MicroRNA-26b-5p regulates cell proliferation, invasion and metastasis in human intrahepatic cholangiocarcinoma by targeting S100A7

Fan,Fei; Lu,Jiongjiong; Yu,Wenlong; Zhang,Yongjie; Xu,Suqian; Pang,Leilei; Zhu,Bin

doi:10.3892/ol.2017.7331

MicroRNA-26b-5p regulates cell proliferation, invasion and metastasis in human intrahepatic cholangiocarcinoma by targeting S100A7

Authors:
- Fei Fan
- Jiongjiong Lu
- Wenlong Yu
- Yongjie Zhang
- Suqian Xu
- Leilei Pang
- Bin Zhu
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Affiliations: Department of Special Treatment, Eastern Hepatobiliary Surgical Hospital, Second Military Medical University, Shanghai 200438, P.R. China, Department of Biliary Surgery II, Eastern Hepatobiliary Surgical Hospital, Second Military Medical University, Shanghai 200438, P.R. China, Department of Pathology, Maternal and Child Health Hospital of Weihai, Weihai, Shandong 264200, P.R. China
Published online on: November 2, 2017 https://doi.org/10.3892/ol.2017.7331
Pages: 386-392

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Abstract

The present study aimed to investigate the effects of microRNA expression in intrahepatic cholangiocarcinoma (ICC). It was identified that the expression of microRNA‑26b‑5p (miR‑26b‑5p) was downregulated in ICC tissues compared with matched adjacent non‑tumor tissues. Furthermore, miR‑26b‑5p expression was downregulated in metastatic ICC tumor tissues and invasive ICC cell line subpopulations compared with non‑metastatic tumor tissue and the parental ICC cells. In vitro studies demonstrated that transfection with an miR‑26b‑5p mimic inhibited the proliferation, migration and invasion of RBE and HCCC‑9810 cells, whereas an miR‑26b‑5p inhibitor promoted these abilities. S100 calcium‑binding protein A7 (S100A7) was predicted as a direct target of miR‑26b‑5p. Transfection with an miR‑26b‑5p mimic decreased S100A7 expression, whereas an miR‑26b‑5p inhibitor increased S100A7 expression. The result of a dual luciferase reporter assay also indicated this interaction. S100A7 was therefore confirmed as a direct target of miR‑26b‑5p in ICC. The knockdown of S100A7 abrogated the effect of miR‑26b‑5p on cell migration in RBE and HCCC‑9810 cells. In conclusion, the present study demonstrated that miR‑26b‑5p suppresses the proliferation, migration and invasion of ICC cells by suppressing S100A7.

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