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April-2018 Volume 15 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells

  • Authors:
    • Hui Di
    • Xinting Zhang
    • Yi Guo
    • Yanfang Shi
    • Chuan Fang
    • Yu Yuan
    • Jiwei Wang
    • Chao Shang
    • Wenzhe Guo
    • Chunhui Li

  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China, Department of Neurosurgery, Hebei University, Baoding, Hebei 071000, P.R. China, Department of Neurosurgery, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing 102218, P.R. China, Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin 300350, P.R. China
  • Pages: 5131-5136
    |
    Published online on: February 2, 2018
       https://doi.org/10.3892/ol.2018.7932
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Abstract

Glioblastoma multiforme (GBM) is a prevalent and aggressive disease, and the development of a novel therapy to better treat advanced GBM is urgently required. Lactate dehydrogenase A (LDHA), which functions as a key enzyme in transforming pyruvate into lactate, has attracted more attention in recent years due to its critical role in various types of advanced cancer. Previous data derived from the Oncomine database have shown that the expression of LDHA is higher in GBM tissues than that in corresponding normal control tissues. However, the association of LDHA levels with glioma clinical grades and the possible mechanisms of LDHA in GBM progression have not been investigated. The present study showed that there is a significant positive correlation between LDHA expression levels and tumor clinical stages. The knockdown of LDHA inhibited cell growth by inhibiting cell cycle progression and inducing apoptosis in glioma cell lines. Upon investigating the molecular mechanism, LDHA knockdown via siRNA treatment was associated with decreased cyclin D1 expression, increased cleavage of PARP, and altered B‑cell lymphoma 2 and B‑cell lymphoma 2‑associated protein X expression. In addition, LDHA knockdown led to the marked downregulation of matrix metalloproteinase (MMP)‑2, MMP‑9, VE‑Cadherin and vascular endothelial growth factor expression levels. Furthermore, knock down of LDHA enhanced the chemosensitivity of glioma cells to temozolomide (TMZ), a second‑generation alkylating agent with activity against recurrent high‑grade glioma. These findings support LDHA as a novel target for developing effective therapeutic strategies to treat GBM.
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Copy and paste a formatted citation
Spandidos Publications style
Di H, Zhang X, Guo Y, Shi Y, Fang C, Yuan Y, Wang J, Shang C, Guo W, Li C, Li C, et al: Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells. Oncol Lett 15: 5131-5136, 2018.
APA
Di, H., Zhang, X., Guo, Y., Shi, Y., Fang, C., Yuan, Y. ... Li, C. (2018). Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells. Oncology Letters, 15, 5131-5136. https://doi.org/10.3892/ol.2018.7932
MLA
Di, H., Zhang, X., Guo, Y., Shi, Y., Fang, C., Yuan, Y., Wang, J., Shang, C., Guo, W., Li, C."Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells". Oncology Letters 15.4 (2018): 5131-5136.
Chicago
Di, H., Zhang, X., Guo, Y., Shi, Y., Fang, C., Yuan, Y., Wang, J., Shang, C., Guo, W., Li, C."Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells". Oncology Letters 15, no. 4 (2018): 5131-5136. https://doi.org/10.3892/ol.2018.7932
Copy and paste a formatted citation
x
Spandidos Publications style
Di H, Zhang X, Guo Y, Shi Y, Fang C, Yuan Y, Wang J, Shang C, Guo W, Li C, Li C, et al: Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells. Oncol Lett 15: 5131-5136, 2018.
APA
Di, H., Zhang, X., Guo, Y., Shi, Y., Fang, C., Yuan, Y. ... Li, C. (2018). Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells. Oncology Letters, 15, 5131-5136. https://doi.org/10.3892/ol.2018.7932
MLA
Di, H., Zhang, X., Guo, Y., Shi, Y., Fang, C., Yuan, Y., Wang, J., Shang, C., Guo, W., Li, C."Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells". Oncology Letters 15.4 (2018): 5131-5136.
Chicago
Di, H., Zhang, X., Guo, Y., Shi, Y., Fang, C., Yuan, Y., Wang, J., Shang, C., Guo, W., Li, C."Silencing LDHA inhibits proliferation, induces apoptosis and increases chemosensitivity to temozolomide in glioma cells". Oncology Letters 15, no. 4 (2018): 5131-5136. https://doi.org/10.3892/ol.2018.7932
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