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Article

Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells

  • Authors:
    • Yi‑Chen Wu
    • Xiang Liu
    • Jiu‑Li Wang
    • Xiang‑Liu Chen
    • Lan Lei
    • Jing Han
    • You‑Shui Jiang
    • Zhi‑Qiang Ling
  • View Affiliations / Copyright

    Affiliations: Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, Hangzhou, Zhejiang 310022, P.R. China, Zhejiang Agricultural Group Co., Ltd., Hangzhou, Zhejiang 310021, P.R. China
  • Pages: 3109-3120
    |
    Published online on: December 27, 2017
       https://doi.org/10.3892/ol.2017.7692
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Abstract

Cancer prevention using natural micronutrition on epigenetic mechanisms primarily revolves around plant extracts. However, the role of macronutrition, including animal peptides, on epigenetic modification in cancer has been elusive. In traditional Chinese medicine, the soft‑shelled turtle has a long‑history of being a functional food that strengthens immunity through unknown mechanisms. The present study aimed to investigate the impact of soft‑shelled turtle peptide on microRNA (miRNA) expression in gastric cancer (GC) cells and to analyze the potential anticancer mechanisms for GC. Affymetrix GeneChip miRNA 3.0 Array and quantitative polymerase chain reaction were used to detect the miRNA expression profile in human GC AGS cells treated with the soft‑shelled turtle peptide. The results demonstrated that 101 miRNAs (49 upregulated miRNAs and 52 downregulated miRNAs) were significantly differentially expressed in the AGS cells following soft‑shelled turtle peptide treatment. Several tumor suppressor miRNAs were upregulated markedly, including miRNA‑375, let‑7d, miRNA‑429, miRNA‑148a/148b and miRNA‑34a. Pathway analysis indicated that soft‑shelled turtle peptide may function with anticancer properties through the Hippo signaling pathway and the forkhead box O signaling pathway. Therefore, these results demonstrated that soft‑shelled turtle peptide has the capacity to influence cancer‑related pathways through the regulation of miRNA expression in GC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wu YC, Liu X, Wang JL, Chen XL, Lei L, Han J, Jiang YS and Ling ZQ: Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells. Oncol Lett 15: 3109-3120, 2018.
APA
Wu, Y., Liu, X., Wang, J., Chen, X., Lei, L., Han, J. ... Ling, Z. (2018). Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells. Oncology Letters, 15, 3109-3120. https://doi.org/10.3892/ol.2017.7692
MLA
Wu, Y., Liu, X., Wang, J., Chen, X., Lei, L., Han, J., Jiang, Y., Ling, Z."Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells". Oncology Letters 15.3 (2018): 3109-3120.
Chicago
Wu, Y., Liu, X., Wang, J., Chen, X., Lei, L., Han, J., Jiang, Y., Ling, Z."Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells". Oncology Letters 15, no. 3 (2018): 3109-3120. https://doi.org/10.3892/ol.2017.7692
Copy and paste a formatted citation
x
Spandidos Publications style
Wu YC, Liu X, Wang JL, Chen XL, Lei L, Han J, Jiang YS and Ling ZQ: Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells. Oncol Lett 15: 3109-3120, 2018.
APA
Wu, Y., Liu, X., Wang, J., Chen, X., Lei, L., Han, J. ... Ling, Z. (2018). Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells. Oncology Letters, 15, 3109-3120. https://doi.org/10.3892/ol.2017.7692
MLA
Wu, Y., Liu, X., Wang, J., Chen, X., Lei, L., Han, J., Jiang, Y., Ling, Z."Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells". Oncology Letters 15.3 (2018): 3109-3120.
Chicago
Wu, Y., Liu, X., Wang, J., Chen, X., Lei, L., Han, J., Jiang, Y., Ling, Z."Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells". Oncology Letters 15, no. 3 (2018): 3109-3120. https://doi.org/10.3892/ol.2017.7692
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