Prognostic significance of copy number alterations detected by multi‑link probe amplification of multiple genes in adult acute lymphoblastic leukemia

Fang,Qiuyun; Yuan,Tian; Li,Yan; Feng,Juan; Gong,Xiaoyuan; Li,Qinghua; Zhao,Xingli; Liu,Kaiqi; Tang,Kejing; Tian,Zheng; Zhang,Qi; Wang,Ying; Liu,Bingcheng; Wang,Min; Ru,Kun; Wang,Jianxiang; Mi,Yingchang

doi:10.3892/ol.2018.7985

April-2018 Volume 15 Issue 4

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April-2018 Volume 15 Issue 4

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Article

Prognostic significance of copy number alterations detected by multi‑link probe amplification of multiple genes in adult acute lymphoblastic leukemia

Authors:
- Qiuyun Fang
- Tian Yuan
- Yan Li
- Juan Feng
- Xiaoyuan Gong
- Qinghua Li
- Xingli Zhao
- Kaiqi Liu
- Kejing Tang
- Zheng Tian
- Qi Zhang
- Ying Wang
- Bingcheng Liu
- Min Wang
- Kun Ru
- Jianxiang Wang
- Yingchang Mi
View Affiliations / Copyright

Affiliations: Leukemia Department, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, P.R. China, Hematology Department, Tianjin Cancer Hospital, Tianjin 300060, P.R. China
Pages: 5359-5367
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Published online on: February 7, 2018

https://doi.org/10.3892/ol.2018.7985
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Abstract

The multiplex ligation-dependent probe amplification (MLPA) method was used to detect the copy number alterations (CNAs) of IKAROS family zinc finger 1 (IKZF1), paired box 5 (PAX5), ETS variant 6 (ETV6), RB transcriptional corepressor 1 (RB1), BTG anti‑proliferation factor 1 (BTG1), early B‑cell factor 1 (EBF1), cyclin dependent kinase inhibitor 2A/2B (CDKN2A/2B) and cytokine receptor like factor 2 (CRLF2) genes in 87 adults with acute lymphoblastic leukemia (ALL) in China. The effects of CNAs on prognosis were analyzed. Gene deletions were detected in 58/87 (66.7%) ALL patients. The most common deletions were observed in the following genes: IKZF1 (40.6%), CDKN2A (31.9%), CDKN2B (29%), PAX5 (21.7%), RB1 (14.5%) and BTG1 (10.1%). B cell‑ALL (B‑ALL) patients with CDKN2A/2B deletions exhibited poor 2‑year overall survival (OS; P=0.055) and relapse‑free survival (RFS; P=0.054) rates. CDKN2A/2B deletions were associated with poor 2‑year OS (P=0.045) and RFS (P=0.071) rates in Philadelphia chromosome positive (Ph+) B‑ALL patients, as well as in the high risk (HR) B‑ALL group (P=0.037 and P=0.047, respectively). Patients with PAX5 deletions displayed poor 2‑year OS (P=0.004) and RFS (P=0.016) rates in Philadelphia chromosome negative (Ph‑) B‑ALL patients. Patients with ≥3 gene deletions exhibited a poorer prognosis than other patients (OS, P=0.001; RFS, 0.002).

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Prognostic significance of copy number alterations detected by multi‑link probe amplification of multiple genes in adult acute lymphoblastic leukemia

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