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Article

Development and in vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer

  • Authors:
    • Bao‑Zhong Jin
    • Xiao‑Qi Dong
    • Xin Xu
    • Feng‑He Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oral Surgery, Stomatological Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Oral Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China
  • Pages: 2541-2549
    |
    Published online on: December 13, 2017
       https://doi.org/10.3892/ol.2017.7613
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Abstract

The present study focused on the development of a mucoadhesive patch of methotrexate (MTX) for targeted delivery in oral cancer. Initially, MTX‑loaded liposomes were prepared using the thin film hydration method, and had a mean diameter of 105.7‑137.4 nm and percentage entrapment efficiency of 54.6±3.5. These liposomes were cast in optimized mucoadhesive film. The film was characterized by its release pattern, thickness, weight and percentage swelling index and the sustained release profile of the optimized film was evaluated. The developed liposomes and liposomes cast in the film formulation were evaluated for cytotoxicity in HSC‑3 cells using an MTT assay, and a significant decrease in the half maximal inhibitory concentration of MTX was identified with the MTX‑entrapped liposomal film, M‑LP‑F7. The results of the mitochondria-dependent intrinsic pathway demonstrated that there was significant mitochondrial membrane potential disruption with M‑LP‑F7 compared with the plain drug. M‑LP‑F7 increased the rate of apoptosis in HSC‑3 cells by almost 3‑fold. Elevated levels of reactive oxygen species provided evidence that M‑LP‑F7 exerts a pro‑oxidant effect in HSC-3 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Jin BZ, Dong XQ, Xu X and Zhang FH: Development and in vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer. Oncol Lett 15: 2541-2549, 2018.
APA
Jin, B., Dong, X., Xu, X., & Zhang, F. (2018). Development and in vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer. Oncology Letters, 15, 2541-2549. https://doi.org/10.3892/ol.2017.7613
MLA
Jin, B., Dong, X., Xu, X., Zhang, F."Development and in vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer". Oncology Letters 15.2 (2018): 2541-2549.
Chicago
Jin, B., Dong, X., Xu, X., Zhang, F."Development and in vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer". Oncology Letters 15, no. 2 (2018): 2541-2549. https://doi.org/10.3892/ol.2017.7613
Copy and paste a formatted citation
x
Spandidos Publications style
Jin BZ, Dong XQ, Xu X and Zhang FH: Development and in vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer. Oncol Lett 15: 2541-2549, 2018.
APA
Jin, B., Dong, X., Xu, X., & Zhang, F. (2018). Development and in vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer. Oncology Letters, 15, 2541-2549. https://doi.org/10.3892/ol.2017.7613
MLA
Jin, B., Dong, X., Xu, X., Zhang, F."Development and in vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer". Oncology Letters 15.2 (2018): 2541-2549.
Chicago
Jin, B., Dong, X., Xu, X., Zhang, F."Development and in vitro evaluation of mucoadhesive patches of methotrexate for targeted delivery in oral cancer". Oncology Letters 15, no. 2 (2018): 2541-2549. https://doi.org/10.3892/ol.2017.7613
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