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Article

Andrographolide inhibits proliferation and induces cell cycle arrest and apoptosis in human melanoma cells

  • Authors:
    • Guo Liu
    • Haihan Chu
  • View Affiliations / Copyright

    Affiliations: Department of Burns and Plastic Surgery, Jining First People's Hospital, Jining, Shandong 272011, P.R. China
  • Pages: 5301-5305
    |
    Published online on: February 2, 2018
       https://doi.org/10.3892/ol.2018.7941
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Abstract

Andrographolide (Andro), a natural compound isolated from Andrographis paniculata, has been demonstrated to have anticancer efficacy in several types of tumors. In the present study, the anticancer effects and mechanism of Andro in human malignant melanoma were investigated. Cell viability analysis was performed using an MTT assay and the effect of Andro on the cell cycle and apoptosis of human malignant melanoma cells was determined by flow cytometry. Western blot analysis was performed to evaluate the protein expression levels of human malignant melanoma cells following treatment with Andro. The results revealed that Andro potently inhibited cell proliferation by inducing G2/M cell‑cycle arrest in human malignant melanoma C8161 and A375 cell lines. In addition, treatment with Andro induced apoptosis, which was associated with the cleavage of poly(adenosine diphosphate‑ribose) polymerase and activation of caspase‑3. It was observed that Andro induced activation of the c‑Jun N‑terminal kinase and p38 signaling pathway, which may be connected with cell cycle arrest and apoptosis. In conclusion, the results demonstrated that Andro may be a promising and effective agent for antitumor therapy against human malignant melanoma.
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1 

Liu J, Gu J, Feng Z, Yang Y, Zhu N, Lu W and Qi F: Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells. J Transl Med. 14:72016. View Article : Google Scholar : PubMed/NCBI

2 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Kircher DA, Silvis MR, Cho JH and Holmen SL: Melanoma brain metastasis: Mechanisms, models and medicine. Int J Mol Sci. 17:E14682016. View Article : Google Scholar : PubMed/NCBI

4 

Lincoln R, Kohler L, Monro S, Yin H, Stephenson M, Zong R, Chouai A, Dorsey C, Hennigar R, Thummel RP and McFarland SA: Exploitation of long-lived 3IL excited states for metal-organic photodynamic therapy: Verification in a metastatic melanoma model. J Am Chem Soc. 135:17161–17175. 2013. View Article : Google Scholar : PubMed/NCBI

5 

George DD, Armenio VA and Katz SC: Combinatorial immunotherapy for melanoma. Cancer Gene Ther. 24:141–147. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Asmar R, Yang J and Carvajal RD: Clinical utility of nivolumab in the treatment of advanced melanoma. Ther Clin Risk Manag. 12:313–325. 2016.PubMed/NCBI

7 

Wu KJ, Huang JM, Zhong HJ, Dong ZZ, Vellaisamy K, Lu JJ, Chen XP, Chiu P, Kwong DWJ, Han QB, et al: A natural product-like JAK2/STAT3 inhibitor induces apoptosis of malignant melanoma cells. PLoS One. 12:e01771232017. View Article : Google Scholar : PubMed/NCBI

8 

Zhang T, Li J, Yin F, Lin B, Wang Z, Xu J, Wang H, Zuo D, Wang G, Hua Y and Cai Z: Toosendanin demonstrates promising antitumor efficacy in osteosarcoma by targeting STAT3. Oncogene. 36:6627–6639. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Wang G, Zhang T, Sun W, Wang H, Yin F, Wang Z, Zuo D, Sun M, Zhou Z, Lin B, et al: Arsenic sulfide induces apoptosis and autophagy through the activation of ROS/JNK and suppression of Akt/mTOR signaling pathways in osteosarcoma. Free Radic Biol Med. 106:24–37. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Pan Z, Shao HX, Liu T, Lu XY and Fan XH: Research progress of 5-hydroxymethylfurfural, a safety-related substance in traditional Chinese medicine injections. Zhongguo Zhong Yao Za Zhi. 42:1842–1846. 2017.(In Chinese). PubMed/NCBI

11 

Song J, Zhong RL, Xia Z, Wu H, Zhong QX, Zhang ZH, Wei YJ, Shi ZQ, Feng L and Jia XB: Research and application of hepatotoxicity evaluation technique of traditional Chinese medicine. Zhongguo Zhong Yao Za Zhi. 42:41–48. 2017.(In Chinese). PubMed/NCBI

12 

Chakravarti RN and Chakravarti D: Andrographolide, the active constituent of Andrographis paniculata Nees; a preliminary communication. Ind Med Gaz. 86:96–97. 1951.PubMed/NCBI

13 

Jotwani R, Eswaran SV, Moonga S and Cutler CW: MMP-9/TIMP-1 imbalance induced in human dendritic cells by Porphyromonas gingivalis. FEMS Immunol Med Microbiol. 58:314–321. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Mishra SK, Tripathi S, Shukla A, Oh SH and Kim HM: Andrographolide and analogues in cancer prevention. Front Biosci (Elite Ed). 7:255–266. 2015.PubMed/NCBI

15 

Seo JY, Pyo E, An JP, Kim J, Sung SH and Oh WK: Andrographolide activates Keap1/Nrf2/ARE/HO-1 pathway in HT22 cells and suppresses microglial activation by Aβ42 through Nrf2-related inflammatory response. Mediators Inflamm. 2017:59061892017. View Article : Google Scholar : PubMed/NCBI

16 

Dai L, Wang G and Pan W: Andrographolide inhibits proliferation and metastasis of SGC7901 gastric cancer cells. Biomed Res Int. 2017:62421032017. View Article : Google Scholar : PubMed/NCBI

17 

Zhang HT, Yang J, Liang GH, Gao XJ, Sang Y, Gui T, Liang ZJ, Tam MS and Zha ZG: Andrographolide induces cell cycle arrest and apoptosis of chondrosarcoma by targeting TCF-1/SOX9 axis. J Cell Biochem. 118:4575–4586. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Wang W, Guo W, Li L, Fu Z, Liu W, Gao J, Shu Y, Xu Q, Sun Y and Gu Y: Andrographolide reversed 5-FU resistance in human colorectal cancer by elevating BAX expression. Biochem Pharmacol. 121:8–17. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Dong F, Han J, Jing G, Chen X, Yan S, Yue L, Cao Z, Liu X, Ma G and Liu J: Dihydroartemisinin transiently activates the JNK/SAPK signaling pathway in endothelial cells. Oncol Lett. 12:4699–4704. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Kumar A, Singh UK, Kini SG, Garg V, Agrawal S, Tomar PK, Pathak P, Chaudhary A, Gupta P and Malik A: JNK pathway signaling: A novel and smarter therapeutic targets for various biological diseases. Future Med Chem. 7:2065–2086. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Berwick M, Buller DB, Cust A, Gallagher R, Lee TK, Meyskens F, Pandey S, Thomas NE, Veierød MB and Ward S: Melanoma epidemiology and prevention. Cancer Treat Res. 167:17–49. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Berwick M, Erdei E and Hay J: Melanoma epidemiology and public health. Dermatol Clin. 27:205–214. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Higgins HW II, Lee KC, Galan A and Leffell DJ: Melanoma in situ: Part I. Epidemiology, screening, and clinical features. J Am Acad Dermatol. 73:181–192. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Lasithiotakis KG, Petrakis IE and Garbe C: Cutaneous melanoma in the elderly: Epidemiology, prognosis and treatment. Melanoma Res. 20:163–170. 2010.PubMed/NCBI

25 

Cheung HY, Cheung SH, Li J, Cheung CS, Lai WP, Fong WF and Leung FM: Andrographolide isolated from Andrographis paniculata induces cell cycle arrest and mitochondrial-mediated apoptosis in human leukemic HL-60 cells. Planta Med. 71:1106–1111. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Zang QQ, Zhang L, Gao N and Huang C: Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. J Integr Med. 14:51–59. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Li HY, Zhang J, Sun LL, Li BH, Gao HL, Xie T, Zhang N and Ye ZM: Celastrol induces apoptosis and autophagy via the ROS/JNK signaling pathway in human osteosarcoma cells: An in vitro and in vivo study. Cell Death Dis. 6:e16042015. View Article : Google Scholar : PubMed/NCBI

28 

Stark GR and Taylor WR: Analyzing the G2/M checkpoint. Methods Mol Biol. 280:51–82. 2004.PubMed/NCBI

29 

Dothager RS, Putt KS, Allen BJ, Leslie BJ, Nesterenko V and Hergenrother PJ: Synthesis and identification of small molecules that potently induce apoptosis in melanoma cells through G1 cell cycle arrest. J Am Chem Soc. 127:8686–8696. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Elmore S: Apoptosis: A review of programmed cell death. Toxicol Pathol. 35:495–516. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Ouyang L, Shi Z, Zhao S, Wang FT, Zhou TT, Liu B and Bao JK: Programmed cell death pathways in cancer: A review of apoptosis, autophagy and programmed necrosis. Cell Prolif. 45:487–498. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Zhang X, Chen Y, Jenkins LW, Kochanek PM and Clark RS: Bench-to-bedside review: Apoptosis/programmed cell death triggered by traumatic brain injury. Crit Care. 9:66–75. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Mondal J, Samadder A and Khuda-Bukhsh AR: Psorinum 6 × triggers apoptosis signals in human lung cancer cells. J Integr Med. 14:143–153. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Jia J, Yang F, Yang M, Wang C and Song Y: P38/JNK signaling pathway mediates the fluoride-induced down-regulation of Fam83h. Biochem Biophys Res Commun. 471:386–390. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Goc A, Al-Husein B, Kochuparambil ST, Liu J, Heston WW and Somanath PR: PI3 kinase integrates Akt and MAP kinase signaling pathways in the regulation of prostate cancer. Int J Oncol. 38:267–277. 2011.PubMed/NCBI

36 

Kim EK and Choi EJ: Pathological roles of MAPK signaling pathways in human diseases. Biochim Biophys Acta. 1802:396–405. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu G and Chu H: Andrographolide inhibits proliferation and induces cell cycle arrest and apoptosis in human melanoma cells. Oncol Lett 15: 5301-5305, 2018.
APA
Liu, G., & Chu, H. (2018). Andrographolide inhibits proliferation and induces cell cycle arrest and apoptosis in human melanoma cells. Oncology Letters, 15, 5301-5305. https://doi.org/10.3892/ol.2018.7941
MLA
Liu, G., Chu, H."Andrographolide inhibits proliferation and induces cell cycle arrest and apoptosis in human melanoma cells". Oncology Letters 15.4 (2018): 5301-5305.
Chicago
Liu, G., Chu, H."Andrographolide inhibits proliferation and induces cell cycle arrest and apoptosis in human melanoma cells". Oncology Letters 15, no. 4 (2018): 5301-5305. https://doi.org/10.3892/ol.2018.7941
Copy and paste a formatted citation
x
Spandidos Publications style
Liu G and Chu H: Andrographolide inhibits proliferation and induces cell cycle arrest and apoptosis in human melanoma cells. Oncol Lett 15: 5301-5305, 2018.
APA
Liu, G., & Chu, H. (2018). Andrographolide inhibits proliferation and induces cell cycle arrest and apoptosis in human melanoma cells. Oncology Letters, 15, 5301-5305. https://doi.org/10.3892/ol.2018.7941
MLA
Liu, G., Chu, H."Andrographolide inhibits proliferation and induces cell cycle arrest and apoptosis in human melanoma cells". Oncology Letters 15.4 (2018): 5301-5305.
Chicago
Liu, G., Chu, H."Andrographolide inhibits proliferation and induces cell cycle arrest and apoptosis in human melanoma cells". Oncology Letters 15, no. 4 (2018): 5301-5305. https://doi.org/10.3892/ol.2018.7941
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