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Article

Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1

  • Authors:
    • Baimou Li
    • Xiaopeng Mao
    • Hua Wang
    • Guanyu Su
    • Chengqiang Mo
    • Kaiyuan Cao
    • Shaopeng Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China, Research Center for Clinical Laboratory Standard, Zhongshan Medical School, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China
  • Pages: 5193-5200
    |
    Published online on: February 7, 2018
       https://doi.org/10.3892/ol.2018.7975
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Abstract

The aim of the present study was to investigate the associations between vasculogenic mimicry (VM) and zinc finger E-box binding homeobox 1 (ZEB1) in bladder cancer. VM structure and ZEB1 expression were analyzed by cluster of differentiation 34/periodic acid Schiff (PAS) double staining and immunohistochemical staining in 135 specimens from patients with bladder cancer, and a further 12 specimens from normal bladder tissues. Three-dimensional (3-D) culture was used to detect VM formation in the bladder transitional cancer cell lines UM-UC-3 and J82, and the immortalized human bladder epithelium cell line SV-HUC-1 in vitro. ZEB1 expression in these cell lines was compared by reverse transcription-quantitative polymerase chain reaction and western blot assays. In addition, small interfering RNA was used to inhibit ZEB1 in UM-UC-3 and J82 cells, followed by 3-D culturing of treated cell lines. As a result, VM was observed in 31.1% of specimens from bladder cancer tissues, and cases with high ZEB1 expression accounted for 60.0% of patients with bladder cancer. In addition, ZEB1 expression was closely associated with VM (r=0.189; P<0.05), and also increased as the grade and stage of the tumor developed. In an in vitro assay, UM-UC-3 and J82 cells exhibited VM formation, however, SV-HUC-1 did not. Furthermore, VM-forming cancer cell lines UM-UC-3 and J82 exhibited higher ZEB1 expression. Notably, VM formation was inhibited following knockdown of ZEB1. In conclusion, ZEB1 may be associated with VM in bladder cancer and serve an important role in the process of VM formation. However, its detailed mechanism requires further study.
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Copy and paste a formatted citation
Spandidos Publications style
Li B, Mao X, Wang H, Su G, Mo C, Cao K and Qiu S: Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1. Oncol Lett 15: 5193-5200, 2018.
APA
Li, B., Mao, X., Wang, H., Su, G., Mo, C., Cao, K., & Qiu, S. (2018). Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1. Oncology Letters, 15, 5193-5200. https://doi.org/10.3892/ol.2018.7975
MLA
Li, B., Mao, X., Wang, H., Su, G., Mo, C., Cao, K., Qiu, S."Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1". Oncology Letters 15.4 (2018): 5193-5200.
Chicago
Li, B., Mao, X., Wang, H., Su, G., Mo, C., Cao, K., Qiu, S."Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1". Oncology Letters 15, no. 4 (2018): 5193-5200. https://doi.org/10.3892/ol.2018.7975
Copy and paste a formatted citation
x
Spandidos Publications style
Li B, Mao X, Wang H, Su G, Mo C, Cao K and Qiu S: Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1. Oncol Lett 15: 5193-5200, 2018.
APA
Li, B., Mao, X., Wang, H., Su, G., Mo, C., Cao, K., & Qiu, S. (2018). Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1. Oncology Letters, 15, 5193-5200. https://doi.org/10.3892/ol.2018.7975
MLA
Li, B., Mao, X., Wang, H., Su, G., Mo, C., Cao, K., Qiu, S."Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1". Oncology Letters 15.4 (2018): 5193-5200.
Chicago
Li, B., Mao, X., Wang, H., Su, G., Mo, C., Cao, K., Qiu, S."Vasculogenic mimicry in bladder cancer and its association with the aberrant expression of ZEB1". Oncology Letters 15, no. 4 (2018): 5193-5200. https://doi.org/10.3892/ol.2018.7975
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