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Article Open Access

Notch signaling via regulation of RB and p‑AKT but not PIK3CG contributes to MIA PaCa‑2 cell growth and migration to affect pancreatic carcinogenesis

  • Authors:
    • Shubing Zhang
    • Jingjiang Liu
    • Keli Xu
    • Zhijian Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Cell Biology, School of Life Sciences, Central South University, Changsha, Hunan 410011, P.R. China, Cancer Institute, University of Mississippi Medical Center, Jackson, MS 39216, USA, Department of Anesthesiology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 41001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2105-2110
    |
    Published online on: December 8, 2017
       https://doi.org/10.3892/ol.2017.7551
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Abstract

Pancreatic cancer is one of the leading causes of cancer‑associated mortality. The understanding of the expression pattern of key protein factors and their function in pancreatic cancer cells is therefore vital for the diagnosis and treatment of this malignancy. The results of the present study reveal that the levels of neurogenic locus notch homolog protein 2 (Notch2) and phosphorylated (p)‑SMAD family member 2 decreased, whereas the expression of Notch3 and phosphoinositide‑3 kinase catalytic subunit‑γ protein increased in human pancreatic cancer tissues compared with tumor‑adjacent tissues. Using the human pancreatic cancer MIA PaCa‑2 cell line, it was observed that retinoblastoma-associated protein (RB) and p‑RB expression were inhibited and p‑AKT was upregulated when Notch signaling was activated in MIA PaCa‑2 cells. Furthermore, inhibition of phosphoinositide‑3 kinase catalytic subunit‑γ (PIK3CG) activity by AS‑605240 was able to block the growth and migration of MIA PaCa‑2 cells. In conclusion, the results of the present study demonstrate that the Notch signal pathway may be involved in pancreatic carcinogenesis by modulating RB and p‑AKT. PIK3CG may therefore be a potential target gene for the treatment of pancreatic cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang S, Liu J, Xu K and Li Z: Notch signaling via regulation of RB and p‑AKT but not PIK3CG contributes to MIA PaCa‑2 cell growth and migration to affect pancreatic carcinogenesis. Oncol Lett 15: 2105-2110, 2018.
APA
Zhang, S., Liu, J., Xu, K., & Li, Z. (2018). Notch signaling via regulation of RB and p‑AKT but not PIK3CG contributes to MIA PaCa‑2 cell growth and migration to affect pancreatic carcinogenesis. Oncology Letters, 15, 2105-2110. https://doi.org/10.3892/ol.2017.7551
MLA
Zhang, S., Liu, J., Xu, K., Li, Z."Notch signaling via regulation of RB and p‑AKT but not PIK3CG contributes to MIA PaCa‑2 cell growth and migration to affect pancreatic carcinogenesis". Oncology Letters 15.2 (2018): 2105-2110.
Chicago
Zhang, S., Liu, J., Xu, K., Li, Z."Notch signaling via regulation of RB and p‑AKT but not PIK3CG contributes to MIA PaCa‑2 cell growth and migration to affect pancreatic carcinogenesis". Oncology Letters 15, no. 2 (2018): 2105-2110. https://doi.org/10.3892/ol.2017.7551
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Liu J, Xu K and Li Z: Notch signaling via regulation of RB and p‑AKT but not PIK3CG contributes to MIA PaCa‑2 cell growth and migration to affect pancreatic carcinogenesis. Oncol Lett 15: 2105-2110, 2018.
APA
Zhang, S., Liu, J., Xu, K., & Li, Z. (2018). Notch signaling via regulation of RB and p‑AKT but not PIK3CG contributes to MIA PaCa‑2 cell growth and migration to affect pancreatic carcinogenesis. Oncology Letters, 15, 2105-2110. https://doi.org/10.3892/ol.2017.7551
MLA
Zhang, S., Liu, J., Xu, K., Li, Z."Notch signaling via regulation of RB and p‑AKT but not PIK3CG contributes to MIA PaCa‑2 cell growth and migration to affect pancreatic carcinogenesis". Oncology Letters 15.2 (2018): 2105-2110.
Chicago
Zhang, S., Liu, J., Xu, K., Li, Z."Notch signaling via regulation of RB and p‑AKT but not PIK3CG contributes to MIA PaCa‑2 cell growth and migration to affect pancreatic carcinogenesis". Oncology Letters 15, no. 2 (2018): 2105-2110. https://doi.org/10.3892/ol.2017.7551
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