Actein inhibits cell proliferation and migration and promotes cell apoptosis in human non-small cell lung cancer cells

  • Authors:
    • Yuanyuan Zhang
    • Jianchun Lian
    • Xiaowei Wang
  • View Affiliations

  • Published online on: December 20, 2017     https://doi.org/10.3892/ol.2017.7668
  • Pages: 3155-3160
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Abstract

Non-small cell lung cancer (NSCLC) is the leading cause of death in smokers and the most common cause for cancer mortality in both males and females in the United States. Predisposition of this malignancy to distant metastasis leads to poor prognosis; therefore, it is urgent to discover novel therapeutic agents for metastatic NSCLC. The present study aimed to investigate the effects of actein treatment on NSCLC cell growth and migration. Cell viability assays demonstrated that administration of actein markedly inhibited NSCLC cell proliferation in a dose- and time‑dependent manner. Transwell assays demonstrated that actein treatment suppressed cell migration and invasion in two NSCLC cell lines, A549 and 95D. Furthermore, treatment with actein remarkably increased the activities of caspase‑3 and ‑9 in NSCLC cells. The protein expression levels of cytoplasmic BCL2 apoptosis regulator (Bcl‑2) and BCL2 associated X (Bax) were markedly decreased, while the protein expression levels of mitochondrial Bax, caspase‑3, ‑9 and cytochrome c were upregulated following actein treatment, as evidenced by western blot analysis. The present results demonstrated that actein inhibited cell proliferation and metastasis and promoted cell apoptosis in NSCLC cells, which indicated that actein administration might serve as a potential therapeutic strategy for the treatment of NSCLC in the clinic.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang Y, Lian J and Wang X: Actein inhibits cell proliferation and migration and promotes cell apoptosis in human non-small cell lung cancer cells. Oncol Lett 15: 3155-3160, 2018
APA
Zhang, Y., Lian, J., & Wang, X. (2018). Actein inhibits cell proliferation and migration and promotes cell apoptosis in human non-small cell lung cancer cells. Oncology Letters, 15, 3155-3160. https://doi.org/10.3892/ol.2017.7668
MLA
Zhang, Y., Lian, J., Wang, X."Actein inhibits cell proliferation and migration and promotes cell apoptosis in human non-small cell lung cancer cells". Oncology Letters 15.3 (2018): 3155-3160.
Chicago
Zhang, Y., Lian, J., Wang, X."Actein inhibits cell proliferation and migration and promotes cell apoptosis in human non-small cell lung cancer cells". Oncology Letters 15, no. 3 (2018): 3155-3160. https://doi.org/10.3892/ol.2017.7668