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MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3

  • Authors:
    • Yuewen Gao
    • Zhaoyan Liu
    • Zhaohong Ding
    • Shicai Hou
    • Jun Li
    • Kehua Jiang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The People's Hospital of Rizhao City, Rizhao, Shandong 276800, P.R. China, Department of Urology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Enshi, Hubei 445000, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4781-4788
    |
    Published online on: February 7, 2018
       https://doi.org/10.3892/ol.2018.7976
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Abstract

To investigate the effect of microRNA (miR)-155 on colon cancer chemoresistance to cisplatine and its mechanism. Reverse transcription quantitative polymerase chain reaction was used to measure the levels of miR‑155 and forkhead box O3 (FOXO3) in colon cancer specimens and cell lines. Overexpression of miR‑155 and miR‑155 inhibitor were transfected into colon cancer cell lines to investigate its role of chemoresistance to cisplatin in colon cancer. MTS assays were used to analyse cell viability in vitro. In vivo tumor formation assays were performed in C57BL/6 wild type and miR‑155 knockout mice (miR‑155‑/‑). A luciferase reporter assay was used to measure the translation of FOXO3. Additionally, the expression of FOXO3 was detected by western blot analysis. It was identified that miR‑155 was markedly upregulated in colon cancer tissue and cell lines. Overexpression of miR‑155 enhanced colon cancer cell chemoresistance to cisplatin in vitro and tumorigenesis in vivo. In addition, overexpression of miR‑155 was associated with decreased levels of FOXO3, primarily through inhibiting the expression of FOXO3 to increase colon cancer resistanec to cisplatin. The present study demonstrated that miR‑155 increased colon cancer drug resistance and decreased FOXO3 expression in vivo and in vitro. This may provide a novel method for the treatment of drug‑resistant colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Gao Y, Liu Z, Ding Z, Hou S, Li J and Jiang K: MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3. Oncol Lett 15: 4781-4788, 2018.
APA
Gao, Y., Liu, Z., Ding, Z., Hou, S., Li, J., & Jiang, K. (2018). MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3. Oncology Letters, 15, 4781-4788. https://doi.org/10.3892/ol.2018.7976
MLA
Gao, Y., Liu, Z., Ding, Z., Hou, S., Li, J., Jiang, K."MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3". Oncology Letters 15.4 (2018): 4781-4788.
Chicago
Gao, Y., Liu, Z., Ding, Z., Hou, S., Li, J., Jiang, K."MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3". Oncology Letters 15, no. 4 (2018): 4781-4788. https://doi.org/10.3892/ol.2018.7976
Copy and paste a formatted citation
x
Spandidos Publications style
Gao Y, Liu Z, Ding Z, Hou S, Li J and Jiang K: MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3. Oncol Lett 15: 4781-4788, 2018.
APA
Gao, Y., Liu, Z., Ding, Z., Hou, S., Li, J., & Jiang, K. (2018). MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3. Oncology Letters, 15, 4781-4788. https://doi.org/10.3892/ol.2018.7976
MLA
Gao, Y., Liu, Z., Ding, Z., Hou, S., Li, J., Jiang, K."MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3". Oncology Letters 15.4 (2018): 4781-4788.
Chicago
Gao, Y., Liu, Z., Ding, Z., Hou, S., Li, J., Jiang, K."MicroRNA-155 increases colon cancer chemoresistance to cisplatin by targeting forkhead box O3". Oncology Letters 15, no. 4 (2018): 4781-4788. https://doi.org/10.3892/ol.2018.7976
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