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Expression and correlation of CD44 and GP73 in cerebroma tissues

  • Authors:
    • Yuan Yuan
    • Lin Shi
    • Shijun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China, Department of Neurosurgery, Dezhou People's Hospital, Dezhou, Shandong 253014, P.R. China, Department of Emergency, Dezhou People's Hospital, Dezhou, Shandong 253014, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4958-4962
    |
    Published online on: January 29, 2018
       https://doi.org/10.3892/ol.2018.7904
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Abstract

Cerebroma, a nervous system tumor located in the cranial cavity, seriously affects health and may even become life-threatening. The aim of the study was to investigate and discuss the expression levels of cluster of differentiation 44 (CD44) and Golgi protein 73 (GP73) in different types of cerebroma tissues and to study the correlations of CD44 and GP73. Reverse transcription‑polymerase chain reaction (RT-PCR) was used to detect the mRNA expression levels of CD44 and GP73 in four kinds of cerebroma. The immunohistochemical streptavidin-biotin complex (SABC) method was applied to measure the expression levels of CD44 and GP73 in different types of cerebroma. Subsequently, immunofluorescence and western blot analysis were performed to detect the expression levels of CD44 and GP73 in four kinds of cerebroma tissues. Statistical Product and Service Solutions (SPSS) 17.0 software was used to analyze the differences in CD44 and GP73 expression levels of the four kinds of cerebroma tissues and normal brain tissues. Transcription of CD44 and GP73 mRNAs was detected in the four kinds of cerebroma tissues, and CD44 and GP73 proteins were expressed. The immunohisto­chemical results revealed that the expression levels of CD44 and GP73 in the four kinds of cerebroma were significantly increased compared with those in normal tissues. The immuno­fluorescence results indicated that both CD44 and GP73 were expressed in four kinds of cerebroma, and the expression of CD44 was higher than that of GP73. The results of analysis of variance showed that the differences in CD44 and GP73 expression levels in the four kinds of cerebroma tissues and normal brain tissues were statistically significant (P<0.01). The results show that the expression levels of CD44 and GP73 are obviously upregulated in four kinds of cerebroma tissues, suggesting that CD44 and GP73 have great value for cerebroma research and can provide a new direction for clinical study as well as the diagnosis and treatment of cerebroma.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan Y, Shi L and Wang S: Expression and correlation of CD44 and GP73 in cerebroma tissues. Oncol Lett 15: 4958-4962, 2018.
APA
Yuan, Y., Shi, L., & Wang, S. (2018). Expression and correlation of CD44 and GP73 in cerebroma tissues. Oncology Letters, 15, 4958-4962. https://doi.org/10.3892/ol.2018.7904
MLA
Yuan, Y., Shi, L., Wang, S."Expression and correlation of CD44 and GP73 in cerebroma tissues". Oncology Letters 15.4 (2018): 4958-4962.
Chicago
Yuan, Y., Shi, L., Wang, S."Expression and correlation of CD44 and GP73 in cerebroma tissues". Oncology Letters 15, no. 4 (2018): 4958-4962. https://doi.org/10.3892/ol.2018.7904
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan Y, Shi L and Wang S: Expression and correlation of CD44 and GP73 in cerebroma tissues. Oncol Lett 15: 4958-4962, 2018.
APA
Yuan, Y., Shi, L., & Wang, S. (2018). Expression and correlation of CD44 and GP73 in cerebroma tissues. Oncology Letters, 15, 4958-4962. https://doi.org/10.3892/ol.2018.7904
MLA
Yuan, Y., Shi, L., Wang, S."Expression and correlation of CD44 and GP73 in cerebroma tissues". Oncology Letters 15.4 (2018): 4958-4962.
Chicago
Yuan, Y., Shi, L., Wang, S."Expression and correlation of CD44 and GP73 in cerebroma tissues". Oncology Letters 15, no. 4 (2018): 4958-4962. https://doi.org/10.3892/ol.2018.7904
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