Casticin inhibits the epithelial-mesenchymal transition in ovarian carcinoma via the hedgehog signaling pathway

  • Authors:
    • Jing Zhang
    • Yinghong Cui
    • Shuwen Sun
    • Jianguo Cao
    • Xiaoling Fang
  • View Affiliations

  • Published online on: January 26, 2018     https://doi.org/10.3892/ol.2018.7880
  • Pages: 4495-4502
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Abstract

Casticin inhibits migration, invasion and induced apoptosis in numerous cancer cells; however, the Hedgehog (Hh) signaling pathway is a key factor in the epithelial‑mesenchymal transition (EMT). The present study aimed to assess whether casticin affects the expression of members of the Hh signaling pathway and EMT effectors in ovarian carcinoma. The ovarian cancer SKOV3 cell line was incubated in the presence of various concentrations of casticin or cyclopamine. Next, the expression levels of the main Hh signaling effector glioma‑associated oncogene-1 (Gli‑1) and EMT‑associated factors [Twist‑related protein 1 (Twist1), E‑cadherin and N‑cadherin] were determined by western blotting and reverse transcription‑quantitative polymerase chain reaction. Cell proliferation and growth were assessed using MTT and soft agar assays; cell migration and invasion was evaluated using an in vitro migration assay and a transwell invasion assay, respectively. Compared with control group values, Gli‑1, Twist1 and N‑cadherin expression levels were reduced, whereas E‑cadherin levels were increased in the casticin‑ and cyclopamine‑treated groups. Incubation with casticin or cyclopamine resulted in markedly reduced SKOV3 cell viability, migration and invasion, in a dose‑dependent manner. To the best of our knowledge, the findings of the present study indicated for first time that casticin may inhibit EMT via Hh signaling in vitro, reducing the migratory ability of ovarian cancer cells.
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April-2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang J, Cui Y, Sun S, Cao J and Fang X: Casticin inhibits the epithelial-mesenchymal transition in ovarian carcinoma via the hedgehog signaling pathway. Oncol Lett 15: 4495-4502, 2018
APA
Zhang, J., Cui, Y., Sun, S., Cao, J., & Fang, X. (2018). Casticin inhibits the epithelial-mesenchymal transition in ovarian carcinoma via the hedgehog signaling pathway. Oncology Letters, 15, 4495-4502. https://doi.org/10.3892/ol.2018.7880
MLA
Zhang, J., Cui, Y., Sun, S., Cao, J., Fang, X."Casticin inhibits the epithelial-mesenchymal transition in ovarian carcinoma via the hedgehog signaling pathway". Oncology Letters 15.4 (2018): 4495-4502.
Chicago
Zhang, J., Cui, Y., Sun, S., Cao, J., Fang, X."Casticin inhibits the epithelial-mesenchymal transition in ovarian carcinoma via the hedgehog signaling pathway". Oncology Letters 15, no. 4 (2018): 4495-4502. https://doi.org/10.3892/ol.2018.7880