Expression of matrix metalloproteinase-7 correlates with the invasion of T1 colorectal carcinoma

Urushibara,Fumihiko; Shiozawa,Eisuke; Miyachi,Hideyuki; Misawa,Masashi; Cho,Tomonari; Takehara,Yusuke; Arai,Nana; Funaki,Toshitaka; Tazawa,Sakiko; Homma,Mayumi; Norose,Tomoko; Omatsu,Mutsuko; Yamochi,Toshiko; Kunimura,Toshiaki; Tate,Genshu; Honda,Kazuho; Fumio,Ishida; Kudo,Shin‑Ei; Takimoto,Masafumi

doi:10.3892/ol.2018.7746

Expression of matrix metalloproteinase-7 correlates with the invasion of T1 colorectal carcinoma

Authors:
- Fumihiko Urushibara
- Eisuke Shiozawa
- Hideyuki Miyachi
- Masashi Misawa
- Tomonari Cho
- Yusuke Takehara
- Nana Arai
- Toshitaka Funaki
- Sakiko Tazawa
- Mayumi Homma
- Tomoko Norose
- Mutsuko Omatsu
- Toshiko Yamochi
- Toshiaki Kunimura
- Genshu Tate
- Kazuho Honda
- Ishida Fumio
- Shin‑Ei Kudo
- Masafumi Takimoto
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Affiliations: Department of Pathology, Showa University School of Medicine, Tokyo 142‑8555, Japan, Digestive Disease Center, Showa University Northern Yokohama Hospital, Tokyo 142‑8555, Japan, Department of Diagnostic Pathology, Showa University Northern Yokohama Hospital, Tokyo 142‑8555, Japan, Department of Anatomy, Showa University School of Medicine, Tokyo 142‑8555, Japan
Published online on: January 8, 2018 https://doi.org/10.3892/ol.2018.7746
Pages: 3614-3620
Copyright: © Urushibara et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

T1 colorectal carcinomas (CRCs) are an initial site of metastatic spread. Various risk factors for lymph node metastasis have been investigated in T1 CRCs. However, the major step in the entire process of metastasis remains unclear. In terms of carcinoma invasion and metastasis, matrix metalloproteinases (MMPs) have recently gained increasing attention. Notably, MMP‑7 is frequently overexpressed in CRCs, but its implication has not been determined in T1 CRCs yet. The present study aimed to clarify the associations between the pathological risk factors of T1 CRCs and MMP‑7. In the current study, 211 lesions of T1 CRC that were resected endoscopically or surgically at Showa University Northern Yokohama Hospital (Yokohama, Japan) between April 2008 and December 2009 were retrospectively analyzed. MMP‑7 was immunostained and evaluated by its frequency of expression. Pathological factors of T1 CRCs were analyzed in association with MMP‑7 expression. Furthermore, the ultrastructural alterations of carcinoma invasion were examined using low vacuum‑scanning electron microscopy (LV‑SEM). MMP‑7 expression was associated with venous invasion (P=0.005), and LV‑SEM revealed the disappearance of the normal structure of collagen and elastic fibers of veins invaded by tumor cells expressing MMP‑7. At the invasive front, MMP‑7 has a vital role in carcinoma invasion, correlating with venous invasion of T1 CRCs.

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