Effect of selective inhibition of aquaporin 1 on chemotherapy sensitivity of J82 human bladder cancer cells

Zhang,Xuefeng; Chen,Yun; Dong,Liming; Shi,Benkang

doi:10.3892/ol.2018.7727

Effect of selective inhibition of aquaporin 1 on chemotherapy sensitivity of J82 human bladder cancer cells

Authors:
- Xuefeng Zhang
- Yun Chen
- Liming Dong
- Benkang Shi
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Affiliations: Department of Urology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Urology, Weihai Central Hospital, Weihai, Shandong 264400, P.R. China
Published online on: January 4, 2018 https://doi.org/10.3892/ol.2018.7727
Pages: 3864-3869

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Abstract

The occurrence of resistance to mitomycin C (MMC) often limits its clinical effectiveness. Combination therapy thus is employed to overcome this treatment resistance. The present study aimed to establish a novel J82 bladder cancer cell line so as to study the effect of inhibition of aquaporin 1 (AQP‑1) on chemotherapy sensitivity of J82 bladder cancer cells. A novel J82 bladder cancer cell line whose expression of AQP‑1 is inhibited was established through transfection of J82 cells with newly constructed recombinant plasmid. The resulting cell line was designated J82‑short hairpin (sh)AQP1 and was subjected to further analyses together with J82 cell line. Reverse transcription‑polymerase chain reaction was used to quantify the expression of AQP‑1mRNA in the cells; cell viability was analyzed with MTT assay and apoptosis was measured by flow cytometry. The expression of cell proliferation and cell apoptosis‑associated proteins, proliferating cell nuclear antigen (PCNA), B cell lymphoma 2 (Bcl‑2), Bcl‑2 associated X protein (Bax) and caspase‑3, were detected by Western blot. A statistically significant decrease in the transcription and expression of AQP1 was observed in the J82‑shAQP1 cells as compared with J82 cells. J82‑shAQP1 cells treated by MMC, also had a lower cell viability than J82 cells treated by MMC and showed enhanced apoptosis. Western blot analysis revealed J82‑shAQP1 cells treated by MMC had less expression of PCNA, lower bcl‑2/Bax ratio and more expression of caspase‑3 as compared with the J82 cells treated by MMC. Selective inhibition of AQP‑1 enhanced MMC chemotherapy sensitivity of J82 bladder cancer cells, suggesting combination of AQP‑1 inhibition with MMC treatment as a promising treatment strategy to overcome bladder cancer treatment resistance.

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