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Article

In silico analysis identifies CRISP3 as a potential peripheral blood biomarker for multiple myeloma: From data modeling to validation with RT-PCR

  • Authors:
    • Dong Leng
    • Ran Miao
    • Xiaoxi Huang
    • Ying Wang
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory, Beijing Chao‑Yang Hospital, Capital Medical University, Beijing 100020, P.R. China, Medical Research Center, Beijing Chao‑Yang Hospital, Capital Medical University, Beijing 100020, P.R. China
  • Pages: 5167-5174
    |
    Published online on: February 6, 2018
       https://doi.org/10.3892/ol.2018.7969
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Abstract

Octamer-binding protein 2 (Oct2) binds to the ATGCAAAT octamer on the IgH enhancer and stimulates IgH expression in human multiple myeloma (MM). Cysteine‑rich secreted protein 3 (CRISP3) possesses the ATGCAAAT sequence and thus is activated by Oct2 in mouse B cells, suggesting that CRISP3 may be activated in and be a potential biomarker for MM. The present study involved a meta‑analysis of the gene expression profiling data of human MM peripheral blood. Significantly expressed genes were analyzed on merged super array microarray data and selected sample data with significantly expressed genes were additionally analyzed by principal component analysis and Bayesian probit regression. CRISP3, Oct2, Apha-1B-glycoprotein (A1GB) and Cyclin D2 (CCND2) were validated in clinical MM peripheral blood samples using reverse transcription quantitative polymerase chain reaction. In the gene expression profiling data, CRISP3 was significantly upregulated and had certain proportions on the discriminated principal component of significantly expressed gene sample data. RT-qPCR analysis revealed CRISP3 was significantly upregulated in MM. Therefore, CRISP3 is a potential peripheral blood biomarker for MM.
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1 

Leich E, Weißbach S, Klein HU, Grieb T, Pischimarov J, Stühmer T, Chatterjee M, Steinbrunn T, Langer C, Eilers M, et al: Multiple myeloma is affected by multiple and heterogeneous somatic mutations in adhesion- and receptor tyrosine kinase signaling molecules. Blood Cancer J. 3:e1022013. View Article : Google Scholar : PubMed/NCBI

2 

Schmidt-Hieber M, Gutiérrez ML, Pérez-Andrés M, Paiva B, Rasillo A, Tabernero MD, Sayagués JM, Lopez A, Bárcena P, Sanchez ML, et al: Cytogenetic profiles in multiple myeloma and monoclonal gammopathy of undetermined significance: A study in highly purified aberrant plasma cells. Haematologica. 98:279–287. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Keats JJ, Maxwell CA, Taylor BJ, Hendzel MJ, Chesi M, Bergsagel PL, Larratt LM, Mant MJ, Reiman T, Belch AR and Pilarski LM: Overexpression of transcripts originating from the MMSET locus characterizes all t(4;14)(p16;q32)-positive multiple myeloma patients. Blood. 105:4060–4069. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Toman I, Loree J, Klimowicz AC, Bahlis N, Lai R, Belch A, Pilarski L and Reiman T: Expression and prognostic significance of Oct2 and Bob1 in multiple myeloma: Implications for targeted therapeutics. Leuk Lymphoma. 52:659–667. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Szudyszczyrek A, Szczyrek M, Sorokawojtaszko M and Hus M: New prognostic biomarkers in multiple myeloma. Postepy Hig Med Dosw (Online). 70:811–819. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Pfisterer P, König H, Hess J, Lipowsky G, Haendler B, Schleuning WD and Wirth T: CRISP-3, a protein with homology to plant defense proteins, is expressed in mouse B cells under the control of Oct2. Mol Cell Biol. 16:6160–6168. 1996. View Article : Google Scholar : PubMed/NCBI

7 

Wirth T, Pfisterer P, Annweiler A, Zwilling S and König H: Molecular principals of Oct2-mediated gene activation in B cells. Immunobiology. 193:161–170. 1995. View Article : Google Scholar : PubMed/NCBI

8 

Udby L, Cowland JB, Johnsen AH, Sørensen OE, Borregaard N and Kjeldsen L: An ELISA for SGP28/CRISP-3, a cysteine-rich secretory protein in human neutrophils, plasma, and exocrine secretions. J Immunol Methods. 263:43–55. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Raje N, Woo SB, Hande K, Yap JT, Richardson PG, Vallet S, Treister N, Hideshima T, Sheehy N, Chhetri S, et al: Clinical, radiographic, and biochemical characterization of multiple myeloma patients with osteonecrosis of the jaw. Clin Cancer Res. 14:2387–2395. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Garg TK, Szmania SM, Khan JA, Hoering A, Malbrough PA, Moreno-Bost A, Greenway AD, Lingo JD, Li X, Yaccoby S, et al: Highly activated and expanded natural killer cells for multiple myeloma immunotherapy. Haematologica. 97:1348–1356. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Kiaii S, Clear AJ, Ramsay AG, Davies D, Sangaralingam A, Lee A, Calaminici M, Neuberg DS and Gribben JG: Follicular lymphoma cells induce changes in T-cell gene expression and function: Potential impact on survival and risk of transformation. J Clin Oncol. 31:2654–2661. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Rhodes DR, Barrette TR, Rubin MA, Ghosh D and Chinnaiyan AM: Meta-analysis of microarrays: Interstudy validation of gene expression profiles reveals pathway dysregulation in prostate cancer. Cancer Res. 62:4427–4433. 2002.PubMed/NCBI

13 

Barrett T and Edgar R: Gene expression omnibus: Microarray data storage, submission, retrieval, and analysis. Methods Enzymol. 411:352–369. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Rudy J and Valafar F: Empirical comparison of cross-platform normalization methods for gene expression data. BMC Bioinformatics. 12:4672011. View Article : Google Scholar : PubMed/NCBI

15 

Shabalin AA, Tjelmeland H, Fan C, Perou CM and Nobel AB: Merging two gene-expression studies via cross-platform normalization. Bioinformatics. 24:1154–1160. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Shain KH and Dalton WS: Genetic and environmental determinants in multiple myeloma: Implications for therapy. Springer Netherlands. 14:53–82. 2012.

17 

Mattioli M, Agnelli L, Fabris S, Baldini L, Morabito F, Bicciato S, Verdelli D, Intini D, Nobili L, Cro L, et al: Gene expression profiling of plasma cell dyscrasias reveals molecular patterns associated with distinct IGH translocations in multiple myeloma. Oncogene. 24:2461–2473. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Huang da W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Huang da W, Sherman BT and Lempicki RA: Bioinformatics enrichment tools: Paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res. 37:1–13. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Tusher VG, Tibshirani R and Chu G: Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA. 98:pp. 5116–5121. 2001; View Article : Google Scholar : PubMed/NCBI

22 

Saligan LN, Hsiao CP, Wang D, Wang XM, St John L, Kaushal A, Citrin D, Barb JJ, Munson PJ and Dionne RA: Upregulation of alpha-synuclein during localized radiation therapy signals the association of cancer-related fatigue with the activation of inflammatory and neuroprotective pathways. Brain Behav Immun. 27:63–70. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Canales NA, Marina VM, Castro JS, Jiménez AA, Mendoza-Hernández G, McCARRON EL, Roman MB and Castro-Romero JI: A1BG and C3 are overexpressed in patients with cervical intraepithelial neoplasia III. Oncol Lett. 8:939–947. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Misiewicz-Krzeminska I, Sarasquete ME, Vicente-Dueñas C, Krzeminski P, Wiktorska K, Quwaider D, Rojas EA, Corral R, Corchete LA, Martin AA, et al: Post-Transcriptional modifications explain the overexpression of CCND2 in multiple myeloma. Blood. 124:20012014.PubMed/NCBI

25 

Scheidereit C, Heguy A and Roeder RG: Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro. Cell. 51:783–793. 1987. View Article : Google Scholar : PubMed/NCBI

26 

Staudt LM, Singh H, Sen R, Wirth T, Sharp PA and Baltimore D: A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes. Nature. 323:640–643. 1986. View Article : Google Scholar : PubMed/NCBI

27 

Al Bashir S, Alshalalfa M, Hegazy SA, Dolph M, Donnelly B and Bismar TA: Cysteine-rich secretory protein 3 (CRISP3), ERG and PTEN define a molecular subtype of prostate cancer with implication to patients' prognosis. J Hematol Oncol. 7:212014. View Article : Google Scholar : PubMed/NCBI

28 

Ribeiro FR, Paulo P, Costa VL, Barros-Silva JD, Ramalho-Carvalho J, Jerónimo C, Henrique R, Lind GE, Skotheim RI, Lothe RA and Teixeira MR: Cysteine-rich secretory protein-3 (CRISP3) is strongly Up-regulated in prostate carcinomas with the TMPRSS2-ERG fusion gene. PLoS One. 6:e223172011. View Article : Google Scholar : PubMed/NCBI

29 

Liao Q, Kleeff J, Xiao Y, Guweidhi A, Schambony A, Töpfer-Petersen E, Zimmermann A, Büchler MW and Friess H: Preferential expression of cystein-rich secretory protein-3 (CRISP-3) in chronic pancreatitis. Histol Histopathol. 18:425–433. 2003.PubMed/NCBI

30 

Ko WC, Sugahara K, Sakuma T, Yen CY, Liu SY, Liaw GA and Shibahara T: Copy number changes of CRISP3 in oral squamous cell carcinoma. Oncol Lett. 3:75–81. 2012. View Article : Google Scholar : PubMed/NCBI

31 

López-Corral L, Corchete LA, Sarasquete ME, Mateos MV, García-Sanz R, Fermiñán E, Lahuerta JJ, Bladé J, Oriol A, Teruel AI, et al: Transcriptome analysis reveals molecular profiles associated with evolving steps of monoclonal gammopathies. Haematologica. 99:1365–1372. 2014. View Article : Google Scholar : PubMed/NCBI

32 

André T, Meuleman N, Stamatopoulos B, De Bruyn C, Pieters K, Bron D and Lagneaux L: Evidences of early senescence in multiple myeloma bone marrow mesenchymal stromal cells. PLoS One. 8:e597562013. View Article : Google Scholar : PubMed/NCBI

33 

Grupp K, Kohl S, Sirma H, Simon R, Steurer S, Becker A, Adam M, Izbicki J, Sauter G, Minner S, et al: Cysteine-rich secretory protein 3 overexpression is linked to a subset of PTEN-deleted ERG fusion-positive prostate cancers with early biochemical recurrence. Mod Pathol. 26:733–742. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Leng D, Miao R, Huang X and Wang Y: In silico analysis identifies CRISP3 as a potential peripheral blood biomarker for multiple myeloma: From data modeling to validation with RT-PCR. Oncol Lett 15: 5167-5174, 2018.
APA
Leng, D., Miao, R., Huang, X., & Wang, Y. (2018). In silico analysis identifies CRISP3 as a potential peripheral blood biomarker for multiple myeloma: From data modeling to validation with RT-PCR. Oncology Letters, 15, 5167-5174. https://doi.org/10.3892/ol.2018.7969
MLA
Leng, D., Miao, R., Huang, X., Wang, Y."In silico analysis identifies CRISP3 as a potential peripheral blood biomarker for multiple myeloma: From data modeling to validation with RT-PCR". Oncology Letters 15.4 (2018): 5167-5174.
Chicago
Leng, D., Miao, R., Huang, X., Wang, Y."In silico analysis identifies CRISP3 as a potential peripheral blood biomarker for multiple myeloma: From data modeling to validation with RT-PCR". Oncology Letters 15, no. 4 (2018): 5167-5174. https://doi.org/10.3892/ol.2018.7969
Copy and paste a formatted citation
x
Spandidos Publications style
Leng D, Miao R, Huang X and Wang Y: In silico analysis identifies CRISP3 as a potential peripheral blood biomarker for multiple myeloma: From data modeling to validation with RT-PCR. Oncol Lett 15: 5167-5174, 2018.
APA
Leng, D., Miao, R., Huang, X., & Wang, Y. (2018). In silico analysis identifies CRISP3 as a potential peripheral blood biomarker for multiple myeloma: From data modeling to validation with RT-PCR. Oncology Letters, 15, 5167-5174. https://doi.org/10.3892/ol.2018.7969
MLA
Leng, D., Miao, R., Huang, X., Wang, Y."In silico analysis identifies CRISP3 as a potential peripheral blood biomarker for multiple myeloma: From data modeling to validation with RT-PCR". Oncology Letters 15.4 (2018): 5167-5174.
Chicago
Leng, D., Miao, R., Huang, X., Wang, Y."In silico analysis identifies CRISP3 as a potential peripheral blood biomarker for multiple myeloma: From data modeling to validation with RT-PCR". Oncology Letters 15, no. 4 (2018): 5167-5174. https://doi.org/10.3892/ol.2018.7969
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