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Hypermethylation downregulates P2X7 receptor expression in astrocytoma

  • Authors:
    • Jing Liu
    • Ningning Li
    • Ruofan Sheng
    • Rui Wang
    • Zude Xu
    • Ying Mao
    • Yin Wang
    • Ying Liu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, School of Basic Medical Science, Fudan University, Shanghai 200032, P.R. China, Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Neuropathology, Institute of Neurology of Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7699-7704
    |
    Published online on: October 20, 2017
       https://doi.org/10.3892/ol.2017.7241
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Abstract

The present study investigated the altered expression of p2X purinoceptor (P2X7R) in astrocytoma. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to determine the P2X7R expression in glioblastoma (GBM) and surrounding normal brain tissue. DNA methylation levels of P2X7R gene promoter in GBM were analyzed using a Sequenom MassARRAY® System. Immunohistochemistry (IHC) was used to detect the expression of P2X7R in astrocytoma at different malignancy grades, including diffuse astrocytoma, anaplastic astrocytoma and GBM. P2X7R mRNA and protein were significantly decreased in GBM compared with normal brain tissues. IHC results showed a negative correlation between P2X7R expression and tumor grade. The decreased P2X7R expression was mostly attributed to hypermethylation of its promoter. Therefore, P2X7R was found to perform an important role in tumorigenesis and progression of astrocytoma.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Li N, Sheng R, Wang R, Xu Z, Mao Y, Wang Y and Liu Y: Hypermethylation downregulates P2X7 receptor expression in astrocytoma. Oncol Lett 14: 7699-7704, 2017.
APA
Liu, J., Li, N., Sheng, R., Wang, R., Xu, Z., Mao, Y. ... Liu, Y. (2017). Hypermethylation downregulates P2X7 receptor expression in astrocytoma. Oncology Letters, 14, 7699-7704. https://doi.org/10.3892/ol.2017.7241
MLA
Liu, J., Li, N., Sheng, R., Wang, R., Xu, Z., Mao, Y., Wang, Y., Liu, Y."Hypermethylation downregulates P2X7 receptor expression in astrocytoma". Oncology Letters 14.6 (2017): 7699-7704.
Chicago
Liu, J., Li, N., Sheng, R., Wang, R., Xu, Z., Mao, Y., Wang, Y., Liu, Y."Hypermethylation downregulates P2X7 receptor expression in astrocytoma". Oncology Letters 14, no. 6 (2017): 7699-7704. https://doi.org/10.3892/ol.2017.7241
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Li N, Sheng R, Wang R, Xu Z, Mao Y, Wang Y and Liu Y: Hypermethylation downregulates P2X7 receptor expression in astrocytoma. Oncol Lett 14: 7699-7704, 2017.
APA
Liu, J., Li, N., Sheng, R., Wang, R., Xu, Z., Mao, Y. ... Liu, Y. (2017). Hypermethylation downregulates P2X7 receptor expression in astrocytoma. Oncology Letters, 14, 7699-7704. https://doi.org/10.3892/ol.2017.7241
MLA
Liu, J., Li, N., Sheng, R., Wang, R., Xu, Z., Mao, Y., Wang, Y., Liu, Y."Hypermethylation downregulates P2X7 receptor expression in astrocytoma". Oncology Letters 14.6 (2017): 7699-7704.
Chicago
Liu, J., Li, N., Sheng, R., Wang, R., Xu, Z., Mao, Y., Wang, Y., Liu, Y."Hypermethylation downregulates P2X7 receptor expression in astrocytoma". Oncology Letters 14, no. 6 (2017): 7699-7704. https://doi.org/10.3892/ol.2017.7241
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