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Article

Dihydroartemisinin treatment of multiple myeloma cells causes activation of c-Jun leading to cell apoptosis

  • Authors:
    • Yong Wang
    • Xiaoyuan Xu
    • Xiaojian Wu
    • Weibin Chen
    • Fangmei Huang
    • Xiaomin Gui
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The Affiliated Hospital of Jiujiang University College of Medicine, Jiujiang, Jiangxi 332000, P.R. China, Key Laboratory of System Bio‑Medicine of Jiangxi, Jiujiang University, Jiujiang, Jiangxi 332000, P.R. China
  • Pages: 2562-2566
    |
    Published online on: December 11, 2017
       https://doi.org/10.3892/ol.2017.7582
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Abstract

The aim of the present study was to investigate the effect of dihydroartemisinin (DHA) on a multiple myeloma cell line. An MTT assay, flow cytometry and reverse transcription‑polymerase chain reaction (RT‑PCR) were used for the analysis of cell viability, cell cycle distribution and c‑Jun N‑terminal kinase (JNK) expression, respectively. Treatment of U266 cells using DHA caused a significant (P<0.05) decrease in cell viability compared with the control cells. An increase in the concentration of DHA from 1 to 100 µmol/l reduced cell viability from 87 to 35% compared with 100% in the control cultures at 48 h. A significant (P<0.05) increase was observed in the sub‑G0/G1 phase population of the U266 cells with an increase in DHA concentration from 1 to 100 µmol/l. Treatment with 1, 3, 10, 30 and 100 µmol/l concentrations of DHA increased the sub‑G0/G1 phase cell population to 3.13, 8.25, 24.91, 31.47 and 38.54%, respectively. RT‑PCR analysis of DHA‑treated or ‑untreated U266 cells after 48 h demonstrated a significant (P<0.01) increase in caspase‑3 expression. Treatment of the cells for 48 h with DHA led to a significant increase in c‑Jun expression. DHA treatment at 1, 3, 10, 30 and 100 µmol/l concentrations caused an increase in the level of c‑Jun by 0.174±0.001, 0.254±0.002, 0.387±0.001, 0.502±0.003 and 0.679±0.005, respectively, compared with 0.982±0.001 in the control cells. The addition of SP600125 to the cells incubated with DHA resulted in a significant decrease in the caspase‑3 and c‑Jun expression levels compared with those cells incubated with DHA alone. These findings confirm that treatment with DHA increased caspase‑3 and c‑Jun expression in the U266 cells through activation of the JNK signaling pathway. Thus, DHA inhibited proliferation of multiple myeloma cells by interfering with the JNK signaling pathway.
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View References

1 

Wei ZL and Wang XH: The development of NF-κB in the multiple myeloma. Med Res. 36:98–101. 2007.

2 

Spisek R, Charalambous A, Mazumder A, Vesole DH, Jagannath S and Dhodapkar MV: Bortezomib enhances dendritic cell (DC)-mediated induction of immunity to human myeloma via exposure of cell surface heat shock protein 90 on dying tumor cells: Therapeutic implications. Blood. 109:4839–4845. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Carocho M and Ferreira IC: A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem Toxicol. 51:15–25. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Mecocci P and Polidori MC: Antioxidant clinical trials in mild cognitive impairment and Alzheimer's disease. Biochim Biophys Acta. 1822:631–638. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Hawkes CA, Ng V and McLaurin JA: Small molecule inhibitors of Aβ-aggregation and neurotoxicity. Drug Dev Res. 70:111–124. 2009. View Article : Google Scholar

6 

Joynera PM and Cichewicz RH: Bringing natural products into the fold-exploring the therapeutic lead potential of secondary metabolites for the treatment of protein-misfolding related neurodegenerative diseases. Nat Prod Rep. 28:26–47. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Meshnick SR: Artemisinin: Mechanisms of action, resistance and toxicity. Int J Parasitol. 32:1655–1660. 2002. View Article : Google Scholar : PubMed/NCBI

8 

O'Neill PM: Medicinal chemistry: A worthy adversary for malaria. Nature. 430:838–839. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Efferth T, Dunstan H, Sauerbrey A, Miyachi H and Chitambar CR: The anti-malarial artesunate is also active against cancer. Int J Oncol. 18:767–773. 2001.PubMed/NCBI

10 

Huang XJ, Ma ZQ, Zhang WP, Lu YB and Wei EQ: Dihydroartemisinin exerts cytotoxic effects and inhibits hypoxia inducible factor-1alpha activation in C6 glioma cells. J Pharm Pharmacol. 59:849–856. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Nam W, Tak J, Ryu JK, Jung M, Yook JI, Kim HJ and Cha IH: Effects of artemisinin and its derivatives on growth inhibition and apoptosis of oral cancer cells. Head Neck. 29:335–340. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Singh NP and Lai H: Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sci. 70:49–56. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Chen T, Li M, Zhang R and Wang H: Dihydroartemisinin induces apoptosis and sensitizes human ovarian cancer cells to carboplatin therapy. J Cell Mol Med. 13:1358–1370. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Liang XM and Yang KD: Caspase, JNK/SAPK, P38 MAPK and apoptosis. Foreign Med Sci (Section Hygiene). 35:5–10. 2008.(In Chinese).

15 

Du L, Wang FY, Zhang L and Liu T: Advance in the research of JNK dependent apoptosis. China Trop Med. 18:841–844. 2008.(In Chinese).

16 

Xiao Y, Yang FQ, Li SP, Gao JL, Hu G, Lao SC, Conceição EL, Fung KP, Wangl YT and Lee SM: Furanodiene induces G2/M cell cycle arrest and apoptosis through MAPK signaling and mitochondria-caspase pathway in human hepatocellular carcinoma cells. Cancer Biol Ther. 6:1044–1050. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Papa S, Zazzeroni F, Pham CG, Bubici C and Franzoso G: Linking JNK signaling to NF-kappaB: A key to survival. J Cell Sci. 117:5197–5208. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Murakami Y, Aizu-Yokota E, Sonoda Y, Ohta S and Kasahara T: Suppression of endoplasmic reticulum stress induced caspase activation and cell death by the over expression of Bcl-xl or Bcl-2. J Biochem. 141:401–410. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Peng J, Mao XO, Stevenson FF, Hsu M and Andersen JK: The herbicide paraquat induces dopaminergic nigral apoptosis through sustained activation of the JNK pathway. J Biol Chem. 279:32626–32632. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Largo C, Alvarez S, Saez B, Blesa D, Martin-Subero JI, González-García I, Brieva JA, Dopazo J, Siebert R, Calasanz MJ and Cigudosa JC: Identification of overexpressed genes in frequently gained/amplified chromosome regions in multiple myeloma. Hematologica. 91:184–191. 2004.

21 

Lin HH, Chen JH, Kuo WH and Wang CJ: Chemopreventive properties of Hibiscus sabdariffa L. on human gastric carcinoma cells through apoptosis induction and JNK/p38 MAPK signaling activation. Chem Biol Interac. 165:59–75. 2007. View Article : Google Scholar

22 

Junttila MR, Li SP and Westermarck J: Phosphatase-mediated crosstalk between MAPK signaling pathways in the regulation of cell survival. FASEB J. 22:954–965. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Xu X, Wu X, Chen W, Huang F and Gui X: Dihydroartemisinin treatment of multiple myeloma cells causes activation of c-Jun leading to cell apoptosis. Oncol Lett 15: 2562-2566, 2018.
APA
Wang, Y., Xu, X., Wu, X., Chen, W., Huang, F., & Gui, X. (2018). Dihydroartemisinin treatment of multiple myeloma cells causes activation of c-Jun leading to cell apoptosis. Oncology Letters, 15, 2562-2566. https://doi.org/10.3892/ol.2017.7582
MLA
Wang, Y., Xu, X., Wu, X., Chen, W., Huang, F., Gui, X."Dihydroartemisinin treatment of multiple myeloma cells causes activation of c-Jun leading to cell apoptosis". Oncology Letters 15.2 (2018): 2562-2566.
Chicago
Wang, Y., Xu, X., Wu, X., Chen, W., Huang, F., Gui, X."Dihydroartemisinin treatment of multiple myeloma cells causes activation of c-Jun leading to cell apoptosis". Oncology Letters 15, no. 2 (2018): 2562-2566. https://doi.org/10.3892/ol.2017.7582
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Xu X, Wu X, Chen W, Huang F and Gui X: Dihydroartemisinin treatment of multiple myeloma cells causes activation of c-Jun leading to cell apoptosis. Oncol Lett 15: 2562-2566, 2018.
APA
Wang, Y., Xu, X., Wu, X., Chen, W., Huang, F., & Gui, X. (2018). Dihydroartemisinin treatment of multiple myeloma cells causes activation of c-Jun leading to cell apoptosis. Oncology Letters, 15, 2562-2566. https://doi.org/10.3892/ol.2017.7582
MLA
Wang, Y., Xu, X., Wu, X., Chen, W., Huang, F., Gui, X."Dihydroartemisinin treatment of multiple myeloma cells causes activation of c-Jun leading to cell apoptosis". Oncology Letters 15.2 (2018): 2562-2566.
Chicago
Wang, Y., Xu, X., Wu, X., Chen, W., Huang, F., Gui, X."Dihydroartemisinin treatment of multiple myeloma cells causes activation of c-Jun leading to cell apoptosis". Oncology Letters 15, no. 2 (2018): 2562-2566. https://doi.org/10.3892/ol.2017.7582
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