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A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway

  • Authors:
    • Lin Cheng
    • Liang Shi
    • Jing Wu
    • Xujie Zhou
    • Xiaoxia Li
    • Xi Sun
    • Lei Zhu
    • Tian‑Song Xia
    • Qiang Ding
  • View Affiliations / Copyright

    Affiliations: Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1737-1743
    |
    Published online on: November 27, 2017
       https://doi.org/10.3892/ol.2017.7494
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Abstract

Natural plants offer a treasure trove of resources for anticancer drug development. Clematis are widely used in Traditional Chinese Medicine. However, studies on the active substances in Clematis are scarce. In the present study, four monomer compounds were successfully extracted from this species and their inhibitory effects on the growth of breast cancer cells were investigated using bioactivity tests. Among them, Clematis hederagenin saponin (CHS) belongs to the class of triterpenoid saponins. CHS showed cytotoxic effects on breast cancer cells in a dose‑ and time‑dependent manner. The compound also induced apoptosis in breast cancer cells in a time‑dependent manner. Further investigation into the underlying mechanisms of apoptosis induction in breast cancer cells showed that the compound significantly reduced mitochondrial Apaf‑1 and cytochrome c proteins in breast cancer cells. In addition, it upregulated the activities of caspase‑3 and ‑9. In conclusion, CHS induced apoptosis in breast cancer cells through regulation of the mitochondrial apoptosis pathway. The results suggest that the hederagenin saponin extracted from Clematis ganpiniana offers great potential as a novel anti‑breast cancer drug.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng L, Shi L, Wu J, Zhou X, Li X, Sun X, Zhu L, Xia TS and Ding Q: A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway. Oncol Lett 15: 1737-1743, 2018.
APA
Cheng, L., Shi, L., Wu, J., Zhou, X., Li, X., Sun, X. ... Ding, Q. (2018). A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway. Oncology Letters, 15, 1737-1743. https://doi.org/10.3892/ol.2017.7494
MLA
Cheng, L., Shi, L., Wu, J., Zhou, X., Li, X., Sun, X., Zhu, L., Xia, T., Ding, Q."A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway". Oncology Letters 15.2 (2018): 1737-1743.
Chicago
Cheng, L., Shi, L., Wu, J., Zhou, X., Li, X., Sun, X., Zhu, L., Xia, T., Ding, Q."A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway". Oncology Letters 15, no. 2 (2018): 1737-1743. https://doi.org/10.3892/ol.2017.7494
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng L, Shi L, Wu J, Zhou X, Li X, Sun X, Zhu L, Xia TS and Ding Q: A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway. Oncol Lett 15: 1737-1743, 2018.
APA
Cheng, L., Shi, L., Wu, J., Zhou, X., Li, X., Sun, X. ... Ding, Q. (2018). A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway. Oncology Letters, 15, 1737-1743. https://doi.org/10.3892/ol.2017.7494
MLA
Cheng, L., Shi, L., Wu, J., Zhou, X., Li, X., Sun, X., Zhu, L., Xia, T., Ding, Q."A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway". Oncology Letters 15.2 (2018): 1737-1743.
Chicago
Cheng, L., Shi, L., Wu, J., Zhou, X., Li, X., Sun, X., Zhu, L., Xia, T., Ding, Q."A hederagenin saponin isolated from Clematis ganpiniana induces apoptosis in breast cancer cells via the mitochondrial pathway". Oncology Letters 15, no. 2 (2018): 1737-1743. https://doi.org/10.3892/ol.2017.7494
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