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Article

Augmentation of antibody-dependent cellular cytotoxicity with defucosylated monoclonal antibodies in patients with GI-tract cancer

  • Authors:
    • Takahiro Nakajima
    • Hirokazu Okayama
    • Mai Ashizawa
    • Masaru Noda
    • Keita Aoto
    • Motonobu Saito
    • Tomoyuki Monma
    • Shinji Ohki
    • Masahiko Shibata
    • Seiichi Takenoshita
    • Koji Kono
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Tract Surgery, Fukushima Medical University, Fukushima 960‑1295, Japan, Department of Breast Surgery, Fukushima Medical University, Fukushima 960‑1295, Japan
  • Pages: 2604-2610
    |
    Published online on: December 8, 2017
       https://doi.org/10.3892/ol.2017.7556
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Abstract

Enhancement of antibody-dependent cellular cytotoxicity (ADCC) with some modalities may be a promising approach to enhance the efficacy of therapeutic monoclonal antibodies (mAbs). It has previously been demonstrated that the removal of fucose from antibody oligosaccharides (defucosylation) leads to augmentation of ADCC activity. To establish clinically relevant evidence of this procedure, the present study evaluated trastuzumab‑ and cetuximab‑mediated ADCC by comparing defucosylated mAbs with conventional mAbs using peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from 20 patients with gastrointestinal tract cancer and 10 healthy volunteers. ADCCs were measured using PBMCs as effector cells and two gastric cancer cell lines as target cells. ADCCs were significantly enhanced with defucosylated mAbs compared with conventional mAbs using PBMC from the healthy donors and patients with cancer. The results confirmed that the cetuximab‑ and trastuzumab‑mediated ADCCs in advanced disease were impaired in comparison to those in early disease or healthy individuals. However, when the defucosylated mAbs were used instead of the conventional mAbs, the ADCC activities in the advanced cases were almost comparable with those in early disease or healthy individuals. Furthermore, the expression of ADCC associated molecules were modified toward immunosuppressive status with a mitogen‑activated protein kinase inhibitor in vitro, the conventional cetuximab‑ and trastuzumab‑mediated ADCC was downregulated, and the defucosylated mAbs overcome the downregulation of ADCC. In conclusion, defucosylated therapeutic mAbs may enhance ADCC activities in patients with cancer, which may lead to more effective anti‑cancer treatments.
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1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Bang YJ, Van Cutsem E, Feyereislova A, Chung HC, Shen L, Sawaki A, Lordick F, Ohtsu A, Omuro Y, Satoh T, et al: Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer (ToGA): A phase 3, open-label, randomised controlled trial. Lancet. 376:687–697. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Li K and Li J: Current molecular targeted therapy in advanced gastric cancer: A comprehensive review of therapeutic mechanism, clinical trials, and practical application. Gastroenterol Res Pract. 2016:41056152016. View Article : Google Scholar : PubMed/NCBI

4 

Arienti C, Zanoni M, Pignatta S, Del Rio A, Carloni S, Tebaldi M, Tedaldi G and Tesei A: Preclinical evidence of multiple mechanisms underlying trastuzumab resistance in gastric cancer. Oncotarget. 7:18424–18439. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Koene HR, Kleijer M, Algra J, Roos D, von dem Borne AE and de Haas M: Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype. Blood. 90:1109–1114. 1997.PubMed/NCBI

6 

Shields RL, Namenuk AK, Hong K, Meng YG, Rae J, Briggs J, Xie D, Lai J, Stadlen A, Li B, et al: High resolution mapping of the binding site on human IgG1 for Fc gamma RI Fc gamma RII Fc gamma RIII, and FcRn and design of IgG1 variants with improved binding to the Fc gamma R. J Biol Chem. 276:6591–6604. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Warmerdam PA, van de Winkel JG, Vlug A, Westerdaal NA and Capel PJ: A single amino acid in the second Ig-like domain of the human Fc gamma receptor II is critical for human IgG2 binding. J Immunol. 147:1338–1343. 1991.PubMed/NCBI

8 

Watanabe M, Kono K, Kawaguchi Y, Mizukami Y, Mimura K, Maruyama T, Izawa S and Fujii H: NK cell dysfunction with down-regulated CD16 and up-regulated CD56 molecules in patients with esophageal squamous cell carcinoma. Dis Esophagus. 23:675–681. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Musolino A, Naldi N, Bortesi B, Pezzuolo D, Capelletti M, Missale G, Laccabue D, Zerbini A, Camisa R, Bisagni G, et al: Immunoglobulin G fragment C receptor polymorphisms and clinical efficacy of trastuzumab-based therapy in patients with HER-2/neu-positive metastatic breast cancer. J Clin Oncol. 26:1789–1796. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Wormald S, Milla L and O'Connor L: Association of candidate single nucleotide polymorphisms with somatic mutation of the epidermal growth factor receptor pathway. BMC Med Genomics. 6:432013. View Article : Google Scholar : PubMed/NCBI

11 

Shields RL, Lai J, Keck R, O'Connell LY, Hong K, Meng YG, Weikert SH and Presta LG: Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity. J Biol Chem. 277:26733–26740. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Shinkawa T, Nakamura K, Yamane N, Shoji-Hosaka E, Kanda Y, Sakukrada M, Uchida K, Anazawa H, Satoh M, Yamasaki M, et al: The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity. J Biol Chem. 278:3466–3473. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Niwa R, Shoji-Hosaka E, Sakurada M, Shinkawa T, Uchida K, Nakamura K, Matsushima K, Ueda R, Hanai N and Shitara K: Defucosylated chimeric anti-CC chemokine receptor 4 IgG1 with enhanced antibody-dependent cellular cytotoxicity shows potent therapeutic activity to T-cell leukemia and lymphoma. Cancer Res. 64:2127–2133. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Okazaki A, Shoji-Hosaka E, Nakamura K, Wakitani M, Uchida K, Kakita S, Tsumoto K, Kumagai I and Shitara K: Fucose depletion from human IgG1 oligosaccharide enhances binding enthalpy and association rate between IgG1 and FcgammaRIIIa. J Mol Biol. 336:1239–1249. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Niwa R, Hatanaka S, Shoji-Hosaka E, Sakurada M, Kobayashi Y, Uehara A, Yokoi H, Nakamura K and Shitara K: Enhancement of the antibody-dependent cellular cytotoxicity of low-fucose IgG1 Is independent of FcgammaRIIIa functional polymorphism. Clin Cancer Res. 10:6248–6255. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Suzuki E, Niwa R, Saji S, Muta M, Hirose M, Iida S, Shiotsu Y, Satoh M, Shitara K, Kondo M and Toi M: A nonfucosylated anti-HER2 antibody augments antibody-dependent cellular cytotoxicity in breast cancer patients. Clin Cancer Res. 13:1875–1882. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR and Freshney RI: Check your cultures! A list of cross-contaminated or misidentified cell lines. Int J Cancer. 127:1–8. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Shiraishi K, Mimura K, Izawa S, Inoue A, Shiba S, Maruyama T, Watanabe M, Kawaguchi Y, Inoue M, Fujii H and Kono K: Lapatinib acts on gastric cancer through both antiproliferative function and augmentation of trastuzumab-mediated antibody-dependent cellular cytotoxicity. Gastric Cancer. 16:571–580. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Carter P, Presta L, Gorman CM, Ridgway JB, Henner D, Wong WL, Rowland AM, Kotts C, Carverr ME and Shepard HM: Humanization of an anti-p185HER2 antibody for human cancer therapy. Proc Natl Acad Sci USA. 89:pp. 4285–4289. 1992; View Article : Google Scholar : PubMed/NCBI

20 

DrugBANK. https://www.drugbank.ca/drugs/DB00002

21 

Yamane-Ohnuki N, KInoshita S, Inoue-Urakubo M, Kusunoki M, Iida S, Nakano R, Wakitani M, Niwa R, Sakurada M, Uchida K, et al: Establishment of FUT8 knockout Chinese hamster ovary cells: An ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity. Biotechnol Bioeng. 87:614–622. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Mimura K, Kamiya T, Shiraishi K, Kua LF, Shabbir A, So J, Yong WP, Suzuki Y, Yoshimoto Y, Nakano T, et al: Therapeutic potential of highly cytotoxic natural killer cells for gastric cancer. Int J Cancer. 135:1390–1398. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Kono K, Takahashi A, Ichihara F, Sugai H, Fujii H and Matsumoto Y: Impaired antibody-dependent cellular cytotoxicity mediated by herceptin in patients with gastric cancer. Cancer Res. 62:5813–5817. 2002.PubMed/NCBI

24 

Mimura K, Kono K, Hanawa M, Kanzaki M, Nakao A, Ooi A and Fujii H: Trastuzumab-mediated antibody-dependent cellular cytotoxicity against esophageal squamous cell carcinoma. Clin Cancer Res. 11:4898–4904. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Moretta L, Locatelli F, Pende D, Marcenaro E, Mingari MC and Moretta A: Killer Ig-like receptor-mediated control of natural killer cell alloreactivity in haploidentical hematopoietic stem cell transplantation. Blood. 117:764–771. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Bae DS, Hwang YK and Lee JK: Importance of NKG2D-NKG2D ligands interaction for cytolytic activity of natural killer cell. Cell Immunol. 276:122–127. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Okita R, Mougiakakos D, Ando T, Mao Y, Sarhan D, Wennerberg E, Seliger B, Lundqvist A, Mimura K and Kiessling R: HER2/HER3 signaling regulates NK cell-mediated cytotoxicity via MHC class I chain-related molecule A and B expression in human breast cancer cell lines. J Immunol. 188:2136–2145. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Watanabe Y, Asano R, Arai K, Shimomura I, Ogata H, Kawaguchi H, Hayashi H, Ohtsuka H, Yoshida H, Katayose Y, et al: In vitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody. Oncol Rep. 26:949–955. 2011.PubMed/NCBI

29 

Baselga J, Carbonell X, Castañeda-Soto NJ, Clemens M, Green M, Harvey V, Morales S, Barton C and Ghahramani P: Phase II study of efficacy, safety, and pharmacokinetics of trastuzumab monotherapy administered on a 3-weekly schedule. J Clin Oncol. 23:2162–2171. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Hudis CA: Trastuzumab-mechanism of action and use in clinical practice. N Engl J Med. 357:39–51. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Slamon DJ, Leyland-Jones B, Shak S, Fuchs H, Paton V, Bajamonde A, Fleming T, Eiermann W, Wolter J, Pegram M, et al: Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2. N Engl J Med. 344:783–792. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Kawaguchi Y, Kono K, Mimura K, Mitsui F, Sugai H, Akaike H and Fujii H: Targeting EGFR and HER-2 with cetuximab- and trastuzumab-mediated immunotherapy in oesophageal squamous cell carcinoma. Br J Cancer. 97:494–501. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Kawaguchi Y, Kono K, Mimura K, Sugai H, Akaike H and Fujii H: Cetuximab induce antibody-dependent cellular cytotoxicity against EGFR-expressing esophageal squamous cell carcinoma. Int J Cancer. 120:781–787. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Omori S, Hida M, Fujita H, Takahashi H, Tanimura S, Kohno M and Awazu M: Extracellular signal-regulated kinase inhibition slows disease progression in mice with polycystic kidney disease. J Am Soc Nephrol. 17:1604–1614. 2006. View Article : Google Scholar : PubMed/NCBI

35 

Fujiwara Y, Hosokawa Y, Watanabe K, Tanimura S, Ozaki K and Kohno M: Blockade of the phosphatidylinositol-3-kinase-Akt signaling pathway enhances the induction of apoptosis by microtubule-destabilizing agents in tumor cells in which the pathway is constitutively activated. Mol Cancer Ther. 6:1133–1142. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Nakajima T, Okayama H, Ashizawa M, Noda M, Aoto K, Saito M, Monma T, Ohki S, Shibata M, Takenoshita S, Takenoshita S, et al: Augmentation of antibody-dependent cellular cytotoxicity with defucosylated monoclonal antibodies in patients with GI-tract cancer. Oncol Lett 15: 2604-2610, 2018.
APA
Nakajima, T., Okayama, H., Ashizawa, M., Noda, M., Aoto, K., Saito, M. ... Kono, K. (2018). Augmentation of antibody-dependent cellular cytotoxicity with defucosylated monoclonal antibodies in patients with GI-tract cancer. Oncology Letters, 15, 2604-2610. https://doi.org/10.3892/ol.2017.7556
MLA
Nakajima, T., Okayama, H., Ashizawa, M., Noda, M., Aoto, K., Saito, M., Monma, T., Ohki, S., Shibata, M., Takenoshita, S., Kono, K."Augmentation of antibody-dependent cellular cytotoxicity with defucosylated monoclonal antibodies in patients with GI-tract cancer". Oncology Letters 15.2 (2018): 2604-2610.
Chicago
Nakajima, T., Okayama, H., Ashizawa, M., Noda, M., Aoto, K., Saito, M., Monma, T., Ohki, S., Shibata, M., Takenoshita, S., Kono, K."Augmentation of antibody-dependent cellular cytotoxicity with defucosylated monoclonal antibodies in patients with GI-tract cancer". Oncology Letters 15, no. 2 (2018): 2604-2610. https://doi.org/10.3892/ol.2017.7556
Copy and paste a formatted citation
x
Spandidos Publications style
Nakajima T, Okayama H, Ashizawa M, Noda M, Aoto K, Saito M, Monma T, Ohki S, Shibata M, Takenoshita S, Takenoshita S, et al: Augmentation of antibody-dependent cellular cytotoxicity with defucosylated monoclonal antibodies in patients with GI-tract cancer. Oncol Lett 15: 2604-2610, 2018.
APA
Nakajima, T., Okayama, H., Ashizawa, M., Noda, M., Aoto, K., Saito, M. ... Kono, K. (2018). Augmentation of antibody-dependent cellular cytotoxicity with defucosylated monoclonal antibodies in patients with GI-tract cancer. Oncology Letters, 15, 2604-2610. https://doi.org/10.3892/ol.2017.7556
MLA
Nakajima, T., Okayama, H., Ashizawa, M., Noda, M., Aoto, K., Saito, M., Monma, T., Ohki, S., Shibata, M., Takenoshita, S., Kono, K."Augmentation of antibody-dependent cellular cytotoxicity with defucosylated monoclonal antibodies in patients with GI-tract cancer". Oncology Letters 15.2 (2018): 2604-2610.
Chicago
Nakajima, T., Okayama, H., Ashizawa, M., Noda, M., Aoto, K., Saito, M., Monma, T., Ohki, S., Shibata, M., Takenoshita, S., Kono, K."Augmentation of antibody-dependent cellular cytotoxicity with defucosylated monoclonal antibodies in patients with GI-tract cancer". Oncology Letters 15, no. 2 (2018): 2604-2610. https://doi.org/10.3892/ol.2017.7556
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