Suppression of Jab1 expression inhibits proliferation and promotes apoptosis of AMC-HN-8 cells

Li,Pei‑Hua; Wang,Lin; Pan,Yao‑Jie; Sang,Miao‑Miao; Zheng,Jun‑Nian; Pei,Dong‑Sheng

doi:10.3892/ol.2018.7963

Suppression of Jab1 expression inhibits proliferation and promotes apoptosis of AMC-HN-8 cells

Authors:
- Pei‑Hua Li
- Lin Wang
- Yao‑Jie Pan
- Miao‑Miao Sang
- Jun‑Nian Zheng
- Dong‑Sheng Pei
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Affiliations: Department of Otorhinolaryngology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Department of Rehabilitation Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China, Department of Pathology, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China
Published online on: February 6, 2018 https://doi.org/10.3892/ol.2018.7963
Pages: 5137-5142

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Abstract

c-Jun activation domain-binding protein‑1 (Jab1) is a multifunctional protein involved in cell proliferation and apoptosis, DNA damage and repair and genome stability. In a number of types of human carcinoma, the abnormal expression of Jab1 is associated with poor prognosis, suggesting that Jab1 serves a vital function in tumorigenesis. However, the functional effects and the underlying molecular mechanisms of Jab1 in laryngeal squamous cell carcinoma (LSCC) progression remain poorly understood. The results of the present study demonstrate that downregulating Jab1 expression promotes LSCC apoptosis while inhibiting the proliferation of LSCC cells. Furthermore, Jab1 inhibition results in decreased protein kinase B phosphorylation accompanied by increased caspase‑3 cleavage and p53 expression. It has been identified that the increased expression of Jab1 is markedly associated with LSCC progression, therefore Jab1 may be used as a novel target for the treatment of laryngeal cancer.

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1	Kapelari B, Bech-Otschir D, Hegerl R, Schade R, Dumdey R and Dubiel W: Electron microscopy and subunit-subunit interaction studies reveal a first architecture of COP9 signalosome. J Mol Biol. 300:1169–1178. 2000. View Article : Google Scholar : PubMed/NCBI
2	Wei N, Serino G and Deng XW: The COP9 signalosome: More than a protease. Trends Biochem Sci. 33:592–600. 2008. View Article : Google Scholar : PubMed/NCBI
3	Schwechheimer C and Deng XW: COP9 signalosome revisited: A novel mediator of protein degradation. Trends Cell Biol. 11:420–426. 2001. View Article : Google Scholar : PubMed/NCBI
4	Cope GA, Suh GS, Aravind L, Schwarz SE, Zipursky SL, Koonin EV and Deshaies RJ: Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of Nedd8 from Cul1. Science. 298:608–611. 2002. View Article : Google Scholar : PubMed/NCBI
5	Wei N and Deng XW: The COP9 signalosome. Annu Rev Cell Dev Biol. 19:261–286. 2003. View Article : Google Scholar : PubMed/NCBI
6	Claret FX, Hibi M, Dhut S, Toda T and Karin M: A new group of conserved coactivators that increase the specificity of AP-1 transcription factors. Nature. 383:453–457. 1996. View Article : Google Scholar : PubMed/NCBI
7	Chamovitz DA and Segal D: JAB1/CSN5 and the COP9 signalosome. A complex situation. EMBO Rep. 2:96–101. 2001. View Article : Google Scholar : PubMed/NCBI
8	Kwok SF, Solano R, Tsuge T, Chamovitz DA, Ecker JR, Matsui M and Deng XW: Arabidopsis homologs of a c-Jun coactivator are present both in monomeric form and in the COP9 complex, and their abundance is differentially affected by the pleiotropic cop/det/fus mutations. Plant Cell. 10:1779–1790. 1998. View Article : Google Scholar : PubMed/NCBI
9	Shackleford TJ and Claret FX: JAB1/CSN5: A new player in cell cycle control and cancer. Cell Div. 5:262010. View Article : Google Scholar : PubMed/NCBI
10	Winner M, Koong AC, Rendon BE, Zundel W and Mitchell RA: Amplification of tumor hypoxic responses by macrophage migration inhibitory factor-dependent hypoxia-inducible factor stabilization. Cancer Res. 67:186–193. 2007. View Article : Google Scholar : PubMed/NCBI
11	Oh W, Lee EW, Sung YH, Yang MR, Ghim J, Lee HW and Song J: Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem. 281:17457–17465. 2006. View Article : Google Scholar : PubMed/NCBI
12	Wan M and Cao X, Wu Y, Bai S, Wu L, Shi X, Wang N and Cao X: Jab1 antagonizes TGF-beta signaling by inducing Smad4 degradation. EMBO Rep. 3:171–176. 2002. View Article : Google Scholar : PubMed/NCBI
13	Tomoda K, Kubota Y, Arata Y, Mori S, Maeda M, Tanaka T, Yoshida M, Yoneda-Kato N and Kato JY: The cytoplasmic shuttling and subsequent degradation of p27Kip1 mediated by Jab1/CSN5 and the COP9 signalosome complex. J Biol Chem. 277:2302–2310. 2002. View Article : Google Scholar : PubMed/NCBI
14	Kato JY and Yoneda-Kato N: Mammalian COP9 signalosome. Genes Cells. 14:1209–1225. 2009. View Article : Google Scholar : PubMed/NCBI
15	Hsu MC, Huang CC, Chang HC, Hu TH and Hung WC: Overexpression of Jab1 in hepatocellular carcinoma and its inhibition by peroxisome proliferator-activated receptor{gamma} ligands in vitro and in vivo. Clin Cancer Res. 14:4045–4052. 2008. View Article : Google Scholar : PubMed/NCBI
16	Ahn J, Hong SA, Lee SE, Kim J, Oh YS, Park SJ and Chung YJ: Cytoplasmic localization of Jab1 and p27 Kip1 might be associated with invasiveness of papillary thyroid carcinoma. Endocr J. 56:707–713. 2009. View Article : Google Scholar : PubMed/NCBI
17	Jemal A, Siegel R, Ward E, Hao Y, Xu J and Thun MJ: Cancer statistics, 2009. CA Cancer J Clin. 59:225–249. 2009. View Article : Google Scholar : PubMed/NCBI
18	Liu XK, Li Q, Xu LH, Hu LJ, Liao WG, Zhang XR, Liu ZM, Wu D and Zeng MS: Expression and clinical significance of SIAH in laryngeal squamous cell carcinoma. Med Oncol. 30:4852013. View Article : Google Scholar : PubMed/NCBI
19	Boyle P and Ferlay J: Cancer incidence and mortality in Europe, 2004. Ann Oncol. 16:481–488. 2005. View Article : Google Scholar : PubMed/NCBI
20	Ferlay J, Parkin DM and Steliarova-Foucher E: Estimates of cancer incidence and mortality in Europe in 2008. Eur J Cancer. 46:765–781. 2010. View Article : Google Scholar : PubMed/NCBI
21	Li JJ, Yang XM, Wang SH and Tang QL: Prognostic role of epidermal growth factor-like domain 7 protein expression in laryngeal squamous cell carcinoma. J Laryngol Otol. 125:1152–1157. 2011. View Article : Google Scholar : PubMed/NCBI
22	Schütz AK, Hennes T, Jumpertz S, Fuchs S and Bernhagen J: Role of CSN5/JAB1 in Wnt/β-catenin activation in colorectal cancer cells. FEBS Lett. 586:1645–1651. 2012. View Article : Google Scholar : PubMed/NCBI
23	Lee YH, Judge AD, Seo D, Kitade M, Gómez-Quiroz LE, Ishikawa T, Andersen JB, Kim BK, Marquardt JU, Raggi C, et al: Molecular targeting of CSN5 in human hepatocellular carcinoma: A mechanism of therapeutic response. Oncogene. 30:4175–4184. 2011. View Article : Google Scholar : PubMed/NCBI
24	Dong Y, Sui L, Watanabe Y, Yamaguchi F, Hatano N and Tokuda M: Prognostic significance of Jab1 expression in laryngeal squamous cell carcinomas. Clin Cancer Res. 11:259–266. 2005.PubMed/NCBI
25	Tomoda K, Kubota Y and Kato J: Degradation of the cyclin-dependent-kinase inhibitor p27Kip1 is instigated by Jab1. Nature. 398:160–165. 1999. View Article : Google Scholar : PubMed/NCBI
26	Adler AS, Littlepage LE, Lin M, Kawahara TL, Wong DJ, Werb Z and Chang HY: CSN5 isopeptidase activity links COP9 signalosome activation to breast cancer progression. Cancer Res. 68:506–515. 2008. View Article : Google Scholar : PubMed/NCBI
27	Thompson CB: Apoptosis in the pathogenesis and treatment of disease. Science. 267:1456–1462. 1995. View Article : Google Scholar : PubMed/NCBI
28	Porter AG and Jänicke RU: Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 6:99–104. 1999. View Article : Google Scholar : PubMed/NCBI
29	Shen Y and White E: p53-dependent apoptosis pathways. Adv Cancer Res. 82:55–84. 2001. View Article : Google Scholar : PubMed/NCBI
30	Abu-Qare AW and Abou-Donia MB: Biomarkers of apoptosis: Release of cytochrome c, activation of caspase-3, induction of 8-hydroxy-2′-deoxyguanosine, increased 3-nitrotyrosine, and alteration of p53 gene. J Toxicol Environ Health B Crit Rev. 4:313–332. 2001. View Article : Google Scholar : PubMed/NCBI
31	Cicenas J: The potential role of Akt phosphorylation in human cancers. Int J Biol Markers. 23:1–9. 2008. View Article : Google Scholar : PubMed/NCBI