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Article Open Access

Gadd45β silencing impaired viability and metastatic phenotypes in cholangiocarcinoma cells by modulating the EMT pathway

  • Authors:
    • Kyaw Zwar Myint
    • Pornparn Kongpracha
    • Panthip Rattanasinganchan
    • Kawin Leelawat
    • Penpak Moolthiya
    • Kittipong Chaiyabutr
    • Rutaiwan Tohtong
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand, Department of Clinical Chemistry, Faculty of Medical Technology, Huachiew Chalermprakiet University, Bangkok 10400, Thailand, Department of Surgery, Rajavithi Hospital, Bangkok 10400, Thailand
    Copyright: © Myint et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3031-3041
    |
    Published online on: December 28, 2017
       https://doi.org/10.3892/ol.2017.7706
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Abstract

Growth arrest and DNA damage-inducible-β (Gadd45β) is a stress-response protein involved in a number of processes, including cell cycle control, DNA repair, survival and death control, and stress signaling, depending on its interactions. Gadd45β expression is dysregulated in numerous types of cancer, functioning as either a tumor promoter or a tumor suppressor. However, the functions of Gadd45β in cholangiocarcinoma (CCA), particularly in metastasis, has not been studied. The immunohistochemical analysis of Gadd45β expression revealed that 75% of histological specimens from patients with CCA expressed high levels of Gadd45β, and that high Gadd45β expression was associated with metastasis. The role of Gadd45β in CCA was examined using siRNA‑mediated gene knockdown in HuCCA‑1, a human CCA cell line established from a Thai patient. The effects of Gadd45β downregulation upon cell viability and death, invasion, migration, matrix metalloproteinase (MMP) activity and epithelial‑mesenchymal transition (EMT) marker expression were investigated. Gadd45β knockdown impaired cell viability, which was associated with the induction of apoptosis. In addition, there was a marked reduction in invasion and migration, although MMP activity was unaffected. Impairment of these metastatic properties was accompanied by the decreased expression of EMT markers, including Slug, vimentin, claudin‑1 and zona occludens protein 1, whereas E‑cadherin expression was increased. The present study suggests that Gadd45β is involved in regulating the viability and the metastatic potential of CCA cells, which may be mediated by the modulation of the EMT pathway.
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View References

1 

Blechacz B and Gores GJ: Cholangiocarcinoma: Advances in pathogenesis, diagnosis, and treatment. Hepatology. 48:308–321. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Welzel TM, McGlynn KA, Hsing AW, O'Brien TR and Pfeiffer RM: Impact of classification of hilar cholangiocarcinomas (Klatskin tumors) on the incidence of intra- and extrahepatic cholangiocarcinoma in the United States. J Natl Cancer Inst. 98:873–875. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Farley DR, Weaver AL and Nagorney DM: ‘Natural history’ of unresected cholangiocarcinoma: Patient outcome after noncurative intervention. Mayo Clin Proc. 70:pp. 425–429. 1995; View Article : Google Scholar : PubMed/NCBI

4 

Anderson CD, Pinson CW, Berlin J and Chari RS: Diagnosis and treatment of cholangiocarcinoma. Oncologist. 9:43–57. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Sripa B, Brindley PJ, Mulvenna J, Laha T, Smout MJ, Mairiang E, Bethony JM and Loukas A: The tumorigenic liver fluke Opisthorchis viverrini-multiple pathways to cancer. Trends Parasitol. 28:395–407. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Sripa B, Kaewkes S, Sithithaworn P, Mairiang E, Laha T, Smout M, Pairojkul C, Bhudhisawasdi V, Tesana S, Thinkamrop B, et al: Liver fluke induces cholangiocarcinoma. PLoS Med. 4:e2012007. View Article : Google Scholar : PubMed/NCBI

7 

Weigelt B, Peterse JL and van't Veer LJ: Breast cancer metastasis: Markers and models. Nat Rev Cancer. 5:591–602. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Heerboth S, Housman G, Leary M, Longacre M, Byler S, Lapinska K, Willbanks A and Sarkar S: EMT and tumor metastasis. Clin Transl Med. 4:62015. View Article : Google Scholar : PubMed/NCBI

9 

Nieto MA: Epithelial plasticity: A common theme in embryonic and cancer cells. Science. 342:12348502013. View Article : Google Scholar : PubMed/NCBI

10 

Moreno-Bueno G, Portillo F and Cano A: Transcriptional regulation of cell polarity in EMT and cancer. Oncogene. 27:6958–6969. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Peinado H, Olmeda D and Cano A: Snail, Zeb and bHLH factors in tumour progression: An alliance against the epithelial phenotype? Nat Rev Cancer. 7:415–428. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Adhikary A, Chakraborty S, Mazumdar M, Ghosh S, Mukherjee S, Manna A, Mohanty S, Nakka KK, Joshi S, De A, et al: Inhibition of epithelial to mesenchymal transition by E-cadherin up-regulation via repression of slug transcription and inhibition of E-cadherin degradation: Dual role of scaffold/matrix attachment region-binding protein 1 (SMAR1) in breast cancer cells. J Biol Chem. 289:25431–25444. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Techasen A, Loilome W, Namwat N, Khuntikeo N, Puapairoj A, Jearanaikoon P, Saya H and Yongvanit P: Loss of E-cadherin promotes migration and invasion of cholangiocarcinoma cells and serves as a potential marker of metastasis. Tumour Biol. 35:8645–8652. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Mendez MG, Kojima S and Goldman RD: Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. FASEB J. 24:1838–1851. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Chaw SY, Majeed AA, Dalley AJ, Chan A, Stein S and Farah CS: Epithelial to mesenchymal transition (EMT) biomarkers-E-cadherin, beta-catenin, APC and Vimentin-in oral squamous cell carcinogenesis and transformation. Oral Oncol. 48:997–1006. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Qiu W, David D, Zhou B, Chu PG, Zhang B, Wu M, Xiao J, Han T, Zhu Z, Wang T, et al: Down-regulation of growth arrest DNA damage-inducible gene 45beta expression is associated with human hepatocellular carcinoma. Am J Pathol. 162:1961–1974. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Zumbrun SD, Hoffman B and Liebermann DA: Distinct mechanisms are utilized to induce stress sensor gadd45b by different stress stimuli. J Cell Biochem. 108:1220–1231. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Liebermann DA and Hoffman B: Gadd45 in stress signaling. J Mol Signal. 3:152008. View Article : Google Scholar : PubMed/NCBI

19 

Vairapandi M, Balliet AG, Fornace AJ Jr, Hoffman B and Liebermann DA: The differentiation primary response gene MyD118, related to GADD45, encodes for a nuclear protein which interacts with PCNA and p21WAF1/CIP1. Oncogene. 12:2579–2594. 1996.PubMed/NCBI

20 

Smith ML, Ford JM, Hollander MC, Bortnick RA, Amundson SA, Seo YR, Deng CX, Hanawalt PC and Fornace AJ Jr: p53-mediated DNA repair responses to UV radiation: Studies of mouse cells lacking p53, p21, and/or gadd45 genes. Mol Cell Biol. 20:3705–3714. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Jónsson ZO and Hübscher U: Proliferating cell nuclear antigen: More than a clamp for DNA polymerases. Bioessays. 19:967–975. 1997. View Article : Google Scholar : PubMed/NCBI

22 

Kelman Z and Hurwitz J: Protein-PCNA interactions: A DNA-scanning mechanism? Trends Biochem Sci. 23:236–238. 1998. View Article : Google Scholar : PubMed/NCBI

23 

Leprivier G, Rotblat B, Khan D, Jan E and Sorensen PH: Stress-mediated translational control in cancer cells. Biochim Biophys Acta. 1849:845–860. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Qiu W, Zhou B, Zou H, Liu X, Chu PG, Lopez R, Shih J, Chung C and Yen Y: Hypermethylation of growth arrest DNA damage-inducible gene 45 beta promoter in human hepatocellular carcinoma. Am J Pathol. 165:1689–1699. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Wang L, Xiao X, Li D, Chi Y, Wei P, Wang Y, Ni S, Tan C, Zhou X and Du X: Abnormal expression of GADD45B in human colorectal carcinoma. J Transl Med. 10:2152012. View Article : Google Scholar : PubMed/NCBI

26 

Meir T, Dror R, Yu X, Qian J, Simon I, Pe'er J and Chowers I: Molecular characteristics of liver metastases from uveal melanoma. Invest Ophthalmol Vis Sci. 48:4890–4896. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Inowa T, Hishikawa K, Matsuzaki Y, Isagawa T, Takeuchi T, Aburatani H, Kitamura T and Fujita T: GADD45β determines chemoresistance and invasive growth of side population cells of human embryonic carcinoma. Stem Cells Int. 2010:7829672010. View Article : Google Scholar : PubMed/NCBI

28 

Sirisinha S, Tengchaisri T, Boonpucknavig S, Prempracha N, Ratanarapee S and Pausawasdi A: Establishment and characterization of a cholangiocarcinoma cell line from a Thai patient with intrahepatic bile duct cancer. Asian Pac J Allergy Immunol. 9:153–157. 1991.PubMed/NCBI

29 

Pongcharoen P, Jinawath A and Tohtong R: Silencing of CD44 by siRNA suppressed invasion, migration and adhesion to matrix, but not secretion of MMPs, of cholangiocarcinoma cells. Clin Exp Metastasis. 28:827–839. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Schneider CA, Rasband WS and Eliceiri KW: NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 9:671–675. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Li YH and Zhu C: A modified Boyden chamber assay for tumor cell transendothelial migration in vitro. Clin Exp Metastasis. 17:423–429. 1999. View Article : Google Scholar : PubMed/NCBI

32 

Uhlen M, Fagerberg L, Hallström BM, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Å, Kampf C, Sjöstedt E, Asplund A, et al: Proteomics. Tissue-based map of the human proteome. Science. 347:12604192015. View Article : Google Scholar : PubMed/NCBI

33 

Kerr JF, Wyllie AH and Currie AR: Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 26:239–257. 1972. View Article : Google Scholar : PubMed/NCBI

34 

Tewari M, Quan LT, O'Rourke K, Desnoyers S, Zeng Z, Beidler DR, Poirier GG, Salvesen GS and Dixit VM: Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell. 81:801–809. 1995. View Article : Google Scholar : PubMed/NCBI

35 

Kumar A, Rajendran V, Sethumadhavan R and Purohit R: Akt kinase pathway: A leading target in cancer research. ScientificWorldJournal. 2013:7561342013. View Article : Google Scholar : PubMed/NCBI

36 

Nebreda AR and Porras A: p38 MAP kinases: Beyond the stress response. Trends Biochem Sci. 25:257–260. 2000. View Article : Google Scholar : PubMed/NCBI

37 

Kyriakis JM and Avruch J: Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation. Physiol Rev. 81:807–869. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Erhardt P, Schremser EJ and Cooper GM: B-Raf inhibits programmed cell death downstream of cytochrome c release from mitochondria by activating the MEK/Erk pathway. Mol Cell Biol. 19:5308–5315. 1999. View Article : Google Scholar : PubMed/NCBI

39 

Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

40 

Talmadge JE and Fidler IJ: AACR centennial series: The biology of cancer metastasis: Historical perspective. Cancer Res. 70:5649–5669. 2010. View Article : Google Scholar : PubMed/NCBI

41 

Mehlen P and Puisieux A: Metastasis: A question of life or death. Nat Rev Cancer. 6:449–458. 2006. View Article : Google Scholar : PubMed/NCBI

42 

Davies KJ: The complex interaction of matrix metalloproteinases in the migration of cancer cells through breast tissue Stroma. Int J Breast Cancer. 2014:8390942014. View Article : Google Scholar : PubMed/NCBI

43 

Roomi MW, Monterrey JC, Kalinovsky T, Rath M and Niedzwiecki A: Patterns of MMP-2 and MMP-9 expression in human cancer cell lines. Oncol Rep. 21:1323–1333. 2009.PubMed/NCBI

44 

Micalizzi DS, Farabaugh SM and Ford HL: Epithelial-mesenchymal transition in cancer: Parallels between normal development and tumor progression. J Mammary Gland Biol Neoplasia. 15:117–134. 2010. View Article : Google Scholar : PubMed/NCBI

45 

Powell DR, Blasky AJ, Britt SG and Artinger KB: Riding the crest of the wave: Parallels between the neural crest and cancer in epithelial-to-mesenchymal transition and migration. Wiley Interdiscip Rev Syst Biol Med. 5:511–522. 2013. View Article : Google Scholar : PubMed/NCBI

46 

Wallerand H, Cai Y, Wainberg ZA, Garraway I, Lascombe I, Nicolle G, Thiery JP, Bittard H, Radvanyi F and Reiter RR: Phospho-Akt pathway activation and inhibition depends on N-cadherin or phospho-EGFR expression in invasive human bladder cancer cell lines. Urol Oncol. 28:180–188. 2010. View Article : Google Scholar : PubMed/NCBI

47 

Furuse M, Fujita K, Hiiragi T, Fujimoto K and Tsukita S: Claudin-1 and −2: Novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J Cell Biol. 141:1539–1550. 1998. View Article : Google Scholar : PubMed/NCBI

48 

Stevenson BR, Siliciano JD, Mooseker MS and Goodenough DA: Identification of ZO-1: A high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J Cell Biol. 103:755–766. 1986. View Article : Google Scholar : PubMed/NCBI

49 

Rørth P: Collective cell migration. Annu Rev Cell Dev Biol. 25:407–429. 2009. View Article : Google Scholar : PubMed/NCBI

50 

Gupta M, Gupta SK, Balliet AG, Hollander MC, Fornace AJ, Hoffman B and Liebermann DA: Hematopoietic cells from Gadd45a- and Gadd45b-deficient mice are sensitized to genotoxic-stress-induced apoptosis. Oncogene. 24:7170–7179. 2005. View Article : Google Scholar : PubMed/NCBI

51 

De Smaele E, Zazzeroni F, Papa S, Nguyen DU, Jin R, Jones J, Cong R and Franzoso G: Induction of gadd45beta by NF-kappaB downregulates pro-apoptotic JNK signalling. Nature. 414:308–313. 2001. View Article : Google Scholar : PubMed/NCBI

52 

Zazzeroni F, Papa S, Algeciras-Schimnich A, Alvarez K, Melis T, Bubici C, Majewski N, Hay N, De Smaele E, Peter ME and Franzoso G: Gadd45 beta mediates the protective effects of CD40 costimulation against Fas-induced apoptosis. Blood. 102:3270–3279. 2003. View Article : Google Scholar : PubMed/NCBI

53 

Yothaisong S, Dokduang H, Techasen A, Namwat N, Yongvanit P, Bhudhisawasdi V, Puapairoj A, Riggins GJ and Loilome W: Increased activation of PI3K/Akt signaling pathway is associated with cholangiocarcinoma metastasis and PI3K/mTOR inhibition presents a possible therapeutic strategy. Tumour Biol. 34:3637–3648. 2013. View Article : Google Scholar : PubMed/NCBI

54 

Salerno DM, Tront JS, Hoffman B and Liebermann DA: Gadd45a and Gadd45b modulate innate immune functions of granulocytes and macrophages by differential regulation of p38 and JNK signaling. J Cell Physiol. 227:3613–3620. 2012. View Article : Google Scholar : PubMed/NCBI

55 

Kodama S and Negishi M: Pregnane X receptor PXR activates the GADD45beta gene, eliciting the p38 MAPK signal and cell migration. J Biol Chem. 286:3570–3578. 2011. View Article : Google Scholar : PubMed/NCBI

56 

Medici D, Hay ED and Olsen BR: Snail and Slug promote epithelial-mesenchymal transition through beta-catenin-T-cell factor-4-dependent expression of transforming growth factor-beta3. Mol Biol Cell. 19:4875–4887. 2008. View Article : Google Scholar : PubMed/NCBI

57 

Vuoriluoto K, Haugen H, Kiviluoto S, Mpindi JP, Nevo J, Gjerdrum C, Tiron C, Lorens JB and Ivaska J: Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer. Oncogene. 30:1436–1448. 2011. View Article : Google Scholar : PubMed/NCBI

58 

Kim JY, Kim YM, Yang CH, Cho SK, Lee JW and Cho M: Functional regulation of Slug/Snail2 is dependent on GSK-3β-mediated phosphorylation. FEBS J. 279:2929–2939. 2012. View Article : Google Scholar : PubMed/NCBI

59 

Cross DA, Alessi DR, Vandenheede JR, McDowell HE, Hundal HS and Cohen P: The inhibition of glycogen synthase kinase-3 by insulin or insulin-like growth factor 1 in the rat skeletal muscle cell line L6 is blocked by wortmannin, but not by rapamycin: Evidence that wortmannin blocks activation of the mitogen-activated protein kinase pathway in L6 cells between Ras and Raf. Biochem J. 303:21–26. 1994. View Article : Google Scholar : PubMed/NCBI

60 

Bezdekova M, Brychtova S, Sedlakova E, Langova K, Brychta T and Belej K: Analysis of Snail-1, E-cadherin and claudin-1 expression in colorectal adenomas and carcinomas. Int J Mol Sci. 13:1632–1643. 2012. View Article : Google Scholar : PubMed/NCBI

61 

Stebbing J, Filipovic A and Giamas G: Claudin-1 as a promoter of EMT in hepatocellular carcinoma. Oncogene. 32:4871–4872. 2013. View Article : Google Scholar : PubMed/NCBI

62 

Oliveira SS and Morgado-Diaz JA: Claudins: Multifunctional players in epithelial tight junctions and their role in cancer. Cell Mol Life Sci. 64:17–28. 2007. View Article : Google Scholar : PubMed/NCBI

63 

Kwon MJ: Emerging roles of claudins in human cancer. Int J Mol Sci. 14:18148–18180. 2013. View Article : Google Scholar : PubMed/NCBI

64 

Bauer H, Zweimueller-Mayer J, Steinbacher P, Lametschwandtner A and Bauer HC: The dual role of zonula occludens (ZO) proteins. J Biomed Biotechnol. 2010:4025932010. View Article : Google Scholar : PubMed/NCBI

65 

Gottardi CJ, Arpin M, Fanning AS and Louvard D: The junction-associated protein, zonula occludens-1, localizes to the nucleus before the maturation and during the remodeling of cell-cell contacts. Proc Natl Acad Sci USA. 93:pp. 10779–10784. 1996; View Article : Google Scholar : PubMed/NCBI

66 

Smalley KS, Brafford P, Haass NK, Brandner JM, Brown E and Herlyn M: Up-regulated expression of zonula occludens protein-1 in human melanoma associates with N-cadherin and contributes to invasion and adhesion. Am J Pathol. 166:1541–1554. 2005. View Article : Google Scholar : PubMed/NCBI

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Myint KZ, Kongpracha P, Rattanasinganchan P, Leelawat K, Moolthiya P, Chaiyabutr K and Tohtong R: Gadd45β silencing impaired viability and metastatic phenotypes in cholangiocarcinoma cells by modulating the EMT pathway. Oncol Lett 15: 3031-3041, 2018.
APA
Myint, K.Z., Kongpracha, P., Rattanasinganchan, P., Leelawat, K., Moolthiya, P., Chaiyabutr, K., & Tohtong, R. (2018). Gadd45β silencing impaired viability and metastatic phenotypes in cholangiocarcinoma cells by modulating the EMT pathway. Oncology Letters, 15, 3031-3041. https://doi.org/10.3892/ol.2017.7706
MLA
Myint, K. Z., Kongpracha, P., Rattanasinganchan, P., Leelawat, K., Moolthiya, P., Chaiyabutr, K., Tohtong, R."Gadd45β silencing impaired viability and metastatic phenotypes in cholangiocarcinoma cells by modulating the EMT pathway". Oncology Letters 15.3 (2018): 3031-3041.
Chicago
Myint, K. Z., Kongpracha, P., Rattanasinganchan, P., Leelawat, K., Moolthiya, P., Chaiyabutr, K., Tohtong, R."Gadd45β silencing impaired viability and metastatic phenotypes in cholangiocarcinoma cells by modulating the EMT pathway". Oncology Letters 15, no. 3 (2018): 3031-3041. https://doi.org/10.3892/ol.2017.7706
Copy and paste a formatted citation
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Spandidos Publications style
Myint KZ, Kongpracha P, Rattanasinganchan P, Leelawat K, Moolthiya P, Chaiyabutr K and Tohtong R: Gadd45β silencing impaired viability and metastatic phenotypes in cholangiocarcinoma cells by modulating the EMT pathway. Oncol Lett 15: 3031-3041, 2018.
APA
Myint, K.Z., Kongpracha, P., Rattanasinganchan, P., Leelawat, K., Moolthiya, P., Chaiyabutr, K., & Tohtong, R. (2018). Gadd45β silencing impaired viability and metastatic phenotypes in cholangiocarcinoma cells by modulating the EMT pathway. Oncology Letters, 15, 3031-3041. https://doi.org/10.3892/ol.2017.7706
MLA
Myint, K. Z., Kongpracha, P., Rattanasinganchan, P., Leelawat, K., Moolthiya, P., Chaiyabutr, K., Tohtong, R."Gadd45β silencing impaired viability and metastatic phenotypes in cholangiocarcinoma cells by modulating the EMT pathway". Oncology Letters 15.3 (2018): 3031-3041.
Chicago
Myint, K. Z., Kongpracha, P., Rattanasinganchan, P., Leelawat, K., Moolthiya, P., Chaiyabutr, K., Tohtong, R."Gadd45β silencing impaired viability and metastatic phenotypes in cholangiocarcinoma cells by modulating the EMT pathway". Oncology Letters 15, no. 3 (2018): 3031-3041. https://doi.org/10.3892/ol.2017.7706
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