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Characterization of the T‑cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer

  • Authors:
    • Song Liu
    • Weibing Pan
    • Zhiqiang Cheng
    • Guoping Sun
    • Peng Zhu
    • Franky Chan
    • Yunlong Hu
    • Xinzhou Zhang
    • Yong Dai
  • View Affiliations / Copyright

    Affiliations: Clinical Medical Research Center, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, P.R. China, Urology Division of Shenzhen Pingshan People's Hospital, Shenzhen, Guangdong 518118, P.R. China, Department of Pathology, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, P.R. China, Central Lab of Shenzhen Pingshan People's Hospital, Shenzhen, Guangdong 518118, P.R. China, Cancer Biology and Experimental Therapeutics, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China, Cancer Research Center, Shenzhen University School of Medicine, Shenzhen, Guangdong 518000, P.R. China, Department of Nephrology, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong 518020, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1744-1752
    |
    Published online on: November 23, 2017
       https://doi.org/10.3892/ol.2017.7479
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Abstract

Prostate cancer (PC) is the most prevalent urological cancer in men. T cells serve a central role in the cancer's immunological microenvironment. In the present study, we applied multiplex PCR and Illumina next‑generation sequencing to study the clonal diversity of the T‑cell receptor (TCR) repertoire in cancer tissues and paracancer tissues from patients with PC. It was found that the TCR repertoire in the PC samples had a notably more skewed clonotype composition, with a greater number of highly expanded clones (HECs) compared with the prostate paracancer samples. The amino acid sequences ATSRVAGETQY (1.008 vs. 0.002%), ATSRTGRWETQY (3.985 vs. 0.007%), ATSDSSDYEQY (12.464 vs. 0.027%), ATSDFRGQPQETQY (2.205 vs. 0.06%), ASSQQDEAF (1.109 vs. 0.002%) and ARPTRTEETQY (1.263 vs. 0.002%) were found to vary markedly between cancer and paracancer tissues, respectively. In conclusion, the present study identified PC‑specific HECs, which are critical to improving understanding of the TCR repertoire in PC. This may accelerate the screening process for potential new autoantigens and provide information for generating more effective T cell‑targeted diagnostic and therapeutic strategies.
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Copy and paste a formatted citation
Spandidos Publications style
Liu S, Pan W, Cheng Z, Sun G, Zhu P, Chan F, Hu Y, Zhang X and Dai Y: Characterization of the T‑cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer. Oncol Lett 15: 1744-1752, 2018.
APA
Liu, S., Pan, W., Cheng, Z., Sun, G., Zhu, P., Chan, F. ... Dai, Y. (2018). Characterization of the T‑cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer. Oncology Letters, 15, 1744-1752. https://doi.org/10.3892/ol.2017.7479
MLA
Liu, S., Pan, W., Cheng, Z., Sun, G., Zhu, P., Chan, F., Hu, Y., Zhang, X., Dai, Y."Characterization of the T‑cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer". Oncology Letters 15.2 (2018): 1744-1752.
Chicago
Liu, S., Pan, W., Cheng, Z., Sun, G., Zhu, P., Chan, F., Hu, Y., Zhang, X., Dai, Y."Characterization of the T‑cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer". Oncology Letters 15, no. 2 (2018): 1744-1752. https://doi.org/10.3892/ol.2017.7479
Copy and paste a formatted citation
x
Spandidos Publications style
Liu S, Pan W, Cheng Z, Sun G, Zhu P, Chan F, Hu Y, Zhang X and Dai Y: Characterization of the T‑cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer. Oncol Lett 15: 1744-1752, 2018.
APA
Liu, S., Pan, W., Cheng, Z., Sun, G., Zhu, P., Chan, F. ... Dai, Y. (2018). Characterization of the T‑cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer. Oncology Letters, 15, 1744-1752. https://doi.org/10.3892/ol.2017.7479
MLA
Liu, S., Pan, W., Cheng, Z., Sun, G., Zhu, P., Chan, F., Hu, Y., Zhang, X., Dai, Y."Characterization of the T‑cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer". Oncology Letters 15.2 (2018): 1744-1752.
Chicago
Liu, S., Pan, W., Cheng, Z., Sun, G., Zhu, P., Chan, F., Hu, Y., Zhang, X., Dai, Y."Characterization of the T‑cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer". Oncology Letters 15, no. 2 (2018): 1744-1752. https://doi.org/10.3892/ol.2017.7479
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