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Article

Significance and prognostic role of human epidermal growth factor receptor 2 and RAB1A expression in gastric cancer

  • Authors:
    • Bihong Xu
    • Chunyu Huang
    • Xianzi Yang
    • Xiangzhao Li
    • Liang Li
    • Yanqing Ding
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Endoscopy, Sun Yat‑sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China
  • Pages: 5185-5192
    |
    Published online on: February 7, 2018
       https://doi.org/10.3892/ol.2018.7992
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Abstract

Human epidermal growth factor receptor 2 (HER-2) has an important clinical role in various cancers. However, the prognostic impact of HER‑2 in gastric cancer (GC) is controversial. RAB1A is an important small molecule in the mechanistic target of rapamycin signalling pathway, which is one of the downstream signalling pathways of the epidermal growth factor receptor family. In recent years, the aberrant expression of RAB1A has been reported in a number of tumours, but its regulation in GC has not been extensively examined. Therefore, the present study investigated the expression pattern and prognostic significance of HER‑2 and RAB1A in gastric adenocarcinoma (CAG). A comprehensive analysis was performed to examine the expression level of HER‑2 and RAB1A in 280 cases of paired paraffin‑embedded GAC tissues and an additional 120 archived GAC tissue samples. HER‑2 and RAB1A protein expression was assessed by immunohistochemistry and cases with a 2+ score for HER‑2 expression levels were subjected to fluorescence in situ hybridization to determine the HER‑2 amplification status. Furthermore, HER‑2 and RAB1A mRNA expression was quantified by reverse transcription‑quantitative polymerase chain reaction. The comparison of continuous data between two groups was performed using a paired‑samples t‑test. Clinical correlations were determined using Pearson's Chi‑square and Fisher's exact tests. Kaplan‑Meier survival curves were used to estimate overall survival (OS). Cox proportional hazards models were used to determine associations between HER‑2 and RAB1A expression and outcomes. Regression analyses were performed to detect the correlation between the mRNA levels of HER‑2 and RAB1A in GAC tissues. It was observed that RAB1A was significantly overexpressed in GAC tissues compared with normal tissues (P<0.001). Approximately 12.86% of the 280 GAC patients had HER‑2 amplification. Additionally, RAB1A expression was significantly associated with a short OS (P<0.001) but there were no significant differences in survival between the HER‑2 high‑expression group and the HER‑2 low‑expression group. Additionally, the co‑expression of HER‑2 and RAB1A indicated poorer OS than the overexpression of each protein (P=0.001), and the two factors were significantly positively correlated in GAC (P=0.012). These findings may be used to further explore the molecular mechanisms and regulatory associations among signalling pathways in GC.
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Copy and paste a formatted citation
Spandidos Publications style
Xu B, Huang C, Yang X, Li X, Li L and Ding Y: Significance and prognostic role of human epidermal growth factor receptor 2 and RAB1A expression in gastric cancer. Oncol Lett 15: 5185-5192, 2018.
APA
Xu, B., Huang, C., Yang, X., Li, X., Li, L., & Ding, Y. (2018). Significance and prognostic role of human epidermal growth factor receptor 2 and RAB1A expression in gastric cancer. Oncology Letters, 15, 5185-5192. https://doi.org/10.3892/ol.2018.7992
MLA
Xu, B., Huang, C., Yang, X., Li, X., Li, L., Ding, Y."Significance and prognostic role of human epidermal growth factor receptor 2 and RAB1A expression in gastric cancer". Oncology Letters 15.4 (2018): 5185-5192.
Chicago
Xu, B., Huang, C., Yang, X., Li, X., Li, L., Ding, Y."Significance and prognostic role of human epidermal growth factor receptor 2 and RAB1A expression in gastric cancer". Oncology Letters 15, no. 4 (2018): 5185-5192. https://doi.org/10.3892/ol.2018.7992
Copy and paste a formatted citation
x
Spandidos Publications style
Xu B, Huang C, Yang X, Li X, Li L and Ding Y: Significance and prognostic role of human epidermal growth factor receptor 2 and RAB1A expression in gastric cancer. Oncol Lett 15: 5185-5192, 2018.
APA
Xu, B., Huang, C., Yang, X., Li, X., Li, L., & Ding, Y. (2018). Significance and prognostic role of human epidermal growth factor receptor 2 and RAB1A expression in gastric cancer. Oncology Letters, 15, 5185-5192. https://doi.org/10.3892/ol.2018.7992
MLA
Xu, B., Huang, C., Yang, X., Li, X., Li, L., Ding, Y."Significance and prognostic role of human epidermal growth factor receptor 2 and RAB1A expression in gastric cancer". Oncology Letters 15.4 (2018): 5185-5192.
Chicago
Xu, B., Huang, C., Yang, X., Li, X., Li, L., Ding, Y."Significance and prognostic role of human epidermal growth factor receptor 2 and RAB1A expression in gastric cancer". Oncology Letters 15, no. 4 (2018): 5185-5192. https://doi.org/10.3892/ol.2018.7992
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