Early changes in the apparent diffusion coefficient and MMP‑9 expression of a cervical carcinoma U14 allograft model following irradiation

Huang,Yecai; Huang,Jianming; Feng,Mei; Ren,Jing; Mi,Kun; Cheng,Jia; Song,Bing; Lang,Jinyi

doi:10.3892/ol.2017.7035

Early changes in the apparent diffusion coefficient and MMP‑9 expression of a cervical carcinoma U14 allograft model following irradiation

Authors:
- Yecai Huang
- Jianming Huang
- Mei Feng
- Jing Ren
- Kun Mi
- Jia Cheng
- Bing Song
- Jinyi Lang
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Affiliations: Department of Radiation Oncology, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041, P.R. China, Department of Biochemistry and Molecular Biology, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041, P.R. China, Department of Radiology, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041, P.R. China, Department of Radiology, West China Hospital of Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: September 22, 2017 https://doi.org/10.3892/ol.2017.7035
Pages: 6769-6775

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Abstract

A cervical carcinoma allograft model was designed to assess the correlation between early changes in the apparent diffusion coefficient (ADC) values on diffusion‑weighted magnetic resonance imaging (DW‑MRI) and the expression of matrix metalloproteinase‑9 (MMP‑9) in tumors. BALB/c mice with U14 tumor allografts on the right rear flank were irradiated with a single 20 Gy dose. All tumor‑bearing mice were subjected to DW‑MRI, followed by calculation of the ADC values and characterization of the T1 and T2 relaxation time constants. Pre‑ and post‑irradiation ADC values were compared with the tumor volume, and the immunohistochemical staining of MMP‑9 and hematoxylin‑eosin (HE) staining of tumor allografts. However, no correlations between the pre‑treatment ADC values and changes in tumor volumes following irradiation were observed. Notably, the mean ADC value was significantly higher in the irradiated tumors (0.756±0.102x10‑3 mm2/sec) as compared with those in the untreated tumors (0.501±0.052x10‑3 mm2/sec; P=0.002; r=0.682). Additionally, immunohistochemical staining demonstrated that MMP‑9 expression in the irradiated tumors was significantly increased. The mean ADC value was significantly higher in the irradiated tumors with high MMP‑9 expression levels (0.815±0.112x10‑3 mm2/sec), as compared with in the untreated tumors with low MMP‑9 expression levels (0.631±0.068x10‑3 mm2/sec). Quantitative analysis determined that the ADC values were correlated with MMP‑9 expression (r=0.752; P=0.003). Combined, these results suggest that radiation‑induced increases in MMP‑9 expression levels may be responsible for early changes in the mean ADC value and the response to irradiation in cervical carcinoma.

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