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Article

Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells

  • Authors:
    • Yang Guo
    • Ying Hu
    • Mingming Hu
    • Jiabei He
    • Baolan Li
  • View Affiliations / Copyright

    Affiliations: General Department Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, P.R. China
  • Pages: 5220-5226
    |
    Published online on: February 1, 2018
       https://doi.org/10.3892/ol.2018.7918
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Abstract

Lung cancer is among the leading causes of cancer‑associated mortality worldwide, with a low 5‑year survival rate of 16.1%. However, the underlying molecular mechanisms behind lung cancer tumorigenesis remain largely unknown. Long non‑coding RNAs (lncRNAs) have been demonstrated to serve a function in the tumorigenesis of multiple types of cancer. The objective of the present study was to identify the function of a newly identified lncRNA zinc finger E‑box‑binding homeobox 2 antisense RNA 1 (ZEB2‑AS1) in human lung cancer. Results demonstrated that the transcript level of ZEB2‑AS1 in human lung cancer was markedly upregulated in vivo and in vitro. The knockdown of ZEB2‑AS1 in A549 and NCI‑H292 cells, and the overexpression of ZEB2‑AS1 in H‑125 and H1975 cells, altered colony formation and cell proliferative rate, as examined using colony formation and cell proliferation assays. Western blot analysis revealed that the knockdown of ZEB2‑AS1 in A549 and NCI‑H292 cells increased the protein levels of B‑cell lymphoma 2 (Bcl‑2), Bcl-2-associated X protein (Bax), caspase‑3 and ‑9, upregulated the relative activities of caspase‑3 and ‑9, and had no observable effect on caspase‑8 activity. Similarly, the overexpression of ZEB2‑AS1 in H‑125 and H1975 cells resulted in decreased expression of caspase‑3, caspase‑9, Bcl‑2 and Bax. The results identified the effects of lncRNA ZEB2‑AS1 on lung cancer progression through promoting its proliferation and inhibiting cell apoptosis, indicating that ZEB2‑AS1 may serve as a novel prognostic factor for the diagnosis and treatment of human lung cancer in the clinic.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Y, Hu Y, Hu M, He J and Li B: Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells. Oncol Lett 15: 5220-5226, 2018.
APA
Guo, Y., Hu, Y., Hu, M., He, J., & Li, B. (2018). Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells. Oncology Letters, 15, 5220-5226. https://doi.org/10.3892/ol.2018.7918
MLA
Guo, Y., Hu, Y., Hu, M., He, J., Li, B."Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells". Oncology Letters 15.4 (2018): 5220-5226.
Chicago
Guo, Y., Hu, Y., Hu, M., He, J., Li, B."Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells". Oncology Letters 15, no. 4 (2018): 5220-5226. https://doi.org/10.3892/ol.2018.7918
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Y, Hu Y, Hu M, He J and Li B: Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells. Oncol Lett 15: 5220-5226, 2018.
APA
Guo, Y., Hu, Y., Hu, M., He, J., & Li, B. (2018). Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells. Oncology Letters, 15, 5220-5226. https://doi.org/10.3892/ol.2018.7918
MLA
Guo, Y., Hu, Y., Hu, M., He, J., Li, B."Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells". Oncology Letters 15.4 (2018): 5220-5226.
Chicago
Guo, Y., Hu, Y., Hu, M., He, J., Li, B."Long non-coding RNA ZEB2-AS1 promotes proliferation and inhibits apoptosis in human lung cancer cells". Oncology Letters 15, no. 4 (2018): 5220-5226. https://doi.org/10.3892/ol.2018.7918
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