Novel exostosin-2 mutation identified in a Chinese family with hereditary multiple osteochondroma

Ruan,Weiwei; Cao,Li; Chen,Zhonghua; Kong,Mingxiang; Bi,Qing

doi:10.3892/ol.2018.7838

Novel exostosin-2 mutation identified in a Chinese family with hereditary multiple osteochondroma

Authors:
- Weiwei Ruan
- Li Cao
- Zhonghua Chen
- Mingxiang Kong
- Qing Bi
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Affiliations: Department of Orthopedics, Tongde Hospital of Zhejiang Provincial, Hangzhou, Zhejiang 310012, P.R. China, Department of Orthopedics and Joint Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China
Published online on: January 22, 2018 https://doi.org/10.3892/ol.2018.7838
Pages: 4383-4389
Copyright: © Ruan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hereditary multiple osteochondroma (HMO) is an autosomal dominant genetic disorder characterized by multiple outgrowing bony tumors capped by cartilage, generally affecting the metaphyses. The disease is known as hereditary multiple exostoses, familial exostosis, multiple cartilaginous exostoses or hereditary malformation of cartilage. The prevalence of HMO in Europe and the Unites States is ~1:100,000, although it has not been reported in China. The disease is often accompanied by pain, asymmetry and skeletal malformations, including forearm and leg bending deformities, limb length discrepancies, and knee internal and external rotation abnormalities. Mutations to exostosin‑1 (EXT1) and EXT2 mutations cause insufficient heparan sulfate biosynthesis, leading to chondrocyte proliferation, abnormal bone growth in neighboring regions, multiple exostoses, and ultimately malignant transformation. The risk of malignant degeneration to osteochondrosarcoma increases with age, despite the low lifetime risk (~1%). The present study selected a clinical feature of typical HMO pedigrees, and examined mutations in family members by Sanger sequencing. Each of the five patients examined had a novel heterozygous nonsense mutation, c.67C>T p.Arg23*. The mutation is located prior to the EXT2 exostosin domains in the amino acid sequence and results in a protein truncation of the 705 C‑terminal amino acids. The present study provides molecular genetic evidence for a novel causal mechanism of HMO, and provides the basis for clinical genetic counseling for similar diseases.

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