotes the metastasis and epithelial‑mesenchymal transition of hepatoblastoma cells by inhibiting miR‑129‑5p". Oncology Letters 14, no. 5 (2017): 5773-5778. https://doi.org/10.3892/ol.2017.6995
Copy and paste a formatted citation
x
Spandidos Publications style
Fu MC, Yuan LQ, Zhang T, Yan XM, Zhou Y, Xia HL, Wu Y, Xu LX, Cao X, Wang J, Wang J, et al: Nuclear paraspeckle assembly transcript 1 promotes the metastasis and epithelial‑mesenchymal transition of hepatoblastoma cells by inhibiting miR‑129‑5p. Oncol Lett 14: 5773-5778, 2017.
APA
Fu, M., Yuan, L., Zhang, T., Yan, X., Zhou, Y., Xia, H. ... Wang, J. (2017). Nuclear paraspeckle assembly transcript 1 promotes the metastasis and epithelial‑mesenchymal transition of hepatoblastoma cells by inhibiting miR‑129‑5p. Oncology Letters, 14, 5773-5778. https://doi.org/10.3892/ol.2017.6995
MLA
Fu, M., Yuan, L., Zhang, T., Yan, X., Zhou, Y., Xia, H., Wu, Y., Xu, L., Cao, X., Wang, J."Nuclear paraspeckle assembly transcript 1 promotes the metastasis and epithelial‑mesenchymal transition of hepatoblastoma cells by inhibiting miR‑129‑5p". Oncology Letters 14.5 (2017): 5773-5778.
Chicago
Fu, M., Yuan, L., Zhang, T., Yan, X., Zhou, Y., Xia, H., Wu, Y., Xu, L., Cao, X., Wang, J."Nuclear paraspeckle assembly transcript 1 promotes the metastasis and epithelial‑mesenchymal transition of hepatoblastoma cells by inhibiting miR‑129‑5p". Oncology Letters 14, no. 5 (2017): 5773-5778. https://doi.org/10.3892/ol.2017.6995
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