Open Access

Differential microRNA expression profiles in tamoxifen-resistant human breast cancer cell lines induced by two methods

  • Authors:
    • Peng Ye
    • Cheng Fang
    • Hui Zeng
    • Yu Shi
    • Zhongya Pan
    • Nairui An
    • Keli He
    • Li Zhang
    • Xinghua Long
  • View Affiliations

  • Published online on: January 10, 2018     https://doi.org/10.3892/ol.2018.7768
  • Pages: 3532-3539
  • Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tamoxifen (TAM) resistance has become a severe problem for endocrine therapy of breast cancer. The present study investigated the association between microRNA (miRNA) expression and TAM resistance in breast cancer. The TAM‑resistant breast cancer MCF-7C and MCF-7T cell lines were established using the human breast cancer cell line MCF‑7 as the parental cell line and 4-hydroxytamoxifen (OHT) as the screening drug in vitro. The MCF‑7C cell line was established by dose stepwise induction beginning with a low concentration of OHT; the MCF‑7T cell line was established by temporal stepwise induction beginning with a high concentration of OHT. Differential miRNA expression profiles between TAM‑sensitive (MCF‑7) and TAM‑resistant (MCF‑7C and MCF‑7T) breast cancer cell lines were detected and analyzed using RNA sequencing technology. The results of western blot analysis indicated that the level of ERα protein expression in drug‑resistant cells was significantly increased. A total of 1,646 miRNAs were detected in all samples, including 1,376 known miRNAs and 270 predicted miRNAs. There were 118 miRNAs expressed at significantly different levels between MCF‑7C and MCF‑7 cells (P<0.05); among them, 67 miRNAs were upregulated (P<0.05) and 51 miRNA were downregulated (P<0.05). There were 42 miRNAs expressed at significantly different levels between MCF‑7T and MCF‑7 (P<0.05); among them, 23 miRNAs were upregulated (P<0.05) and 19 miRNAs were downregulated (P<0.05). There were 126 miRNAs with significant differences between MCF‑7C and MCF‑7T (P<0.05); among them, 76 miRNAs were upregulated (P<0.05) and 50 miRNAs were downregulated. On the basis of the results of the present study, we hypothesize that miR‑21, miR‑146a, miR‑148a, miR‑34a and miR‑27a may serve important roles in mediating TAM resistance in breast cancer, and have potential as therapeutic targets for TAM-resistant breast cancer.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ye P, Fang C, Zeng H, Shi Y, Pan Z, An N, He K, Zhang L and Long X: Differential microRNA expression profiles in tamoxifen-resistant human breast cancer cell lines induced by two methods. Oncol Lett 15: 3532-3539, 2018
APA
Ye, P., Fang, C., Zeng, H., Shi, Y., Pan, Z., An, N. ... Long, X. (2018). Differential microRNA expression profiles in tamoxifen-resistant human breast cancer cell lines induced by two methods. Oncology Letters, 15, 3532-3539. https://doi.org/10.3892/ol.2018.7768
MLA
Ye, P., Fang, C., Zeng, H., Shi, Y., Pan, Z., An, N., He, K., Zhang, L., Long, X."Differential microRNA expression profiles in tamoxifen-resistant human breast cancer cell lines induced by two methods". Oncology Letters 15.3 (2018): 3532-3539.
Chicago
Ye, P., Fang, C., Zeng, H., Shi, Y., Pan, Z., An, N., He, K., Zhang, L., Long, X."Differential microRNA expression profiles in tamoxifen-resistant human breast cancer cell lines induced by two methods". Oncology Letters 15, no. 3 (2018): 3532-3539. https://doi.org/10.3892/ol.2018.7768