Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
March-2018 Volume 15 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2018 Volume 15 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

MicroRNA-433 targets AKT3 and inhibits cell proliferation and viability in breast cancer

  • Authors:
    • Xiaolei Hu
    • Jie Wang
    • Wan He
    • Pan Zhao
    • Changsheng Ye
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Clinical Nutrition Department, The 458th Hospital of People's Liberation Army, Guangzhou, Guangdong 510620, P.R. China, Department of Oncology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, Guangdong 518000, P.R. China, Department of Clinical Medical Research, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, Guangdong 518000, P.R. China
  • Pages: 3998-4004
    |
    Published online on: January 16, 2018
       https://doi.org/10.3892/ol.2018.7803
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Breast cancer is the most frequently diagnosed malignancy in women. However, the molecular mechanisms underlying breast cancer pathogenesis are not fully understood. The present study examined the role of miR‑433 in breast cancer and investigated its underlying molecular mechanisms of action. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were performed to analyze the level of microRNA (miRNA/miR)/mRNA and protein expression, respectively. Additionally, MTT assay was used to determined cell proliferation and viability. Cell apoptosis was measured by flow cytometry. A dual‑luciferase reporter assay was used to confirm the identity of the downstream target of miR‑433. The results revealed that miR‑433 was downregulated in breast cancer tissues and cell lines. Overexpression of miR‑433 inhibited cell proliferation and cell viability in BT‑549 cells, whereas downregulation of miR‑433 increased cell proliferation and cell viability in MDA‑MB‑231 cells. Further flow cytometry analysis revealed that miR‑433 was able to induce apoptosis and also alter the levels of proteins expression of B‑cell lymphoma‑2 and Bcl‑associated X. Bioinformatics analysis showed that RAC‑γ serine/threonine‑protein kinase (AKT3) was one of the downstream targets of miR‑433, and luciferase reporter assay further confirmed that AKT3 is a direct target of miR‑433. The knockdown of AKT3 was able to inhibit proliferation and viability in BT‑549 cells. Overexpression of AKT3 prevented the inhibitory effects of miR‑433 on proliferation and viability in BT‑549 cells. The level of AKT3 mRNA expression was upregulated in breast cancer tissues compared with normal tissues and was inversely correlated with miR‑433 expression levels. In summary, the results of the present study results indicate that the tumor‑suppressive role of miR‑433 may be mediated by regulating AKT3. miR‑433 may therefore serve as a potential therapeutic target for breast cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Rudolph A, Chang-Claude J and Schmidt MK: Gene-environment interaction and risk of breast cancer. Br J Cancer. 114:125–133. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Li C, Yang L, Zhang D and Jiang W: Systematic review and meta-analysis suggest that dietary cholesterol intake increases risk of breast cancer. Nutr Res. 36:627–635. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Srivastava S, Reid BJ, Ghosh S and Kramer BS: Research needs for understanding the biology of overdiagnosis in cancer screening. J Cell Physiol. 231:1870–1875. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Golubnitschaja O, Debald M, Yeghiazaryan K, Kuhn W, Pešta M, Costigliola V and Grech G: Breast cancer epidemic in the early twenty-first century: Evaluation of risk factors, cumulative questionnaires and recommendations for preventive measures. Tumour Biol. 37:12941–12957. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Sonnenblick A, Pondé N and Piccart M: Metastatic breast cancer: The Odyssey of personalization. Mol Oncol. 10:1147–1159. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Tzanninis IG, Kotteas EA, Ntanasis-Stathopoulos I, Kontogianni P and Fotopoulos G: Management and outcomes in metaplastic breast cancer. Clin Breast Cancer. 16:437–443. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Hemmatzadeh M, Mohammadi H, Jadidi-Niaragh F, Asghari F and Yousefi M: The role of oncomirs in the pathogenesis and treatment of breast cancer. Biomed Pharmacother. 78:129–139. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Pinweha P, Rattanapornsompong K, Charoensawan V and Jitrapakdee S: MicroRNAs and oncogenic transcriptional regulatory networks controlling metabolic reprogramming in cancers. Comput Struct Biotechnol J. 14:223–233. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Leung AK: The Whereabouts of microRNA Actions: Cytoplasm and beyond. Trends Cell Biol. 25:601–610. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Li X, Yang L, Shuai T, Piao T and Wang R: MiR-433 inhibits retinoblastoma malignancy by suppressing Notch1 and PAX6 expression. Biomed Pharmacother. 82:247–255. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Weiner-Gorzel K, Dempsey E, Milewska M, McGoldrick A, Toh V, Walsh A, Lindsay S, Gubbins L, Cannon A, Sharpe D, et al: Overexpression of the microRNA miR-433 promotes resistance to paclitaxel through the induction of cellular senescence in ovarian cancer cells. Cancer Med. 4:745–758. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Wang XC, Ma Y, Meng PS, Han JL, Yu HY and Bi LJ: miR-433 inhibits oral squamous cell carcinoma (OSCC) cell growth and metastasis by targeting HDAC6. Oral Oncol. 51:674–682. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Guo LH, Li H, Wang F, Yu J and He JS: The tumor suppressor roles of miR-433 and miR-127 in gastric cancer. Int J Mol Sci. 14:14171–14184. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Tan Y, Ge G, Pan T, Wen D, Chen L, Yu X, Zhou X and Gan J: A serum microRNA panel as potential biomarkers for hepatocellular carcinoma related with hepatitis B virus. PLoS One. 9:e1079862014. View Article : Google Scholar : PubMed/NCBI

15 

Del Vescovo V, Meier T, Inga A, Denti MA and Borlak J: A cross-platform comparison of affymetrix and Agilent microarrays reveals discordant miRNA expression in lung tumors of c-Raf transgenic mice. PLoS One. 8:e788702013. View Article : Google Scholar : PubMed/NCBI

16 

Xu X, Zhu Y, Liang Z, Li S, Xu X, Wang X, Wu J, Hu Z, Meng S, Liu B, et al: c-Met and CREB1 are involved in miR-433-mediated inhibition of the epithelial-mesenchymal transition in bladder cancer by regulating Akt/GSK-3β/Snail signaling. Cell Death Dis. 7:e20882016. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Bellacosa A, Kumar CC, Di Cristofano A and Testa JR: Activation of AKT kinases in cancer: Implications for therapeutic targeting. Adv Cancer Res. 94:29–86. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Luo H, Zhang H, Zhang Z, Zhang X, Ning B, Guo J, Nie N, Liu B and Wu X: Down-regulated miR-9 and miR-433 in human gastric carcinoma. J Exp Clin Cancer Res. 28:822009. View Article : Google Scholar : PubMed/NCBI

20 

Wang K, Li L, Wu J, Qiu Q, Zhou F and Wu H: The different expression profiles of microRNAs in elderly and young human dental pulp and the role of miR-433 in human dental pulp cells. Mech Ageing Dev 146–148. 1–11. 2015. View Article : Google Scholar

21 

Dillon RL and Muller WJ: Distinct biological roles for the akt family in mammary tumor progression. Cancer Res. 70:4260–4264. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Chin YR and Toker A: Function of Akt/PKB signaling to cell motility, invasion and the tumor stroma in cancer. Cell Signal. 21:470–476. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Sultana A, Idress R, Naqvi ZA, Azam I, Khan S, Siddiqui AA and Lalani EN: Expression of the androgen receptor, pAkt, and pPTEN in breast cancer and their potential in prognostication. Transl Oncol. May 12–2014.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

24 

Chin YR, Yoshida T, Marusyk A, Beck AH, Polyak K and Toker A: Targeting Akt3 signaling in triple-negative breast cancer. Cancer Res. 74:964–973. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Grottke A, Ewald F, Lange T, Nörz D, Herzberger C, Bach J, Grabinski N, Gräser L, Höppner F, Nashan B, et al: Downregulation of AKT3 increases migration and metastasis in triple negative breast cancer cells by upregulating S100A4. PLoS One. 11:e01463702016. View Article : Google Scholar : PubMed/NCBI

26 

Mosquera JM, Varma S, Pauli C, MacDonald TY, Yashinskie JJ, Varga Z, Sboner A, Moch H, Rubin MA and Shin SJ: MAGI3-AKT3 fusion in breast cancer amended. Nature. 520:E11–E12. 2015. View Article : Google Scholar : PubMed/NCBI

27 

O'Hurley G, Daly E, O'Grady A, Cummins R, Quinn C, Flanagan L, Pierce A, Fan Y, Lynn MA, Rafferty M, et al: Investigation of molecular alterations of AKT-3 in triple-negative breast cancer. Histopathology. 64:660–670. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Stottrup C, Tsang T and Chin YR: Upregulation of AKT3 confers resistance to the AKT inhibitor MK2206 in breast cancer. Mol Cancer Ther. 15:1964–1974. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Waugh MG: Amplification of chromosome 1q genes encoding the phosphoinositide signalling enzymes PI4KB, AKT3, PIP5K1A and PI3KC2B in breast cancer. J Cancer. 5:790–796. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hu X, Wang J, He W, Zhao P and Ye C: MicroRNA-433 targets AKT3 and inhibits cell proliferation and viability in breast cancer. Oncol Lett 15: 3998-4004, 2018.
APA
Hu, X., Wang, J., He, W., Zhao, P., & Ye, C. (2018). MicroRNA-433 targets AKT3 and inhibits cell proliferation and viability in breast cancer. Oncology Letters, 15, 3998-4004. https://doi.org/10.3892/ol.2018.7803
MLA
Hu, X., Wang, J., He, W., Zhao, P., Ye, C."MicroRNA-433 targets AKT3 and inhibits cell proliferation and viability in breast cancer". Oncology Letters 15.3 (2018): 3998-4004.
Chicago
Hu, X., Wang, J., He, W., Zhao, P., Ye, C."MicroRNA-433 targets AKT3 and inhibits cell proliferation and viability in breast cancer". Oncology Letters 15, no. 3 (2018): 3998-4004. https://doi.org/10.3892/ol.2018.7803
Copy and paste a formatted citation
x
Spandidos Publications style
Hu X, Wang J, He W, Zhao P and Ye C: MicroRNA-433 targets AKT3 and inhibits cell proliferation and viability in breast cancer. Oncol Lett 15: 3998-4004, 2018.
APA
Hu, X., Wang, J., He, W., Zhao, P., & Ye, C. (2018). MicroRNA-433 targets AKT3 and inhibits cell proliferation and viability in breast cancer. Oncology Letters, 15, 3998-4004. https://doi.org/10.3892/ol.2018.7803
MLA
Hu, X., Wang, J., He, W., Zhao, P., Ye, C."MicroRNA-433 targets AKT3 and inhibits cell proliferation and viability in breast cancer". Oncology Letters 15.3 (2018): 3998-4004.
Chicago
Hu, X., Wang, J., He, W., Zhao, P., Ye, C."MicroRNA-433 targets AKT3 and inhibits cell proliferation and viability in breast cancer". Oncology Letters 15, no. 3 (2018): 3998-4004. https://doi.org/10.3892/ol.2018.7803
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team