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Article

Effect of silencing colon cancer-associated transcript 2 on the proliferation, apoptosis and autophagy of gastric cancer BGC-823 cells

  • Authors:
    • Zhao‑Yan Yu
    • Zi Wang
    • Ke‑Yue Lee
    • Ping Yuan
    • Jie Ding
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China, Department of Oncology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China, Department of Hepatobiliary Surgery, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China
  • Pages: 3127-3132
    |
    Published online on: December 21, 2017
       https://doi.org/10.3892/ol.2017.7677
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Abstract

The role of long non-coding RNAs (lncRNAs) in the carcinogenesis and progression of tumors has been receiving increasing attention. Colon cancer‑associated transcript 2 (CCAT2), a type of oncogenic lncRNA, is regarded as a novel biomarker of poor prognosis and metastasis in various types of cancer. However, the molecular contributions of CCAT2 to gastric cancer (GC) progression remain largely unclear. The aim of the present study was to demonstrate the effect of silencing CCAT2 on the biological behavior of GC BGC‑823 cells and illustrate the potential underlying molecular mechanisms. A short hairpin RNA interference plasmid pRNAT‑U6.1‑CCAT2 targeting CCAT2 was successfully constructed. At 48 h after transfection with the interference plasmid, the survival rate of BGC‑823 cells was significantly decreased, as determined by the MTT assay. In addition, RT‑qPCR results revealed that CCAT2 gene expression was effectively suppressed by the transfection, while POU domain class 5 transcription factor 1B (POU5F1B) gene expression was significantly decreased. Terminal deoxynucleotidyl transferase dUTP nick end labeling assay further revealed that the apoptotic index was significantly higher in the interference group. Western blot analysis also demonstrated that the expression of beclin‑1 protein was significantly increased, whereas the expression levels of phosphoinositide 3‑kinase (PI3K) and mammalian target of rapamycin (mTOR) proteins were downregulated in the interference group. In conclusion, CCAT2 was able to positively regulate the expression of POU5F1B gene. Furthermore, silencing of CCAT2 gene inhibited the proliferation of BGC‑823 cells, as well as induced apoptosis and autophagy in BGC‑823 cells, by suppression of the PI3K/mTOR signaling pathways.
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1 

Brenner H, Rothenbacher D and Arndt V: Epidemiology of stomach cancer. Methods Mol Biol. 472:467–477. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Zhang M and Du X: Noncoding RNAs in gastric cancer: Research progress and prospects. World J Gastroenterol. 22:6610–6618. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Zhang R, Xia LQ, Lu WW, Zhang J and Zhu JS: LncRNAs and cancer. Oncol Lett. 12:1233–1239. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Ling H, Spizzo R, Atlasi Y, Nicoloso M, Shimizu M, Redis RS, Nishida N, Gafà R, Song J, Guo Z, et al: CCAT2, a novel noncoding RNA mapping to 8q24, underlies metastatic progression and chromosomal instability in colon cancer. Genome Res. 23:1446–1461. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Fan YH, Fang H, Ji CX, Xie H, Xiao B and Zhu XG: Long noncoding RNA CCAT2 can predict metastasis and poor prognosis: A meta-analysis. Clin Chim Acta. 466:120–126. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Wang CY, Hua L, Yao KH, Chen JT, Zhang JJ and Hu JH: Long non-coding RNA CCAT2 is up-regulated in gastric cancer and associated with poor prognosis. Int J Clin Exp Pathol. 8:779–785. 2015.PubMed/NCBI

7 

Wang YJ, Liu JZ, Lv P, Dang Y, Gao JY and Wang Y: Long non-coding RNA CCAT2 promotes gastric cancer proliferation and invasion by regulating the E-cadherin and LATS2. Am J Cancer Res. 6:2651–2660. 2016.PubMed/NCBI

8 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Zhao X, Ma C, Cai X, Lei D, Liu D, Xu F, Jin T, Liu J and Pan X: RNA interference of caveolin-1 via lentiviral vector inhibits growth of hypopharyngeal squamous cell carcinoma FaDu cells in vitro and in vivo. Asian Pac J Cancer Prev. 12:397–401. 2011.PubMed/NCBI

10 

Sun W, Yang Y, Xu C, Xie Y and Guo J: Roles of long noncoding RNAs in gastric cancer and their clinical applications. J Cancer Res ClinOncol. 142:2231–2237. 2016. View Article : Google Scholar

11 

Chen S, Wu H, Lv N, Wang H, Wang Y, Tang Q, Shao H and Sun C: LncRNA CCAT2 predicts poor prognosis and regulates growth and metastasis in small cell lung cancer. Biomed Pharmacother. 82:583–588. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Ouyang S, Zhang P, Wang J, Huang Z and Liao L: Expression of long non-coding RNA colon cancer associated transcript 2 and its clinicopathologic significance in oral squamous cell carcinoma. Zhonghua Kou Qiang Yi XueZaZhi. 51:286–291. 2016.(In Chinese).

13 

Huang S, Qing C, Huang Z and Zhu Y: The long non-coding RNA CCAT2 is up-regulated in ovarian cancer and associated with poor prognosis. Diagn Pathol. 11:492016. View Article : Google Scholar : PubMed/NCBI

14 

Zhou N, Si Z, Li T, Chen G, Zhang Z and Qi H: Long non-coding RNA CCAT2 functions as an oncogene in hepatocellular carcinoma, regulating cellular proliferation, migration and apoptosis. Oncol Lett. 12:132–138. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Redis RS, Vela LE, Lu W, Ferreira de Oliveira J, Ivan C, Rodriguez-Aguayo C, Adamoski D, Pasculli B, Taguchi A, Chen Y, et al: Allele-specific reprogramming of cancer metabolism by the long non-coding RNA CCAT2. Mol Cell. 61:520–534. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Redis RS and Calin GA: The interplay between lnRNAs, SNPs, and protein complexes-what does it mean for cancer metabolism? Mol Cell Oncol. 3:e11663082016. View Article : Google Scholar : PubMed/NCBI

17 

Barry KH, Moore LE, Sampson J, Yan L, Meyer A, Oler AJ, Chung CC, Wang Z, Yeager M, Amundadottir L and Berndt SI: DNA methylation levels at chromosome 8q24 in peripheral blood are associated with 8q24 cancer susceptibility loci. Cancer Prev Res (Phila). 7:1282–1292. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Brisbin AG, Asmann YW, Song H, Tsai YY, Aakre JA, Yang P, Jenkins RB, Pharoah P, Schumacher F, Conti DV, et al: Meta-analysis of 8q24 for seven cancers reveals a locus between NOV and ENPP2 associated with cancer development. BMC Med Genet. 12:1562011. View Article : Google Scholar : PubMed/NCBI

19 

Breyer JP, Dorset DC, Clark TA, Bradley KM, Wahlfors TA, McReynolds KM, Maynard WH, Chang SS, Cookson MS, Smith JA, et al: An expressed retrogene of the master embryonic stem cell gene POU5F1 is associated with prostate cancer susceptibility. Am J Hum Genet. 94:395–404. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Hayashi H, Arao T, Togashi Y, Kato H, Fujita Y, De Velasco MA, Kimura H, Matsumoto K, Tanaka K, Okamoto I, et al: The OCT4 pseudogene POU5F1B is amplified and promotes an aggressive phenotype in gastric cancer. Oncogene. 34:199–208. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Liu G, Pei F, Yang F, Li L, Amin AD, Liu S, Buchan JR and Cho WC: Role of autophagy and apoptosis in non-small-cell lung cancer. Int J Mol Sci. 18:E3672017. View Article : Google Scholar : PubMed/NCBI

22 

Zhou H, Yuan M, Yu Q, Zhou X, Min W and Gao D: Autophagy regulation and its role in gastric cancer and colorectal cancer. Cancer Biomark. 17:1–10. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Fu LL, Cheng Y and Liu B: Beclin-1: Autophagic regulator and therapeutic target in cancer. Int J Biochem Cell Biol. 45:921–924. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Yu ZY, Ding J, Yang XF and Zhang ZM: Significance of autophagy-related protein beclin-1 expression in gastric cancer: A Meta-analysis. Chin J Gen Surg. 24:1389–1395. 2015.

25 

He Y, Zhao X, Subahan NR, Fan L, Gao J and Chen H: The prognostic value of autophagy-related markers beclin-1 and microtubule-associated protein light chain 3B in cancers: A systematic review and meta-analysis. Tumour Biol. 35:7317–7326. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Cao QH, Liu F, Yang ZL, Fu XH, Yang ZH, Liu Q, Wang L, Wan XB and Fan XJ: Prognostic value of autophagy related proteins ULK1, Beclin 1, ATG3, ATG5, ATG7, ATG9, ATG10, ATG12, LC3B and p62/SQSTM1 in gastric cancer. Am J Transl Res. 8:3831–3847. 2016.PubMed/NCBI

27 

Polivka J Jr and Janku F: Molecular targets for cancer therapy in the PI3K/AKT/mTOR pathway. Pharmacol Ther. 142:164–175. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Liu M, Li CM, Chen ZF, Ji R, Guo QH, Li Q, Zhang HL and Zhou YN: Celecoxib regulates apoptosis and autophagy via the PI3K/Akt signaling pathway in SGC-7901 gastric cancer cells. Int J Mol Med. 33:1451–1458. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yu ZY, Wang Z, Lee KY, Yuan P and Ding J: Effect of silencing colon cancer-associated transcript 2 on the proliferation, apoptosis and autophagy of gastric cancer BGC-823 cells. Oncol Lett 15: 3127-3132, 2018.
APA
Yu, Z., Wang, Z., Lee, K., Yuan, P., & Ding, J. (2018). Effect of silencing colon cancer-associated transcript 2 on the proliferation, apoptosis and autophagy of gastric cancer BGC-823 cells. Oncology Letters, 15, 3127-3132. https://doi.org/10.3892/ol.2017.7677
MLA
Yu, Z., Wang, Z., Lee, K., Yuan, P., Ding, J."Effect of silencing colon cancer-associated transcript 2 on the proliferation, apoptosis and autophagy of gastric cancer BGC-823 cells". Oncology Letters 15.3 (2018): 3127-3132.
Chicago
Yu, Z., Wang, Z., Lee, K., Yuan, P., Ding, J."Effect of silencing colon cancer-associated transcript 2 on the proliferation, apoptosis and autophagy of gastric cancer BGC-823 cells". Oncology Letters 15, no. 3 (2018): 3127-3132. https://doi.org/10.3892/ol.2017.7677
Copy and paste a formatted citation
x
Spandidos Publications style
Yu ZY, Wang Z, Lee KY, Yuan P and Ding J: Effect of silencing colon cancer-associated transcript 2 on the proliferation, apoptosis and autophagy of gastric cancer BGC-823 cells. Oncol Lett 15: 3127-3132, 2018.
APA
Yu, Z., Wang, Z., Lee, K., Yuan, P., & Ding, J. (2018). Effect of silencing colon cancer-associated transcript 2 on the proliferation, apoptosis and autophagy of gastric cancer BGC-823 cells. Oncology Letters, 15, 3127-3132. https://doi.org/10.3892/ol.2017.7677
MLA
Yu, Z., Wang, Z., Lee, K., Yuan, P., Ding, J."Effect of silencing colon cancer-associated transcript 2 on the proliferation, apoptosis and autophagy of gastric cancer BGC-823 cells". Oncology Letters 15.3 (2018): 3127-3132.
Chicago
Yu, Z., Wang, Z., Lee, K., Yuan, P., Ding, J."Effect of silencing colon cancer-associated transcript 2 on the proliferation, apoptosis and autophagy of gastric cancer BGC-823 cells". Oncology Letters 15, no. 3 (2018): 3127-3132. https://doi.org/10.3892/ol.2017.7677
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