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Article

Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis

  • Authors:
    • Zhijun Zhao
    • Bin Wang
    • Junhai Hao
    • Weitao Man
    • Yongkai Chang
    • Shunchang Ma
    • Yeshuai Hu
    • Fusheng Liu
    • Jun Yang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Baotou Medical College, Baotou, Inner Mongolia 014010, P.R. China, Department of Neurosurgery, Fuxing Hospital, Capital Medical University, Beijing 100038, P.R. China, The Second Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Neurosurgery, Brain Tumor Research Center, Beijing Neurosurgical Institute, Beijing Tiantan Hospital Affiliated with The Capital Medical University, Beijing 100050, P.R. China, Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
  • Pages: 4026-4032
    |
    Published online on: January 12, 2018
       https://doi.org/10.3892/ol.2018.7784
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Abstract

Expression of the long non-coding RNA taurine-upregulated gene 1 (TUG1) is associated with various aggressive tumors. The present study aimed to investigate the biological function of TUG1 in regulating apoptosis, proliferation, invasion and cell cycle distribution in human glioma U251 cells. Lentivirus‑mediated TUG1‑specific microRNA was transfected into U251 cells to abrogate the expression of TUG1. Flow cytometry analysis was used to examine the cell cycle distribution and apoptosis of U251 cells. Cellular proliferation was examined using Cell Counting Kit‑8 (CCK‑8) assays and invasion was examined by Transwell assays. The apoptotic rate of cells in the TUG1‑knockdown group was significantly higher than in the negative control (NC) group (11.58 vs. 9.14%, P<0.01). CCK‑8 assay data demonstrated that the proliferative ability of cells within the TUG1‑knockdown group was lower compared with that of the NC group. A Transwell invasion assay was performed, which revealed that the number of invaded cells from the TUG1‑knockdown group was the less compared with that of the NC group. In addition, the G0/G1 phase population was significantly increased within the treated group (44.85 vs. 38.45%, P<0.01), as measured by flow cytometry. The present study demonstrated that the downregulation of TUG1 may inhibit proliferation and invasion, and promote glioma U251 cell apoptosis. In addition, knockdown of TUG1 may have an effect on cell cycle arrest. The data presented in the current study indicated that TUG1 may be a novel therapeutic target for glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Z, Wang B, Hao J, Man W, Chang Y, Ma S, Hu Y, Liu F and Yang J: Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis. Oncol Lett 15: 4026-4032, 2018.
APA
Zhao, Z., Wang, B., Hao, J., Man, W., Chang, Y., Ma, S. ... Yang, J. (2018). Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis. Oncology Letters, 15, 4026-4032. https://doi.org/10.3892/ol.2018.7784
MLA
Zhao, Z., Wang, B., Hao, J., Man, W., Chang, Y., Ma, S., Hu, Y., Liu, F., Yang, J."Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis". Oncology Letters 15.3 (2018): 4026-4032.
Chicago
Zhao, Z., Wang, B., Hao, J., Man, W., Chang, Y., Ma, S., Hu, Y., Liu, F., Yang, J."Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis". Oncology Letters 15, no. 3 (2018): 4026-4032. https://doi.org/10.3892/ol.2018.7784
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Z, Wang B, Hao J, Man W, Chang Y, Ma S, Hu Y, Liu F and Yang J: Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis. Oncol Lett 15: 4026-4032, 2018.
APA
Zhao, Z., Wang, B., Hao, J., Man, W., Chang, Y., Ma, S. ... Yang, J. (2018). Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis. Oncology Letters, 15, 4026-4032. https://doi.org/10.3892/ol.2018.7784
MLA
Zhao, Z., Wang, B., Hao, J., Man, W., Chang, Y., Ma, S., Hu, Y., Liu, F., Yang, J."Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis". Oncology Letters 15.3 (2018): 4026-4032.
Chicago
Zhao, Z., Wang, B., Hao, J., Man, W., Chang, Y., Ma, S., Hu, Y., Liu, F., Yang, J."Downregulation of the long non-coding RNA taurine-upregulated gene 1 inhibits glioma cell proliferation and invasion and promotes apoptosis". Oncology Letters 15, no. 3 (2018): 4026-4032. https://doi.org/10.3892/ol.2018.7784
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