Identification of side population cells in human lung adenocarcinoma A549 cell line and elucidation of the underlying roles in lung cancer

Xie,Tong; Mo,Lingzhao; Li,Li; Mao,Naiquan; Li,Danrong; Liu,Deseng; Zuo,Chuantian; Huang,Dingming; Pan,Qi; Yang,Li; Wang,Shoufeng

doi:10.3892/ol.2018.7956

Identification of side population cells in human lung adenocarcinoma A549 cell line and elucidation of the underlying roles in lung cancer

Authors:
- Tong Xie
- Lingzhao Mo
- Li Li
- Naiquan Mao
- Danrong Li
- Deseng Liu
- Chuantian Zuo
- Dingming Huang
- Qi Pan
- Li Yang
- Shoufeng Wang
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Affiliations: Department of Thoracic Surgery, The Tumor Hospital Affiliated to Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Gynecologic Oncology, The Tumor Hospital Affiliated to Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Experimental Centre, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Published online on: February 5, 2018 https://doi.org/10.3892/ol.2018.7956
Pages: 4900-4906
Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to isolate and characterize side population (SP) cells in the human lung cancer A549 cell line, and elucidate the molecular mechanism of SP cells underlying lung cancer. The SP and non‑SP (NSP) cells in A549 cells were isolated and their differentiation was analyzed by fluorescence‑activated cell sorting. An in vitro plate clone assay, Matrigel® Transwell assay and chemoresistance analysis of the sorted SP and NSP cells were performed. In addition, the sorted SP and NSP cells were injected into BALB/c nude mice to detect their tumorigenic potential in vivo. The expression of ATP‑binding cassette sub‑family G member 2 (ABCG2) in transplanted tumors was detected by immunohistochemistry. The SP and NSP cells were successfully isolated. The results demonstrated that SP cells accounted for 1.09% of live A549 cells. SP cells produced SP and NSP cells, while NSP cells only produced NSP cells. In addition, SP cells formed more colonies, exhibited improved invasive ability and increased levels of chemoresistance compared with NSP cells in vitro. SP cells demonstrated a higher tumorigenic potential in BALB/c nude mice, and the number of ABCG2‑positive cells in the SP xenograft tumors were significantly increased compared with that in the NSP xenograft tumors. The present study indicated that SP cells isolated from the human lung cancer A549 cell line demonstrated increased tumorigenicity, and improved invasive ability and chemoresistance compared with NSP cells. In addition, detection of ABCG2 expression may assist in predicting the chemotherapeutic outcome of patients, and serve as a target for treating lung cancer.

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